A5013083618- Adenosine and Purinergic Signaling
- Neuroscience and Neuropharmacology Research
- Animal Genetics and Reproduction
- Tryptophan and brain disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Protein Hydrolysis and Bioactive Peptides
- Parkinson's Disease Mechanisms and Treatments
- Virus-based gene therapy research
- Nicotinic Acetylcholine Receptors Study
- Neurotransmitter Receptor Influence on Behavior
- Neurological disorders and treatments
- Proteins in Food Systems
- Photoreceptor and optogenetics research
- Monoclonal and Polyclonal Antibodies Research
- Pain Mechanisms and Treatments
- CRISPR and Genetic Engineering
- Adolescent and Pediatric Healthcare
- Neuroscience of respiration and sleep
- Adipose Tissue and Metabolism
- Neuroendocrine regulation and behavior
- Bipolar Disorder and Treatment
- Neuropeptides and Animal Physiology
- Infant Nutrition and Health
HUN-REN Institute of Experimental Medicine
2015-2025
Hungarian Research Network
2024
Hungarian Academy of Sciences
2009-2019
Medical University of Graz
2015-2017
Agricultural Biotechnology Institute
1996-2015
National Agricultural Research and Innovation Centre
2015
ImmunoGenes (Hungary)
2011-2013
University of Vienna
1999
Institute of Genetics
1991
HUN-REN Szegedi Biológiai Kutatóközpont
1991
Microglia are the main immune cells in brain and have roles homeostasis neurological diseases. Mechanisms underlying microglia-neuron communication remain elusive. Here, we identified an interaction site between neuronal cell bodies microglial processes mouse human brain. Somatic junctions a specialized nanoarchitecture optimized for purinergic signaling. Activity of mitochondria was linked with junction formation, which induced rapidly response to activation blocked by inhibition P2Y12...
Microglia, the main immunocompetent cells of brain, regulate neuronal function, but their contribution to cerebral blood flow (CBF) regulation has remained elusive. Here, we identify microglia as important modulators CBF both under physiological conditions and during hypoperfusion. Microglia establish direct, dynamic purinergic contacts with in neurovascular unit that shape mice humans. Surprisingly, absence or blockade microglial P2Y12 receptor (P2Y12R) substantially impairs coupling mice,...
Neurotropic herpesviruses can establish lifelong infection in humans and contribute to severe diseases including encephalitis neurodegeneration. However, the mechanisms through which brain's immune system recognizes controls viral infections propagating across synaptically linked neuronal circuits have remained unclear. Using a well-established model of alphaherpesvirus that reaches brain exclusively via retrograde transsynaptic spread from periphery, vivo two-photon imaging combined with...
Recent investigations have revealed that the genetic deletion of P2X7 receptors (P2rx7) results in an antidepressant phenotype mice. However, link between deficiency P2rx7 and changes behavior has not yet been explored. In present study, we studied effect on neurochemical hippocampus might underlie phenotype. receptor deficient mice (P2rx7−/−) displayed decreased immobility tail suspension test (TST) attenuated anhedonia response sucrose preference (SPT) following bacterial endotoxin (LPS)...
SpringerPlus 2015, 4(Suppl 1):L1 MicroRNAs (miRNAs) are short, 22-25 nucleotide long transcripts that may suppress entire signaling pathways by interacting with the 3'-untranslated region (3'-UTR) of coding mRNA targets, interrupting translation and inducing degradation these targets.The 3'-UTRs brain compared to other tissues predict important roles for miRNAs.Supporting this notion, we found miRNAs co-evolved their target transcripts, non-coding pseudogenes miRNA recognition elements...
The purpose of this study was to explore how genetic deletion and pharmacological antagonism the P2X7 receptor (P2rx7) alter mood-related behaviour, gene expression stress reactivity in brain. forced swim test (FST), tail suspension (TST) amphetamine-induced hyperlocomotion (AH) tests were used wild-type (P2rx7(+/+)) P2rx7-deficient (P2rx7(-/-)) mice. Biogenic amine levels analysed amygdala striatum, adrenocorticotropic hormone (ACTH) corticosterone measured plasma pituitary after restraint...
