A5077289097- Neuroendocrine regulation and behavior
- Stress Responses and Cortisol
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Memory and Neural Mechanisms
- Circadian rhythm and melatonin
- Neuroscience and Neural Engineering
- Parkinson's Disease Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurobiology and Insect Physiology Research
- Insect behavior and control techniques
- Insect Resistance and Genetics
- Nerve injury and regeneration
- Tryptophan and brain disorders
- Neurological Disease Mechanisms and Treatments
- Neurological disorders and treatments
- Cellular transport and secretion
- CRISPR and Genetic Engineering
- Autism Spectrum Disorder Research
- Tactile and Sensory Interactions
- Immune cells in cancer
- Maternal Mental Health During Pregnancy and Postpartum
- Adenosine and Purinergic Signaling
- Barrier Structure and Function Studies
The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology
2023-2025
Neuroscience Institute
2024
Czech Academy of Sciences, Institute of Experimental Medicine
2017-2024
HUN-REN Institute of Experimental Medicine
2014-2023
Semmelweis University
2014-2022
Hungarian Academy of Sciences
2014-2022
Microglia, the main immunocompetent cells of brain, regulate neuronal function, but their contribution to cerebral blood flow (CBF) regulation has remained elusive. Here, we identify microglia as important modulators CBF both under physiological conditions and during hypoperfusion. Microglia establish direct, dynamic purinergic contacts with in neurovascular unit that shape mice humans. Surprisingly, absence or blockade microglial P2Y12 receptor (P2Y12R) substantially impairs coupling mice,...
Perception, a cognitive construct, emerges through sensorimotor integration (SMI). The genetic mechanisms that shape SMI required for perception are unknown. Here, we demonstrate in mice expression of the autism/intellectual disability gene, Syngap1, cortical excitatory neurons is formation somatomotor networks promote SMI-mediated perception. Cortical Syngap1 was necessary and sufficient setting tactile sensitivity, sustaining object exploration, promoting learning. Mice with deficient...
An important question in behavioral neurobiology is how particular neuron populations and pathways mediate the overall roles of brain structures. Here we investigated this issue by studying medial prefrontal cortex (mPFC), an established locus inhibitory control aggression. We male rats that dominantly distinct mPFC project to produce dense fiber networks with glutamate release sites mediobasal hypothalamus (MBH) lateral (LH; i.e., two executory centers species-specific violent bites,...
Studying animal models furthers our understanding of Parkinson's disease (PD) pathophysiology by providing tools to investigate detailed molecular, cellular and circuit functions. Different versions the neurotoxin-based 6-hydroxydopamine (6-OHDA) model PD have been widely used in rats. However, these typically assess result extensive definitive dopaminergic lesions that reflect a late stage PD, leading paucity studies consequential gap knowledge regarding initial stages, which early...
Glutamatergic neurons, characterized by vesicular glutamate transporters (VGluT1-3) provide the main excitation in brain. Their disturbances have been linked to various brain disorders, which could be also modeled contextual fear test rodents. We aimed characterize participation of VGluT3 development through its contribution hypothalamic-pituitary-adrenocortical axis (HPA) regulation using knockout (KO) mice. Contextual conditioning was induced foot shock and mice were examined 1 7 d later...
Serotonergic mechanisms hosted by raphe nuclei have important roles in affiliative and agonistic behaviors but the separate of two are poorly understood. Here we studied dorsal (DR) median region (MRR) aggression optogenetically stimulating nuclei. Mice received three 3 min-long stimulations, which were separated non-stimulation periods min. The stimulation MRR decreased a phasic-like manner. Effects rapidly expressed during vanished similarly fast when stimulations halted. No carryover...
Serotonergic and glutamatergic neurons of median raphe region (MRR) play a pivotal role in the modulation affective cognitive functions. These synapse both onto themselves remote cortical areas. P2X7 receptors (P2rx7) are ligand gated ion channels expressed by central presynaptic excitatory nerve terminals involved regulation neurotransmitter release. P2rx7s implicated various neuropsychiatric conditions such as schizophrenia depression. Here we investigated whether 5-HT release released...
Electrophysiology provides a direct readout of neuronal activity at temporal precision only limited by the sampling rate. However, interrogating deep brain structures, implanting multiple targets or aiming unusual angles still poses significant challenges for operators, and errors are discovered post-hoc histological reconstruction. Here, we propose method combining high-resolution information about bone landmarks provided micro-CT scanning with soft tissue contrast MRI, which allowed us to...
Abstract Experiments aiming to understand sensory-motor systems, cognition and behavior necessitate training animals perform complex tasks. Traditional protocols require lab personnel move the between home cages chambers, start end sessions, in some cases, hand-control each trial. Human labor not only limits amount of per day, but also introduces several sources variability may increase animal stress. Here we present an automated system for 5-choice serial reaction time task (5CSRTT), a...
The median raphe region (MRR) is believed to control the fear circuitry indirectly, by influencing encoding and retrieval of memories amygdala, hippocampus prefrontal cortex. Here we show that in addition this established role, MRR stimulation may alone elicit emergence remote but not recent memories. We substituted electric shocks with optic C57BL/6N male mice an optogenetic conditioning paradigm found stimulations produced agitation, fear, during trial. Contextual reflected freezing was...
Stress and related disorders are in the focus of interest glutamate is one most important neurotransmitters that can affect these processes. Glutamatergic neurons characterized by vesicular transporters (VGluT1-3) among which vGluT3 unique contributing to non-canonical, neuromodulatory effect glutamate. We aimed study role stress axis regulation anxiety during early postnatal period using knockout (KO) mice with special on sex differences. Anxiety was explored day (PND) 7-8 maternal...
Abstract Microglia, the main immunocompetent cells of brain regulate neuronal function in health and disease, but their contribution to cerebral blood flow (CBF) remained elusive. Here we identify microglia as important modulators CBF both under physiological conditions during hypoperfusion. We show that establish direct purinergic contacts with neurovascular unit shape perfusion mice humans. Surprisingly, absence or blockade microglial P2Y12 receptor (P2Y12R) substantially impairs coupling...
The extinction of conditioned fear is frequently used in laboratories as a model for human exposure therapy and crucial studies posttraumatic stress disorder (PTSD). However, the efficacy specific protocols can vary greatly, underlying brain mechanisms are not sufficiently clarified. To address this issue, variable starting time (one or twenty-eight days after conditioning) were used, durability also studied. Changes behavior, hormone levels neuronal activation patterns stressed rats...
The relevance of vasopressin (AVP) magnocellular origin to the regulation endocrine stress axis and related behaviour is still under discussion. We aimed obtain deeper insight into this process. To rescue AVP synthesis, a vasopressin-containing adeno-associated virus vector (AVP-AAV) was injected supraoptic nucleus (SON) AVP-deficient Brattleboro rats (di/di). compared +/+, di/di, AVP-AAV treated di/di male rats. treatment rescued synthesis in SON both morphologically functionally. It also...
Abstract Experiments aiming to understand sensory-motor systems, cognition and behavior often require animals trained perform complex tasks. Traditional training protocols lab personnel move the between home cages chambers, start end sessions, in some cases, hand-control each trial. Human labor not only limits amount of per day, but also introduces several sources variability may increase animal stress. Here we present an automated system for 5-choice serial reaction time task (5CSRTT), a...