A5063306362- Osteoarthritis Treatment and Mechanisms
- Cell Adhesion Molecules Research
- Proteoglycans and glycosaminoglycans research
- Connective tissue disorders research
- Cellular Mechanics and Interactions
- Mesenchymal stem cell research
- Tendon Structure and Treatment
- Protease and Inhibitor Mechanisms
- Knee injuries and reconstruction techniques
- Spine and Intervertebral Disc Pathology
- TGF-β signaling in diseases
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Animal Genetics and Reproduction
- Inflammatory mediators and NSAID effects
- Periodontal Regeneration and Treatments
- Bone Metabolism and Diseases
- Bone health and treatments
- RNA Interference and Gene Delivery
- Bone and Dental Protein Studies
- Tissue Engineering and Regenerative Medicine
- Muscle Physiology and Disorders
- Bone Tissue Engineering Materials
- Bone fractures and treatments
- Advanced Fiber Optic Sensors
Munich University of Applied Sciences
2015-2024
Ludwig-Maximilians-Universität München
2015-2024
LMU Klinikum
2016-2024
Urologische Klinik München
2021-2022
Max Planck Institute of Biochemistry
2002-2016
Max Planck Society
2002-2010
Lund University
1998-2003
Salk Institute for Biological Studies
2000
Agricultural Biotechnology Institute
1996-2000
The University of Melbourne
1998
Perlecan is a heparan sulfate proteoglycan that expressed in all basement membranes (BMs), cartilage, and several other mesenchymal tissues during development. binds growth factors interacts with various extracellular matrix proteins cell adhesion molecules. Homozygous mice null mutation the perlecan gene exhibit normal formation of BMs. However, BMs deteriorate regions increased mechanical stress such as contracting myocardium expanding brain vesicles showing crucial for maintaining BM...
Fibromodulin is a member of family connective tissue glycoproteins/proteoglycans containing leucine-rich repeat motifs. Several members this gene bind to fibrillar collagens and are believed function in the assembly collagen network tissues. Here we show that mice lacking functional fibromodulin exhibit an altered morphological phenotype tail tendon with fewer abnormal fiber bundles. In fibromodulin-null animals virtually all bundles disorganized have morphology. Also 10–20% heterozygous...
Cyclic guanosine 3′,5′-monophosphate (cGMP)-dependent protein kinases (cGKs) mediate cellular signaling induced by nitric oxide and cGMP. Mice deficient in the type II cGK were resistant to Escherichia coli STa, an enterotoxin that stimulates cGMP accumulation intestinal fluid secretion. The cGKII-deficient mice also developed dwarfism was caused a severe defect endochondral ossification at growth plates. These results indicate cGKII plays central role diverse physiological processes.
β1 integrins are highly expressed on chondrocytes, where they mediate adhesion to cartilage matrix proteins. To assess the functions of integrin during skeletogenesis, we inactivated β 1 gene in chondrocytes. We show here that these mutant mice develop a chondrodysplasia various severity. β1-deficient chondrocytes had an abnormal shape and failed arrange into columns growth plate. This is caused by lack motility, which turn loss collagen type II, reduced binding impaired spreading...
Various types of collagen have been identified as potential ligands for the two mammalian discoidin domain receptor tyrosine kinases, DDR1 and DDR2. Here, we used a recombinant fusion protein between extracellular alkaline phosphatase to detect specific binding sites during mouse development. Major DDR1-binding activity, indicative ligand expression, were found in skeletal bones, skin, urogenital tract. Ligand expression uterus implantation mammary gland pregnancy colocalized with receptor....
Collagen II is a fibril-forming collagen that mainly expressed in cartilage. II–deficient mice produce structurally abnormal cartilage lacks growth plates long bones, and as result these develop skeleton without endochondral bone formation. Here, we report Col2a1-null are unable to dismantle the notochord. This defect associated with inability intervertebral discs (IVDs). During normal embryogenesis, nucleus pulposus of future IVDs forms from regional expansion notochord, which...
A large number of functions have been demonstrated for tenascin-C by antibody perturbation assays and in vitro cell culture experiments. However, these results contrast sharply with the lack any apparent phenotype mice a genetic deletion tenascin-C. possible explanation would be expression some altered but functional mutant. We report generation an independent null mouse conclude that original knockout, which is genetically very similar to ours, also true null. As found previously, absence...
