A5079888845- Parkinson's Disease Mechanisms and Treatments
- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Neuroscience and Neural Engineering
- Neurological disorders and treatments
- Carbohydrate Chemistry and Synthesis
- Pluripotent Stem Cells Research
- Ovarian cancer diagnosis and treatment
- Nerve injury and regeneration
- Photoreceptor and optogenetics research
- PARP inhibition in cancer therapy
- Autism Spectrum Disorder Research
- Cancer Cells and Metastasis
- Ubiquitin and proteasome pathways
- Genetic Neurodegenerative Diseases
- FOXO transcription factor regulation
- TGF-β signaling in diseases
- Drug Transport and Resistance Mechanisms
- Cellular Mechanics and Interactions
- Alzheimer's disease research and treatments
- Caveolin-1 and cellular processes
- Aluminum toxicity and tolerance in plants and animals
- Biotin and Related Studies
- Toxin Mechanisms and Immunotoxins
- Calcium signaling and nucleotide metabolism
Université Laval
2014-2024
Centre hospitalier universitaire de Québec
2019-2023
Wilfrid Laurier University
2022
Hôtel-Dieu de Québec
2016-2017
University of Lausanne
2014
University of British Columbia
2009
University of Missouri–Kansas City
2002
Abstract Accumulation of α-synuclein aggregates in the substantia nigra pars compacta is central pathophysiology Parkinson’s disease, leading to degeneration dopaminergic neurons and manifestation motor symptoms. Although several PD models mimic pathological accumulation after overexpression, they do not allow for controlling monitoring its aggregation. We recently generated a new optogenetic tool by which we can spatiotemporally control aggregation using light-induced protein system. Using...
Neurodegenerative disorders refer to a group of diseases commonly associated with abnormal protein accumulation and aggregation in the central nervous system. However, exact role pathophysiology these remains unclear. This gap knowledge is due lack experimental models that allow for spatiotemporal control aggregation, investigation early dynamic events inclusion formation. Here, we report on development light-inducible (LIPA) system enables α-synuclein (α-syn) into insoluble deposits called...
Previously, we have identified the Grainyhead transcription factor 2 gene (GRHL2) as notably hypomethylated in high-grade (HG) serous epithelial ovarian tumors, compared with normal tissues. GRHL2 is known for its functions tissue development and wound healing. In context of cancer, role still ambiguous both tumorigenic tumor suppressive been reported this gene, although a maintaining status cancer cells has suggested. study, report that strongly overexpressed low malignant potential (LMP)...
Protein glycosylation perturbations are implicated in a variety of diseases, including cancer. Aberrant cancer is frequently attributed to altered expression polypeptide GalNAc transferases (GalNAc‑Ts) - enzymes initiating mucin-type O-glycosylation. A previous study from our group demonstrated that one member this family (GALNT3) overexpressed epithelial ovarian (EOC), and GALNT3 correlated with shorter progression-free survival (PFS) EOC patients advanced disease. As considerable degree...
Abstract The use of human derived induced pluripotent stem cells (hiPSCs) differentiated to dopaminergic (DA) neurons offers a valuable experimental model decorticate the cellular and molecular mechanisms Parkinson’s disease (PD) pathogenesis. However, existing approaches present with several limitations, notably lengthy time course protocols high variability in yield DA neurons. Here we report on development an improved approach that combines neurogenin-2 programming commercially available...
Neuronal morphology plays an essential role in neuronal function. The establishment and maintenance of is intimately linked to the actin cytoskeleton; however, molecular mechanisms that regulate changes are poorly understood. Here we identify a novel myosin-Va (MyoVa)-interacting protein, RILPL2, which regulates cellular morphology. Overexpression this protein young or mature hippocampal neurons results increase number spine-like protrusions. By contrast, knockdown endogenous RILPL2 by short...
Poly(ADP-ribose) polymerase inhibitors (PARPis) specifically target homologous recombination deficiency (HRD) cells and display good therapeutic effect in women with advanced-stage BRCA1/2-mutated breast epithelial ovarian cancer (EOC). However, about 50% of high grade serous cancers (HGSOC) present HRD due to epigenetic BRCA1 inactivation, as well genetic/epigenetic inactivation(s) other HR genes, a feature known "BRCAness". Therefore, there is potential for extending the use PARPis these...
