A5020590226- Neuroscience and Neuropharmacology Research
- Genetics and Neurodevelopmental Disorders
- Mitochondrial Function and Pathology
- Autism Spectrum Disorder Research
- Neurogenesis and neuroplasticity mechanisms
- Cellular transport and secretion
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Cannabis and Cannabinoid Research
- Lipid Membrane Structure and Behavior
- Tryptophan and brain disorders
- RNA regulation and disease
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Neurogenetic and Muscular Disorders Research
- Single-cell and spatial transcriptomics
- Neuroscience and Neural Engineering
- Cell Image Analysis Techniques
- GABA and Rice Research
- Ubiquitin and proteasome pathways
- Amino Acid Enzymes and Metabolism
- Nerve injury and regeneration
- Pancreatic function and diabetes
- RNA modifications and cancer
University of California, Berkeley
2022-2025
Berkeley College
2025
Albert Einstein College of Medicine
2016-2023
Long-lasting forms of postsynaptic plasticity commonly involve protein synthesis-dependent structural changes dendritic spines. However, the relationship between synthesis and presynaptic remains unclear. Here, we investigated in cannabinoid-receptor 1 (CB )-mediated long-term depression inhibitory transmission (iLTD), a form that involves protein-synthesis-dependent long-lasting reduction GABA release. We found CB -iLTD acute rat hippocampal slices was associated with changes. Using...
Fragile X syndrome (FXS) is a leading cause of inherited intellectual disability and autism. Whereas dysregulated RNA translation in Fmr1 knockout (KO) mice, model FXS, well studied, little known about aberrant transcription. Using single-molecule mRNA detection, we show that encoding the AMPAR subunit GluA2 (but not GluA1) elevated dendrites at transcription sites hippocampal neurons KO indicating We identify CPEB3, protein implicated memory consolidation, as an upstream effector critical...
Gene expression is tightly regulated by RNA-binding proteins (RBPs) to facilitate cell survival, differentiation, and migration. Previous reports have shown the importance of Insulin-like Growth Factor II mRNA-Binding Protein (IGF2BP1/IMP1/ZBP1) in regulating RNA fate, including localization, transport, translation. Here, we generated characterized a knockout mouse study RBP regulation. We report that IGF2BP1 essential for proper brain development neonatal survival. Specifically, these mice...
ABSTRACT Mouse whisker somatosensory cortex (wS1) is a major model system to study the experience-dependent plasticity of cortical neuron physiology, morphology, and sensory coding. However, role experience in regulating neuronal cell type development gene expression wS1 remains poorly understood. We assembled annotated transcriptomic atlas during postnatal comprising 45 molecularly distinct types that can be grouped into eight excitatory four inhibitory subclasses. Using this atlas, we...
Circuit dysfunction in autism may involve a failure of homeostatic plasticity. To test this, we studied parvalbumin (PV) interneurons which exhibit rapid plasticity intrinsic excitability following whisker deprivation mouse somatosensory cortex. Brief reduces PV by increasing Kv1 current to increase spike threshold. We found that is disrupted Tsc2 +/- and Fmr1 -/- models autism. In wildtype mice, elevates the transcription factor ER81 drives Kcna1 transcription, Kv1.1 protein axon initial...
Mouse whisker somatosensory cortex (wS1) is a major model system to study the experience-dependent plasticity of cortical neuron physiology, morphology, and sensory coding. However, role experience in regulating neuronal cell type development gene expression wS1 remains poorly understood. We assembled transcriptomic atlas during postnatal comprising 45 molecularly distinct types that can be grouped into eight excitatory four inhibitory subclasses. Between day (P) 12, onset active whisking,...
Presynaptic boutons in the mammalian brain are typically small and difficult to manipulate study. Here, we present a protocol applying HaloTag self-labeling technology detect de novo local protein synthesis intact presynaptic mossy fiber from acute mouse hippocampal slices. We describe stereotaxic injection of HaloTag-expressing virus into region interest, followed by slice preparation. then detail labeling HaloTag-fused image acquisition visualize labeled an circuit. For complete details on...
Summary Long-lasting forms of postsynaptic plasticity commonly involve protein synthesis-dependent structural changes dendritic spines. However, the relationship between synthesis and presynaptic remains unclear. Here, we investigated in cannabinoid-receptor 1 (CB )-mediated long-term depression inhibitory transmission (iLTD), a form that requires involves long-lasting reduction GABA release. We found CB -iLTD acute rat hippocampal slices was associated with changes. Using proteomics,...
SUMMARY Learning and memory critically rely on long-lasting, synapse-specific modifications. While postsynaptic forms of plasticity typically require local protein synthesis, whether how synthesis contributes to presynaptic changes remains unclear. Here, we examined the hippocampal mossy fiber (MF)-CA3 synapse which expresses both structural functional plasticity. We report that MF boutons synthesize locally contain ribosomes. Long-term potentiation MF-CA3 synaptic transmission (MF-LTP) was...
Learning and memory rely on long-lasting, synapse-specific modifications. While postsynaptic forms of plasticity typically require local protein synthesis, whether how synthesis contributes to presynaptic changes remains unclear. Here, we examined the hippocampal mossy fiber (MF)-CA3 synapse which expresses both structural functional contains Fragile X mental retardation (FMRP), an RNA-binding previously implicated in synthesis-dependent plasticity. We report that MF boutons contain...