A5101995566- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
- Nanofabrication and Lithography Techniques
- Force Microscopy Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Monoclonal and Polyclonal Antibodies Research
- Cell Image Analysis Techniques
- Microtubule and mitosis dynamics
- Microfluidic and Capillary Electrophoresis Applications
- Mechanical and Optical Resonators
- Micro and Nano Robotics
- Polymer Surface Interaction Studies
- Pluripotent Stem Cells Research
- Blood properties and coagulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Radiation Therapy and Dosimetry
- Ion-surface interactions and analysis
- Immunotherapy and Immune Responses
- Advanced Biosensing Techniques and Applications
- Diffusion and Search Dynamics
- Photoreceptor and optogenetics research
- Cancer Research and Treatments
Eötvös Loránd University
2014-2025
Nanobiosensorics Laboratory
2014-2022
HUN-REN Centre for Energy Research
2016-2022
Montavid Thermodynamic Research Group
2014-2022
Institute for Technical Physics and Materials Science
1984-2019
Hungarian Academy of Sciences
2014-2019
HUN-REN Research Centre for Natural Sciences
2014-2018
We have recorded the swarming-like collective migration of a large number keratocytes (tissue cells obtained from scales goldfish) using long-term videomicroscopy. By increasing overall density migrating cells, we been able to demonstrate experimentally kinetic phase transition disordered into an ordered state. Near critical complex picture emerges with interacting clusters moving in groups. Motivated by these experiments constructed flocking model that exhibits continuous phase, while...
Neurotropic herpesviruses can establish lifelong infection in humans and contribute to severe diseases including encephalitis neurodegeneration. However, the mechanisms through which brain's immune system recognizes controls viral infections propagating across synaptically linked neuronal circuits have remained unclear. Using a well-established model of alphaherpesvirus that reaches brain exclusively via retrograde transsynaptic spread from periphery, vivo two-photon imaging combined with...
A novel high-throughput label-free resonant waveguide grating (RWG) imager biosensor, the Epic® BenchTop (BT), was utilized to determine dependence of cell spreading kinetics on average surface density (vRGD) integrin ligand RGD-motifs. vRGD tuned over four orders magnitude by co-adsorbing biologically inactive PLL-g-PEG and RGD-functionalized PLL-g-PEG-RGD synthetic copolymers from their mixed solutions onto sensor surface. Using highly adherent human cervical tumor (HeLa) cells as a model...
Abstract Single-cell adhesion force plays a crucial role in biological sciences, however its in-depth investigation is hindered by the extremely low throughput and lack of temporal resolution present techniques. While atomic microcopy (AFM) based methods are capable directly measuring detachment values between individual cells substrate, their limited to few per day, cannot provide kinetic evaluation over timescale several hours. In this study high spatial resonant waveguide grating...
TPPP/p25 (tubulin polymerization-promoting protein/p25) is an unstructured protein that induces microtubule polymerization in vitro and aligned along the network transfected mammalian cells. In normal human brain, expressed predominantly oligodendrocytes, where its expression proved to be crucial for their differentiation process. Here we demonstrated of HeLa cells, doxycycline-inducible CHO10 oligodendrocyte CG-4 cells promoted acetylation alpha-tubulin at residue Lys-40, whereas...
Complement receptors CR3 (CD11b/CD18) and CR4 (CD11c/CD18) belong to the family of beta2 integrins are expressed mainly by myeloid cell types in humans. Previously, we proved that rather than plays a key role phagocytosis. Here analysed how CD11b CD11c participate adhesion fibrinogen, common ligand CR4, employing human monocytes, monocyte-derived macrophages (MDMs) dendritic cells (MDDCs) highly expressing as well CD11c. We determined exact numbers on these bead-based technique, found ratio...
Genetically encoded voltage indicators (GEVIs) allow for the cell-type-specific real-time imaging of neuronal membrane potential dynamics, which is essential to understanding information processing at both cellular and circuit levels. Among GEVIs, near-infrared-shifted GEVIs offer faster kinetics, better tissue penetration, compatibility with optogenetic tools, enabling all-optical electrophysiology in complex biological contexts. In our previous work, we employed directed molecular...
Astroglial cells are essential components of the neurogenic niches within central nervous system. Emerging evidence suggests that they among key regulators postnatal neurogenesis. Although astrocytes have been demonstrated to possess potential instruct stem adopt a neuronal fate, little is known about nature glia-derived instructive signals. Here we propose all-trans retinoic acid, one most powerful morphogenic molecules regulating cell fate commitment, may be factors directing...