Generation of functional human dopaminergic (DA) neurons from induced pluripotent stem cells (hiPSCs) is a crucial tool for modeling dopamine-related diseases and cell replacement therapies. Here, we present protocol to combine neuralizing transcription factor (NGN2) programming DA patterning differentiate hiPSCs into mature (iDA) neurons. We describe steps transduction neural induction through differentiation maturation near-pure, fully iDA within 3 weeks. For complete details on the use...
Epithelial ovarian cancer (EOC) represents the most lethal gynecologic malignancy; a better understanding of molecular mechanisms associated with EOC etiology could substantially improve management. Aberrant O-glycosylation in is attributed to alteration N-acetylgalactosaminyltransferases (GalNAc-Ts). Reports suggest genetic and functional redundancy between GalNAc-Ts, our previous data are indicative an induction GALNT6 expression upon GALNT3 suppression cells. We performed single double...
The molecular basis of epithelial ovarian cancer (EOC) dissemination is still poorly understood. We have previously identified the hydrogen peroxide-inducible clone-5 (Hic-5) gene as hypomethylated in high-grade (HG) serous EOC tumors, compared to normal tissues. Hic-5 a focal adhesion scaffold protein and has been primarily studied for its role key mediator TGF-β-induced epithelial-to-mesenchymal transition (EMT) cells both malignant origin; however, never investigated. Here we demonstrate...
This article contains raw and processed data related to research published in "Role of the polypeptide N-acetylgalactosaminyltransferase 3 ovarian cancer progression: possible implications abnormal mucin O-glycosylation" [1]. The presented here was obtained with application a bioorthogonal chemical reporter strategy analyzing differential glycoprotein expression following knock-down (KD) GALNT3 gene epithelial (EOC) cell line A2780s. LC-MS/MS mass spectrometry analysis then performed...
Epithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy, and understanding molecular changes associated with EOC etiology could lead to identification of novel targets for more effective therapeutic interventions. Glycosylation represents a post-translational modification (PTM) proteins playing major role in various cellular functions. Moreover, glycosylation participates pathobiological events during tumor progression, as aberrant expression glycan structures has been...
Alpha-synuclein (α-syn) aggregation is a defining feature of Parkinson's disease (PD) and related synucleinopathies. Despite significant research efforts focused on understanding α-syn mechanisms, the early stages this process remain elusive, largely due to limitations in experimental tools that lack temporal resolution capture these dynamic events. Here, we introduce UltraID-LIPA, an innovative platform combines Light-Inducible Protein Aggregation (LIPA) system with UltraID...
Parkinson's disease (PD) is a prevalent neurodegenerative disorder characterized by the degeneration of dopamine neurons in substantia nigra pars compacta, leading to motor and non-motor symptoms. While symptoms such as rigidity, tremor, bradykinesia/akinesia, postural instability are well-recognized, including cognitive decline, depression, anxiety also significantly impact patients' quality life. Preclinical research utilizing animal models has been instrumental understanding PD...
α-synuclein (α-syn) aggregation into insoluble deposits, referred to as Lewy bodies (LBs) is the paramount pathological hallmark of Parkinson's disease and related α-synucleinopathies. However, how these aggregates affect neuronal homeostasis leading neurodegeneration remains elusive. This gap in knowledge mainly due lack proper cellular animal models undertake such investigations. We have addressed this by developing a light-inducible protein (LIPA) system that allows for real-time...
α-Synuclein (α-syn) aggregation into insoluble deposits, referred to as Lewy bodies (LBs), is the paramount pathological hallmark of Parkinson's disease (PD) and related α-synucleinopathies. However, mechanism by which these aggregates contribute neurodegeneration remains unclear. This gap in knowledge due lack experimental models that allow for spatiotemporal control α-syn investigation early dynamic events associated with inclusion formation. Here, we report on development a...
Studying Parkinson's disease (PD) is complex due to a lack of cellular models mimicking key aspects protein pathology. Here, we present protocol for inducing and monitoring α-synuclein aggregation in living cells using optogenetics. We describe steps plasmid transduction, biochemical validation, immunocytochemistry, live-cell confocal imaging. These induced aggregates fulfill the cardinal features authentic inclusions observed PD-diseased brains offer tool study role neurodegeneration. For...