A5028782341- Neuroscience and Neuropharmacology Research
- Cannabis and Cannabinoid Research
- Sleep and Wakefulness Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurotransmitter Receptor Influence on Behavior
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience of respiration and sleep
- Polish Historical and Cultural Studies
- Pain Mechanisms and Treatments
- Receptor Mechanisms and Signaling
- GABA and Rice Research
- European and International Law Studies
- Local Governance and Planning
- Memory and Neural Mechanisms
- European Law and Migration
- Advanced Fluorescence Microscopy Techniques
- Hungarian Social, Economic and Educational Studies
- Photoreceptor and optogenetics research
- Adenosine and Purinergic Signaling
- Neural dynamics and brain function
- European Politics and Security
- Biochemical effects in animals
- Immune cells in cancer
- Prenatal Substance Exposure Effects
- Advanced Electron Microscopy Techniques and Applications
HUN-REN Institute of Experimental Medicine
2015-2025
Indiana University Bloomington
2020-2025
Hungarian Academy of Sciences
2011-2024
Indiana University
2022
Pázmány Péter Catholic University
2019
Koblenz University of Applied Sciences
2017
Weatherford College
2017
Karolinska Institutet
2007
University of Aberdeen
2007
Yale University
2007
The endogenous cannabinoids (endocannabinoids) are lipid molecules that may mediate retrograde signaling at central synapses and other forms of short-range neuronal communication. monoglyceride 2-arachidonoylglycerol (2-AG) meets several criteria an endocannabinoid substance: ( i ) it activates cannabinoid receptors; ii is produced by neurons in activity-dependent manner; iii rapidly eliminated. 2-AG inactivation only partially understood, but occur transport into cells enzymatic hydrolysis....
To understand the functional significance and mechanisms of action in CNS endogenous exogenous cannabinoids, it is crucial to identify neural elements that serve as structural substrate these actions. We used a recently developed antibody against CB1 cannabinoid receptor study this question hippocampal networks. Interneurons with features typical basket cells showed selective, intense staining for all subfields layers. Most them (85.6%) contained cholecystokinin (CCK), which corresponded...
Cannabinoids are the most popular illicit drugs used for recreational purposes worldwide. However, neurobiological substrate of their mood-altering capacity has not been elucidated so far. Here we report that CB1 cannabinoid receptors expressed at high levels in certain amygdala nuclei, especially lateral and basal but absent other nuclei (e.g., central nucleus medial nucleus). Expression protein was restricted to a distinct subpopulation GABAergic interneurons corresponding large...
Abstract Using a new antibody developed against the C‐terminus of cannabinoid receptor (CB1), immunostaining in hippocampus revealed additional axon terminals relative to pattern reported previously with an N‐terminus antibody. Due greater sensitivity this antibody, large proportion boutons dendritic layers displaying symmetrical (GABAergic) synapses were also strongly immunoreactive for CB1 receptors, as perisomatic inhibitory cells containing cholecystokinin. Asymmetrical (glutamatergic)...
Microglia are the main immune cells in brain and have roles homeostasis neurological diseases. Mechanisms underlying microglia-neuron communication remain elusive. Here, we identified an interaction site between neuronal cell bodies microglial processes mouse human brain. Somatic junctions a specialized nanoarchitecture optimized for purinergic signaling. Activity of mitochondria was linked with junction formation, which induced rapidly response to activation blocked by inhibition P2Y12...
The roles of endocannabinoid signaling during central nervous system development are unknown. We report that CB 1 cannabinoid receptors (CB Rs) enriched in the axonal growth cones γ-aminobutyric acid–containing (GABAergic) interneurons rodent cortex late gestation. Endocannabinoids trigger R internalization and elimination from filopodia induce chemorepulsion collapse these GABAergic by activating RhoA. Similarly, endocannabinoids diminish galvanotropism Xenopus laevis spinal neurons. These...
Endocannabinoids play central roles in retrograde signaling at a wide variety of synapses throughout the CNS. Although several molecular components endocannabinoid system have been identified recently, their precise location and contribution to synaptic is essentially unknown. Here we show, by using two independent riboprobes, that principal cell populations hippocampus express high levels diacylglycerol lipase α (DGL-α), enzyme involved generation 2-arachidonoyl-glycerol (2-AG)....
Endogenous cannabinoids (endocannabinoids) mediate retrograde signals for short- and long-term suppression of transmitter release at synapses striatal medium spiny (MS) neurons. An endocannabinoid, 2-arachidonoyl-glycerol (2-AG), is synthesized from diacylglycerol (DAG) after membrane depolarization Gq-coupled receptor activation. To understand 2-AG-mediated signaling in the striatum, we determined precise subcellular distributions synthetic enzyme 2-AG, DAG lipase-α (DAGLα), its upstream...
Fragile X syndrome, the most commonly known genetic cause of autism, is due to loss fragile mental retardation protein, which regulates signal transduction at metabotropic glutamate receptor-5 in brain. protein deletion mice enhances receptor-5-dependent long-term depression hippocampus and cerebellum. Here we show that a distinct type excitatory synapses ventral striatum prefrontal cortex, mediated by endocannabinoid 2-arachidonoyl-sn-glycerol, absent protein-null mice. In these mutants,...
Endocannabinoid-mediated retrograde signaling exerts powerful control over synaptic transmission in many brain areas. However, the neocortex, precise laminar, cellular, and subcellular localization of type 1 cannabinoid receptor (CB ) as well its function has been elusive. Here we combined multiple immunolabeling with whole-cell recordings to investigate morpho-functional characteristics rat somatosensory cortex. Immunostaining for CB revealed axonal somatic labeling striking layer...
GABAergic interneurons can pace the activity of principal cells and are thus critically involved in generation oscillatory synchronous network activity. The specific role various subpopulations, however, has remained elusive. This is part attributable to scarcity certain neurons difficulty identifying them slices obtained from brain regions which anatomical structures not readily recognizable live preparation. To facilitate functional analysis interneurons, we generated transgenic mice...
Endocannabinoid signaling is a key regulator of synaptic neurotransmission throughout the brain. Compelling evidence shows that its perturbation leads to development epileptic seizures, thus indicating endocannabinoids play an intrinsic protective role in suppressing pathologic neuronal excitability. To elucidate whether long-term reorganization endocannabinoid occurs patients, we performed comparative expression profiling along with quantitative electron microscopic analysis control...
Diminished synaptic inhibition in the spinal dorsal horn is a major contributor to chronic pain. Pathways that reduce inflammatory and neuropathic pain states have been identified, but central hyperalgesia diminished also occur absence of inflammation or neuropathy, solely triggered by intense nociceptive (C-fiber) input horn. We found endocannabinoids, produced upon strong stimulation, activated type 1 cannabinoid (CB1) receptors on inhibitory neurons release gamma-aminobutyric acid glycine...
The medial septum (MS) is an indispensable component of the subcortical network which synchronizes hippocampus at theta frequency during specific stages information processing. GABAergic neurons exhibiting highly regular firing coupled to hippocampal rhythm are thought form core MS rhythm-generating network. In recent studies hyperpolarization-activated, cyclic nucleotide-gated non-selective cation (HCN) channel was shown participate in synchronization septum. Here, we tested hypothesis that...