A5101538448- Advanced Fluorescence Microscopy Techniques
- Cancer Research and Treatments
- Supramolecular Self-Assembly in Materials
- Genetics, Aging, and Longevity in Model Organisms
- Cell Image Analysis Techniques
- Transgenic Plants and Applications
- CRISPR and Genetic Engineering
- Spectroscopy Techniques in Biomedical and Chemical Research
- Neuroscience and Neuropharmacology Research
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Advanced Antenna and Metasurface Technologies
- Photoreceptor and optogenetics research
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Neuroscience and Neural Engineering
- Image Processing and 3D Reconstruction
- Advanced Proteomics Techniques and Applications
- Redox biology and oxidative stress
- Metamaterials and Metasurfaces Applications
- Sulfur Compounds in Biology
- Ga2O3 and related materials
- Melamine detection and toxicity
- bioluminescence and chemiluminescence research
- Plasmonic and Surface Plasmon Research
Westlake University
2021-2025
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2021-2025
Third Affiliated Hospital of Guangzhou Medical University
2024
Guangzhou Medical University
2024
Nanjing University of Posts and Telecommunications
2023-2024
Kurchatov Institute
2023
Shanghai Advanced Research Institute
2023
Moscow Institute of Physics and Technology
2023
Southern Medical University
2019-2022
Zhejiang University
2021
Stress granules (SGs) are discrete assemblies of stalled messenger ribonucleoprotein complexes (mRNPs) that form when eukaryotic cells encounter environmental stress. RNA-binding proteins (RBPs) mediate their condensation by recruiting populations mRNPs. However, the cellular and molecular mechanisms underlying role ubiquitin-associated protein 2-like (UBAP2L) in regulation SG dynamics remain elusive. Here, we show UBAP2L is required for both assembly disassembly. overexpression nucleated...
Abstract Spatially resolved proteomics is an emerging approach for mapping proteome heterogeneity of biological samples, however, it remains technically challenging due to the complexity tissue microsampling techniques and mass spectrometry analysis nanoscale specimen volumes. Here, we describe a spatially method based on combination expansion with spectrometry-based proteomics, which call Expansion Proteomics (ProteomEx). ProteomEx enables quantitative profiling spatial variability in...
Abstract Recent advancements in genetically encoded calcium indicators, particularly those based on green fluorescent proteins, have optimized their performance for monitoring neuronal activities a variety of model organisms. However, progress developing red-shifted GECIs, despite advantages over has been slower, resulting fewer options end-users. In this study, we explored topological inversion and soma-targeting strategies, which are complementary to conventional mutagenesis, re-engineer...
Genetically encoded voltage indicators (GEVIs) allow for the cell-type-specific real-time imaging of neuronal membrane potential dynamics, which is essential to understanding information processing at both cellular and circuit levels. Among GEVIs, near-infrared-shifted GEVIs offer faster kinetics, better tissue penetration, compatibility with optogenetic tools, enabling all-optical electrophysiology in complex biological contexts. In our previous work, we employed directed molecular...
Abstract Genetically encoded voltage indicators (GEVIs) have significantly advanced imaging, offering spatial details at cellular and subcellular levels not easily accessible with electrophysiology. In addition to fluorescence certain chemical bond vibrations are sensitive membrane potential changes, presenting an alternative imaging strategy; however, challenges in signal sensitivity specificity highlight the need develop vibrational spectroscopic GEVIs (vGEVIs) mammalian cells. To address...
Abstract Spectrally diverse fluorescent proteins (FPs) provide straightforward means for multiplexed imaging of biological systems. Among FPs fitting standard color channels, blue (BFPs) are characterized by lower brightness compared to other spectral counterparts. Furthermore, available BFPs were not systematically in cultured mammalian cells and common model organisms. Here we introduce a pair new BFPs, named Electra1 Electra2, developed through hierarchical screening bacterial using novel...
Abstract The optical chirality of metamaterials gives birth to distinct responses left circularly polarized (LCP) and right (RCP) light, leading asymmetric transmission circular dichroism (CD) light. In this work, meta-atom structure composed square semicircular combinations were designed, which exhibits extreme high ratio transmission, reflection CD values in blue light range. compare other reported structures, the can achieve better transmission. At wavelength 460 nm, incident LCP be...
Engineered light, oxygen, and voltage (LOV)-based proteins are able to fluoresce without oxygen requirement due the autocatalytic incorporation of exogenous flavin as a chromophore thus allowing for live cell imaging under hypoxic anaerobic conditions. They were also discovered have high sensitivity transition metal ions physiological derivatives. These properties make flavin-binding fluorescent (FPs) perspective platform biosensor development. However, brightness currently available FPs is...
In vivo imaging of model organisms is heavily reliant on fluorescent proteins with high intracellular brightness. Here we describe a practical method for rapid optimization via directed molecular evolution in cultured mammalian cells. Using this method, were able to perform screening large gene libraries containing up 2 × 10
Pterygia, caused by fibrovascular growth of conjunctiva, are a common ophthalmic disease. However, the molecular composition pterygium tissue has not been completely understood, and therefore aim this study is to investigate spectroscopic differences between normal human bulbar conjunctiva using confocal Raman system. The high signal-to-noise ratio spectra from were obtained technique without any sample preparation time detection required less than 3 min. Comparing two types tissue, there...
Abstract High brightness and photostability of StayGold make it a particularly attractive probe for long-term live cell imaging. However, its dimeric nature precludes application as fluorescent tag proteins. Here, we report the development X-ray structures monomeric variant (mStayGold), which preserves beneficial properties precursor while serving structural proteins membranes. We compare mStayGold to other state-of-art GFPs utilize super-resolution
Traditional LEDs emit light that exhibits incoherence and displays a Lambertian distribution. To achieve linearly polarized (LP) control the emission direction, variety of optical components are required to be stacked, which is unsuitable for compact applications results in low deflection efficiency. Here, we propose numerically simulate novel single-chip micro-resonant cavity LED (micro-RCLED) device generates directional LP by integrating continuous metasurface. This includes bilayer...
Potassium ion (K+) dynamics are vital for various biological processes. However, the limited availability of detection tools tracking intracellular and extracellular K+ has impeded a comprehensive understanding physiological roles in intact systems. In this study, we developed two novel red genetically encoded potassium indicators (RGEPOs), RGEPO1 RGEPO2, through combination directed evolution E. coli subsequent optimization mammalian cells. RGEPO1, targeted to membrane, localized cytoplasm,...
ABSTRACT Splicing factor proline‐glutamine rich (SFPQ), a multifunctional RNA‐binding protein (RBP), shows cytoplasmic colocalisation with stress granule (SG) markers; however, the causative relationship and mechanism underlying this coalescence of SFPQ aggregates SGs remain unclear. In study, we demonstrate that lacking its nuclear localisation sequence spontaneously forms abnormally incorporate immature RNA induce persistent SGs. mRNA profiling showed mislocalisation induced extensive...
In mammals, testis development is triggered by the expression of sex-determining Y-chromosome gene SRY to commit Sertoli cell (SC) fate at gonadal sex determination in fetus. Several genes have been identified be required promote pathway following activation (i.e., box 9 (SOX9)) an embryo; however, it largely remains unknown about and mechanisms involved stabilizing after birth throughout adulthood. Herein, we report postnatal males with SC-specific deletion Raptor demonstrated absence SC...