The generation of nitric oxide (NO) in penile erectile tissue and the subsequent elevation cyclic GMP (cGMP) levels are important for normal erection. Current treatments dysfunction elevate either cGMP by blocking degrading phosphodiesterase 5 or AMP (cAMP) intrapenile injection prostaglandin E1. molecular target targets role cAMP erection not known. Herein, we report that mice lacking cGMP-dependent kinase I (cGKI) have a very low ability to reproduce their corpora cavernosa fail relax on...
Although mesenchymal stem cells (MSCs) are the natural source for bone regeneration, exact mechanisms governing MSC crosstalk with collagen I have not yet been uncovered. Cell adhesion to is mostly mediated by three integrin receptors – α1β1, α2β1 and α11β1. Using human (hMSC), we show that α11 subunit exhibited highest basal expression levels but on osteogenic stimulation, both α2 integrins were significantly upregulated. To elucidate possible roles of collagen-binding integrins, applied...
The growth plate (GP) is a dynamic tissue driving bone elongation through chondrocyte proliferation, hypertrophy and matrix production. extracellular (ECM) the major determinant of GP biomechanical properties assumed to play pivotal role for geometry arrangement, thereby guiding proper morphogenesis elongation. To elucidate relationship between morphology biomechanics during cartilage morphogenesis, we have investigated age-dependent structural elastic proliferative zone murine by atomic...
Abstract Reconstruction of bone defects represents a serious issue for orthopaedic and maxillofacial surgeons, especially in extensive loss. Adipose-derived mesenchymal stem cells (ADSCs) with tri-calcium phosphates (TCP) are widely used regeneration facilitating the formation extracellular matrix to promote reparative osteogenesis. The present study assessed potential cell-scaffold constructs mandibular minipig model. Sixteen skeletally mature miniature pigs were divided into two groups:...
AbstractCartilage oligomeric matrix protein (COMP) belongs to the thrombospondin family and is a homopentamer primarily expressed in cartilage. Mutations COMP gene result autosomal dominant chondrodysplasias pseudoachondroplasia (PSACH) some types of multiple epiphyseal dysplasia (MED), which are characterized by mild severe short-limb dwarfism early-onset osteoarthritis. We have generated COMP-null mice study role vivo. These show no anatomical, histological, or ultrastructural...
Integrin alpha10beta1 is a collagen-binding integrin expressed on chondrocytes. In order to unravel the role of alpha10 during development, we generated mice carrying constitutive deletion gene. The mutant had normal lifespan and were fertile but developed growth retardation long bones. Analysis skeleton revealed defects in plate after birth characterized by disturbed columnar arrangement chondrocytes, abnormal chondrocyte shape reduced proliferation. Electron microscopy plates from newborn...
The fibroblast integrin alpha11beta1 is a key receptor for fibrillar collagens. To study the potential function of alpha11 in vivo, we generated null allele gene. Integrin alpha11(-/-) mice are viable and fertile but display dwarfism with increased mortality, most probably due to severely defective incisors. Mutant incisors characterized by disorganized periodontal ligaments, whereas molar ligaments appear normal. primary defect incisor ligament leads halted tooth eruption....
Lysyl hydroxylase 3 (LH3) is a multifunctional enzyme possessing lysyl (LH), hydroxylysyl galactosyltransferase (GT) and galactosylhydroxylysyl glucosyltransferase (GGT) activities in vitro. To investigate the vivo importance of LH3-catalyzed lysine hydroxylation hydroxylysine-linked glycosylations, three different LH3-manipulated mouse lines were generated. Mice with mutation that blocked only LH activity LH3 developed normally, but showed defects structure basement membrane collagen fibril...
Mast cells are implicated in the pathogenesis of inflammatory and autoimmune diseases. However, this notion based on studies mast cell-deficient mice is controversial. We therefore established an vivo model for hyperactive by specifically ablating NF-κB negative feedback regulator A20. While A20 deficiency did not affect cell degranulation, it resulted amplified pro-inflammatory responses downstream IgE/FcεRI, TLRs, IL-1R, IL-33R. As a consequence house dust mite- IL-33-driven lung...
Chondromodulin-I (CHM1) was identified recently as an angiogenesis inhibitor in cartilage. It is highly expressed the avascular zones of cartilage but absent late hypertrophic region, which invaded by blood vessels during enchondral ossification. Blast searches with C-terminal part CHM1 available databases led to identification human and mouse cDNAs encoding a new protein, Tendin, that shares high homology CHM1. Based on computer predictions, Tendin type II transmembrane protein containing...