A5050205136- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Cell Image Analysis Techniques
- Photoacoustic and Ultrasonic Imaging
- Neuroinflammation and Neurodegeneration Mechanisms
- Photochromic and Fluorescence Chemistry
- Olfactory and Sensory Function Studies
- Receptor Mechanisms and Signaling
- Image Processing Techniques and Applications
- Neurobiology and Insect Physiology Research
- Nanoplatforms for cancer theranostics
- Memory and Neural Mechanisms
- SARS-CoV-2 detection and testing
- Tryptophan and brain disorders
- Renin-Angiotensin System Studies
- CCD and CMOS Imaging Sensors
- Adenosine and Purinergic Signaling
- Optical Imaging and Spectroscopy Techniques
- Advanced Optical Imaging Technologies
- Nicotinic Acetylcholine Receptors Study
- Sleep and Wakefulness Research
- Hungarian Social, Economic and Educational Studies
HUN-REN Institute of Experimental Medicine
2014-2024
Czech Academy of Sciences, Institute of Experimental Medicine
2022-2023
Institute of Cognitive Neuroscience and Psychology
2023
HUN-REN Research Centre for Natural Sciences
2023
Hungarian Research Network
2021
Hungarian Academy of Sciences
2012-2019
Microglia are the main immune cells of brain and contribute to common diseases. However, it is unclear how microglia influence neuronal activity survival in injured vivo. Here we develop a precisely controlled model injury induced by cerebral ischaemia combined with fast vivo two-photon calcium imaging selective microglial manipulation. We show that elimination leads striking, 60% increase infarct size, which reversed repopulation. Microglia-mediated protection includes reduction excitotoxic...
Abstract Extracting neuronal spiking activity from large-scale two-photon recordings remains challenging, especially in mammals vivo , where large noises often contaminate the signals. We propose a method, MLspike, which returns most likely spike train underlying measured calcium fluorescence. It relies on physiological model including baseline fluctuations and distinct nonlinearities for synthetic genetically encoded indicators. Model parameters can be either provided by user or estimated...
Individual cortical neurons can selectively respond to specific environmental features, such as visual motion or faces. How this relates the selectivity of presynaptic network across layers remains unclear. We used single-cell-initiated, monosynaptically restricted retrograde transsynaptic tracing with rabies viruses expressing GCaMP6s image, in vivo, motion-evoked activity individual layer 2/3 pyramidal and their networks mouse primary cortex. Neurons within each exhibited similar direction...
Neurotropic herpesviruses can establish lifelong infection in humans and contribute to severe diseases including encephalitis neurodegeneration. However, the mechanisms through which brain's immune system recognizes controls viral infections propagating across synaptically linked neuronal circuits have remained unclear. Using a well-established model of alphaherpesvirus that reaches brain exclusively via retrograde transsynaptic spread from periphery, vivo two-photon imaging combined with...
Highlights•3D DRIFT AO scanning can extend each point to 3D lines, surfaces, or volume elements•Retained spatial information is used for post hoc movement correction of signals•Chessboard; snake; multi-cube; multi-3D line; and multi-layer, multi-frame scanning•Improved excitation efficiency large with GECIsSummaryUnderstanding neural computation requires methods such as acousto-optical (AO) that simultaneously read out activity on both the somatic dendritic scales. increase measurement speed...
Neocortex is classically divided into distinct areas, each specializing in different function, but all could benefit from reinforcement feedback to inform and update local processing. Yet it remains elusive how global signals like reward punishment are represented cortical computations. Previously, we identified a neuron type, vasoactive intestinal polypeptide (VIP)-expressing interneurons, auditory cortex that recruited by behavioral reinforcers mediates disinhibitory control inhibiting...
Abstract Slow wave activity (SWA) is a characteristic brain oscillation in sleep and quiet wakefulness. Although the cell types contributing to SWA genesis are not yet identified, principal role of neurons emergence this essential cognitive mechanism has been questioned. To address possibility astrocytic involvement SWA, we used transgenic rat line expressing calcium sensitive fluorescent protein both astrocytes interneurons simultaneously imaged neuronal vivo . Here demonstrate, for first...