We report the assessment of sensitivity a microplate-compatible resonant waveguide grating imager. The to bulk refractive index changes was determined using serial dilution glycerol solution with help refractometer. surface examined layer-by-layer polyelectrolyte films in conjunction optical lightmode spectroscopy and characterized by binding acetazolamide immobilized carbonic anhydrase under microfluidics. results suggest that imager has limit detection down 2.2 × 10−6 for change 0.078...
Fluorescence-activated cell sorting (FACS) applying flow cytometry to separate cells on a molecular basis is widespread method. We demonstrate that both fluorescent and unlabeled live in Petri dish observed with microscope can be automatically recognized by computer vision picked up computer-controlled micropipette. This method routinely applied as FACS down the single level very high selectivity. Sorting resolution, i.e., minimum distance between two from which one could selectively removed...
Abstract Current robots can manipulate only surface-attached cells seriously limiting the fields of their application for single cell handling. We developed a computer vision-based robot applying motorized microscope and micropipette to recognize gently isolate intact individual subsequent analysis, e.g., DNA/RNA sequencing in 1–2 nanoliters from thin (~100 μm) layer suspension. It retrieve rare cells, needs minimal sample preparation be applied virtually any tissue type. Combination 1 μm...
CR3 and CR4, the leukocyte specific β2-integrins, involved in cellular adherence, migration phagocytosis, are often assumed to have similar functions. Previously however, we proved that under physiological conditions CR4 is dominant adhesion fibrinogen of human monocyte-derived macrophages (MDMs) dendritic cells (MDDCs). Here, using inflammatory conditions, provide further evidence expression function not identical these cell types. We found LPS treatment changes their differently on MDMs...
Cell adhesion is a fundamental phenomenon vital for all multicellular organisms. Recognition of and to specific macromolecules crucial task leukocytes initiate the immune response. To gain statistically reliable information cell adhesion, large numbers cells should be measured. However, direct measurement force single still challenging today’s techniques typically have an extremely low throughput (5–10 per day). Here, we introduce computer controlled micropipette mounted onto normal inverted...
There is no effective treatment for the loss of functional salivary tissue after irradiation head and neck cancer or autoimmune disease Sjögren's syndrome. One possible approach regeneration glands from stem cells. The present study aimed to investigate whether small pieces human submandiblar gland contain elements necessary reconstruction rudiments in vitro via acinar ductal cell differentiation. Primary submandibular (primary total gland; PTHSG) cells were isolated cultured using a new...
A high-throughput label-free resonant waveguide grating biosensor, the Epic BenchTop, was utilized to in situ monitor adhesion process of cancer cells on Arg-Gly-Asp tripeptide displaying biomimetic polymer surfaces. Using highly adherent human cervical adenocarcinoma (HeLa) as a model system, cell kinetic data with outstanding temporal resolution were obtained. We found that pre-exposing various concentrations main extract green tea, (−)-epigallocatechin gallate (EGCG), largely affected...
Abstract The high throughput, cost effective and sensitive quantification of cell adhesion strength at the single-cell level is still a challenging task. force between tissue cells their environment crucial in all multicellular organisms. Integrins transmit intracellular cytoskeleton extracellular matrix. This not only mechanical interaction but way signal transduction as well. For instance, adhesion-dependent switch to an apoptotic mode lack forces. Adhesion tumor potential therapeutic...
Automated manipulation and sorting of single cells are challenging, when intact needed for further investigations, e.g., RNA or DNA sequencing. We applied a computer controlled micropipette on microscope admitting 80 PCR (Polymerase Chain Reaction) tubes to be filled with in cycle. Due the Laplace pressure, fluid starts flow out from only above critical pressure preventing precise control drop volume submicroliter range. found an anomalous additive that we attribute evaporation drop. have...
Monocytes, dendritic cells (DCs), and macrophages (MFs) are closely related immune that differ in their main functions. These specific functions are, to a considerable degree, determined by the differences adhesion behavior of cells. To study inherently essentially dynamic aspects monocytes, DCs, MFs, cell assays were performed with high-throughput label-free optical biosensor [Epic BenchTop (BT)] on surfaces coated either fibrinogen (Fgn) or biomimetic copolymer PLL-g-PEG-RGD. Cell profiles...
Because of its relevance to everyday life, the spreading viral infections has been central interest in a variety scientific communities involved fighting, preventing and theoretically interpreting epidemic processes. Recent large scale observations have resulted major discoveries concerning overall features process systems with highly mobile susceptible units, but virtually no data are available about infection for very number immobile units. Here we present first detailed quantitative...