In this study the role of P2Y12 receptors (P2Y12R) was explored in rodent models inflammatory and neuropathic pain acute thermal nociception. correlation with their activity to block recombinant human P2Y12R, majority P2Y12R antagonists alleviated mechanical hyperalgesia dose-dependently, following intraplantar CFA injection, after partial ligation sciatic nerve rats. They also caused an increase nociceptive threshold hot plate test. Among six evaluated studies, selective receptor antagonist...
Mitochondrial dysfunction, oxidative stress and their interplay are core pathological features of Parkinson's disease. In dopaminergic neurons, monoamines metabolites provide an additional source reactive free radicals during breakdown by monoamine oxidase or auto-oxidation. Moreover, mitochondrial dysfunction have a supraadditive impact on the pathological, cytoplasmic accumulation dopamine its subsequent release. Here we report effects novel series potent selective MAO-B inhibitory...
GPR3 is an orphan G protein-coupled receptor endowed with constitutive Gs signaling activity, which expressed broadly in the central nervous system, maximal expression habenula. We investigated consequences of its genetic deletion several behavioral paradigms and on neurotransmission. Compared to wild-type, hippocampal neurons from Gpr3−/− mice displayed lower basal intracellular cAMP levels, consistent strong activity transiently transfected cells. Behavioral analyses revealed that...
Background Major depression is a well-known risk factor for cardiovascular diseases and increased mortality following myocardial infarction. However, biomarkers of are still missing. The aim this prospective study was to evaluate, whether nitric-oxide (NO) related factors endothelial dysfunction, such as global arginine bioavailability, arginase activity, L-arginine/ADMA ratio the metabolites asymmetric dimethylarginine (ADMA) symmetric (SDMA) might be depression-induced risk. Methods In 71...
Maternal immune activation (MIA) is a principal environmental risk factor contributing to autism spectrum disorder (ASD), which compromises fetal brain development at critical periods of pregnancy and might be causally linked ASD symptoms. We report that endogenous the purinergic ion channel P2X7 (P2rx7) necessary sufficient transduce MIA autistic phenotype in male offspring. induced by poly(I:C) injections P2rx7 WT mouse dams elicited an autism-like their offspring, these alterations were...
At high levels, extracellular ATP operates as a "danger" molecule under pathologic conditions through purinergic receptors, including the ionotropic P2X7 receptor (P2X7R). Its endogenous activation is associated with neurodevelopmental disorders; however, its function during early embryonic stages remains largely unclear. Our objective was to determine role of P2X7R in regulation neuronal outgrowth. For this purpose, we performed Sholl analysis dendritic branches on primary hippocampal...
Matrilin 1, or cartilage matrix protein, is a member of novel family extracellular proteins. To date, four members the have been identified, but their biological role unknown. 1 and matrilin 3 are expressed in cartilage, while 2 4 present many tissues. Here we describe generation analysis mice carrying null mutation Crtm gene encoding 1. Anatomical histological studies demonstrated normal development homozygous mutant mice. Northern blot biochemical analyses show no compensatory...
Abstract Background Previous studies indicate a role of P2X 7 receptors in processes that lead to neuronal death. The main objective our study was examine whether genetic deletion or pharmacological blockade influenced dopaminergic cell death various models Parkinson's disease (PD). Results mRNA encoding and 4 up-regulated after treatment PC12 cells with 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP). antagonists protected against MPTP rotenone induced toxicity the LDH assay, but...
Abstract Parkinson’s disease (PD) is the second most common neurodegenerative worldwide. Recently long non-coding RNAs (lncRNAs) have emerged as possible molecular hubs in diverse pathomechanisms of disease. Among them, NEAT1 gained particular interest due to findings suggesting both protective and deleterious effects this lncRNA PD models.The aim study was clarify some contradictions among data that appeared recent publications concerning effects. For this, we determined whether...