Abstract Infrared neural stimulation is a promising tool for stimulating the brain because it can be used to excite with high spatial precision without need of delivering or inserting any exogenous agent into tissue. Very few studies have explored its use in brain, as most investigations focused on sensory motor nerve stimulation. Using intravital calcium imaging genetically encoded indicator GCaMP6f, here we show that application infrared induces intracellular signals Layer 2/3 neurons...
Abstract Neuronal plasticity has been shown to be causally linked coincidence detection through dendritic spikes (dSpikes). We demonstrate the existence of SPW-R-associated, branch-specific, local dSpikes and their computational role in basal dendrites hippocampal PV+ interneurons awake animals. To measure entire arbor long thin during SPW-Rs, we used fast 3D acousto-optical imaging an eccentric deep-brain adapter ipsilateral field potential recording. The regenerative calcium spike started...
DNI-Glu proved to be several times more efficient, than MNI-Glu and other analogues.
In this paper, we present an additional, new cage-GABA compound, called 4-amino-1-(4′-dimethylaminoisopropoxy-5′,7′-dinitro-2′,3′-dihydro-indol-1-yl)-1-oxobutane-γ-aminobutyric acid (iDMPO-DNI-GABA), and currently, compound is the only photoreagent, which can be applied for GABA uncaging without experimental compromises. By a systematic theoretical design successful synthesis of several compounds, best reagent exhibits high two-photon efficiency within 700–760 nm range with excellent...
Spontaneous synchronous population activity (SPA) can be detected by electrophysiological methods in cortical slices of epileptic patients, maintained a physiological medium vitro. In order to gain additional spatial information about the network mechanisms involved SPA generation, we combined studies with two-photon imaging. Neocortical prepared from postoperative tissue and tumor patients were dual perfusion chamber incubation medium. was recorded 24-channel extracellular linear...
Abstract Background The organism-wide effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral infection are well studied, but little is known about the dynamics how spreads in time among or within cells due to scarcity suitable high-resolution experimental systems. It has been reported that SARS-CoV-2 pathways converge at calcium influx and subcellular distribution changes. Imaging combined with a proper staining technique an effective tool for studying calcium-related...
The advancements in targeted drug release and experimental neuroscience have amplified the scientific interest photolabile protecting groups (PPGs) photouncaging. growing need for detection of uncaging events has led to development reporters with fluorescence turn-on upon uncaging. In contrast, fluorescent tags turn-off properties been drastically underexplored, although there are applications where they would be sought after. this work, a rhodamine-based tag is developed signal following...
Abstract Due to technical roadblocks, it is unclear how visual circuits represent multiple features or behaviorally relevant representations are selected for long-term memory. Here we developed Moculus, a head-mounted virtual reality platform mice that covers the entire field, and allows binocular depth perception full immersion. This controllable environment, with three-dimensional (3D) corridors 3D objects, in combination acousto-optical imaging, affords rapid learning uncovering of...
Two-photon microscopy is the ideal tool to study how signals are processed in functional brain tissue. However, early raster scanning strategies were inadequate record fast 3D events like action potentials.The aim of authors was various neuronal activity patterns with high signal-to-noise ratio an optical manner.Authors developed new data acquisition methods and microscope hardware.Multiple Line Scanning enables experimenter select multiple regions interests, doing this not just increases...
Abstract Neural circuits support rapid visual learning. However, due to technical roadblocks, it is not known how represent multiple features or behaviorally relevant representations are selected for long-term memory. Here we developed Moculus, a head-mounted virtual reality platform mice that covers the entire field, and allows binocular depth perception full immersion. This controllable environment, combined with fast acousto-optical imaging, affords learning uncovering of novel circuit...
Functional mapping of the connectivity brain is an essential procedure for neurosurgeons aiming maximal resection a tumor, while minimizing damage to eloquent cortex. Being able delineate location critical cortical areas in individual patients allows surgeon preserve sensorimotor and cognitive functions. Several methods exist functional mapping, however, none are capable delivering fast, label free cortex with millimeter precision. In this work, we investigate potential use infrared neural...
Abstract The organism-wide effects of viral infection SARS-CoV-2 are well studied, but little is known about the dynamics how spreads in time among or within cells due to scarcity suitable high-resolution experimental systems. Two-photon (2P) imaging combined with a proper subcellular staining technique has been an effective tool for studying mechanisms at such resolutions and organelle levels. Herein, we report development novel calcium sensor molecule along 2P-technique identifying...