A5019409092- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cannabis and Cannabinoid Research
- Photoreceptor and optogenetics research
- Immune cells in cancer
- Adenosine and Purinergic Signaling
- Immune Response and Inflammation
- Long-Term Effects of COVID-19
- Receptor Mechanisms and Signaling
- Anesthesia and Neurotoxicity Research
- Memory and Neural Mechanisms
- Barrier Structure and Function Studies
- Cell Image Analysis Techniques
- Olfactory and Sensory Function Studies
- Neurological Disease Mechanisms and Treatments
- Genetics and Neurodevelopmental Disorders
- Sleep and Wakefulness Research
- Neurotransmitter Receptor Influence on Behavior
- Nicotinic Acetylcholine Receptors Study
- COVID-19 Clinical Research Studies
- Inflammasome and immune disorders
- Amino Acid Enzymes and Metabolism
HUN-REN Institute of Experimental Medicine
2011-2025
Hungarian Academy of Sciences
2005-2016
Microglia are the main immune cells in brain and have roles homeostasis neurological diseases. Mechanisms underlying microglia-neuron communication remain elusive. Here, we identified an interaction site between neuronal cell bodies microglial processes mouse human brain. Somatic junctions a specialized nanoarchitecture optimized for purinergic signaling. Activity of mitochondria was linked with junction formation, which induced rapidly response to activation blocked by inhibition P2Y12...
Microglia, the main immunocompetent cells of brain, regulate neuronal function, but their contribution to cerebral blood flow (CBF) regulation has remained elusive. Here, we identify microglia as important modulators CBF both under physiological conditions and during hypoperfusion. Microglia establish direct, dynamic purinergic contacts with in neurovascular unit that shape mice humans. Surprisingly, absence or blockade microglial P2Y12 receptor (P2Y12R) substantially impairs coupling mice,...
COVID-19 is associated with diverse neurological abnormalities, but the underlying mechanisms are unclear. We hypothesized that microglia, resident immune cells of brain, centrally involved in this process. To study this, we developed an autopsy platform allowing integration molecular anatomy, protein and mRNA datasets postmortem mirror blocks brain peripheral organ samples from cases COVID-19. observed focal loss microglial P2Y12R, CX3CR1-CX3CL1 axis deficits metabolic failure at sites...
GABAergic inhibition plays a central role in the control of pyramidal cell ensemble activities; thus, any signaling mechanism that regulates is able to fine-tune network patterns. Here, we provide evidence retrograde nitric oxide (NO)–cGMP cascade triggered by NMDA receptor (NMDAR) activation hippocampal transmission mice. synapses express neuronal synthase (nNOS) postsynaptically and NO receptors (NO-sensitive guanylyl cyclase) presynaptic terminals. We hypothesized that—similar...
The NKCC1 ion transporter contributes to the pathophysiology of common neurological disorders, but its function in microglia, main inflammatory cells brain, has remained unclear date. Therefore, we generated a novel transgenic mouse line which microglial was deleted. We show that shapes both baseline and reactive microglia morphology, process recruitment site injury, adaptation changes cellular volume cell-autonomous manner via regulating membrane conductance. In addition, deficiency results...
Nitric oxide (NO) plays an important role in synaptic plasticity as a retrograde messenger at glutamatergic synapses. Here we describe that, hippocampal pyramidal cells, neuronal nitric synthase (nNOS) is also associated with the postsynaptic active zones of GABAergic symmetrical synapses terminating on their somata, dendrites, and axon initial segments both mice rats. The NO receptor oxide-sensitive guanylyl cyclase (NOsGC) present brain two functional subunit compositions: α 1 β 2 ....
Abstract Acute brain slices represent a workhorse model for studying the central nervous system (CNS) from nanoscale events to complex circuits. While slice preparation inherently involves tissue damage, it is unclear how microglia, main immune cells and damage sensors of CNS react this injury shape neuronal activity ex vivo. To end, we investigated microglial phenotypes contribution network organization functioning in acute slices. We reveal time-dependent phenotype changes influenced by...
Abstract Septohippocampal cholinergic neurons play key roles in learning and memory processes, the generation of hippocampal theta rhythm. The range receptors for endogenous modulators expressed on these is unclear. Here we describe GABA B 1a/b receptor (GABA R) type 1 cannabinoid (CB expression rat septal [i.e. choline acetyltransferase (ChAT)‐positive] cells. Using double immunofluorescent staining, found that almost two‐thirds cells medial septum were R positive, had significantly larger...
GABA (gamma-aminobutyric-acid), the main inhibitory neurotransmitter in adult brain, exerts depolarizing (excitatory) actions during development and this GABAergic depolarization cooperates with NMDARs (N-methyl-D-aspartate receptors) to drive spontaneous synchronous activity (SSA) that is fundamentally important for developing neuronal networks. Although known assist activation of development, subcellular localization relative synapses still unknown. Here, we investigated distribution...
Early γ-aminobutyric acid mediated (GABAergic) synaptic transmission and correlated neuronal activity are fundamental to network formation; however, their regulation during early postnatal development is poorly understood. Nitric oxide (NO) an important retrograde messenger at glutamatergic synapses, it was recently shown play role also GABAergic synapses in the adult brain. The subcellular localization effect of this signaling pathway so far unexplored, but its disruption age known lead...
Abstract Genetic studies have linked the primate-specific gene locus G72 to development of schizophrenia and bipolar disorder. Transgenic mice carrying entire express mRNA in dentate gyrus (DG) entorhinal cortex, causing altered electrophysiological properties their connections. These transgenic exhibit behavioral alterations related psychiatric diseases, including cognitive deficits that can be reversed by treatment with N -acetylcysteine, which was also found effective human patients....
Abstract COVID-19 is associated with diverse neurological abnormalities, which predict poor outcome in patients. However, the mechanisms whereby infection-induced inflammation could affect complex neuropathologies are unclear. We hypothesized that microglia, resident immune cells of brain, centrally involved this process. To study this, we developed an autopsy platform allowing integration molecular anatomy-, protein- and mRNA data sets post-mortem mirror blocks brain peripheral organ...
Abstract Acute brain slices represent a “workhorse” model for studying the central nervous system (CNS) from nanoscale events to complex circuits. While slice preparation inherently involves tissue injury, it is unclear how microglia, main immune cells and damage sensors of CNS shape integrity ex vivo . To this end, we have studied mechanisms microglial phenotype changes contribution neuronal network organisation functioning in acute slices. Using novel ATP- reporter mouse line microglia...
Abstract The NKCC1 ion transporter contributes to the pathophysiology of common neurological disorders, but its function in microglia, main inflammatory cells brain, has remained unclear date. Therefore, we generated a novel transgenic mouse line which microglial was deleted. We show that shapes both baseline and reactive microglia morphology, process recruitment site injury, adaptation osmotic stress cell-autonomous manner via regulating membrane potential chloride fluxes. In addition,...