The temperature-dependent fluorescence property of Rhodamine B was used to measure changes in temperature at the cellular level induced by either infrared laser light exposure or high intensity, ultrashort pulsed electric fields. thermal impact these stimuli were demonstrated time and contrasted with change observed extracellular bath. method takes advantage sensitivity fluorescent dye which has a quantum yield linearly dependent on temperature. effects different temporal pulse applications...

Abstract Infrared neural stimulation is a promising tool for stimulating the brain because it can be used to excite with high spatial precision without need of delivering or inserting any exogenous agent into tissue. Very few studies have explored its use in brain, as most investigations focused on sensory motor nerve stimulation. Using intravital calcium imaging genetically encoded indicator GCaMP6f, here we show that application infrared induces intracellular signals Layer 2/3 neurons...

Electrical stimulation is a powerful tool for investigating and modulating brain activity, as well treating neurological disorders. However, understanding the precise effects of electrical on neural activity has been hindered by limitations in recording neuronal responses near stimulating electrode, such artifacts electrophysiology or obstruction field view imaging. In this study, we introduce novel device fabricated from conductive polymers that transparent therefore compatible with optical...

Significance This work reports a processing route to fabricate hydrogel films through their rapid and spontaneous growth in aqueous solutions. approach exploits the strong solvent depletion created at surface of polymer substrate swelling solution. By immersing substrates that swell associative solutions, we showed this local suction effect induces gelation films. Experiments simple model captured how kinetics process can be deliberately controlled by handy parameters. Other solutes easily...

The influence of infrared laser pulses on intracellular Ca 2+ signaling was investigated in neural cell lines with fluorescent live imaging. probe Fluo‐4 used to measure HT22 mouse hippocampal neurons and nonelectrically excitable U87 human glioblastoma cells exposed 50 500 ms at 1470 nm. Fluorescence recordings demonstrated that stimulation induced an instantaneous transient similar dose‐response characteristics (half‐maximal effective energy density EC around 58 J.cm −2 ). For both type...

Abstract Glioblastoma is a highly aggressive brain tumor, very invasive and thus difficult to eradicate with standard oncology therapies. Bioelectric treatments based on pulsed electric fields have proven be successful method treat cancerous tissues. However, they rely stiff electrodes, which cause acute chronic injuries, especially in soft tissues like the brain. Here we demonstrate feasibility of delivering flexible electronics using an ovo vascularized tumor model. We show fluorescence...

Insufficient bone anchoring is a major limitation of artificial substitutes for connective osteoarticular tissues. The use coatings containing osseoconductive ceramic particles one the actively explored strategies to improve osseointegration and strengthen bone-implant interface general tissue engineering. Our hypothesis that hydroxyapatite (HA) can be coated robustly on specific assemblies PVA hydrogel fibers potential ligament replacements. A simple dip-coating method described produce...

Infrared neural stimulation (INS) is an optical method, label-free, not cell-type specific, that can modulate activity with a high spatial resolution. Recent studies have shown it also trigger calcium signaling in non-excitable cells such as astrocytes. Astrocytes participate communication, and play key role many neurodegenerative diseases calcium-dependent way. As infrared triggers both astrocytes neurons, appears good technique to explore the influence of neuronal communication pathology....

<title>Abstract</title> Infrared neural stimulation (INS) emerges as a promising tool for stimulating the nervous system by its high spatial precision and absence of use exogenous agents into tissue, which led to first successful proof concept in human brain. While networks have been focal point INS research, this technique is also non cell type specific it triggers activity electrically excitable cells. Despite increasing interest, there remains demonstrate well defined simultaneous...

ABSTRACT Electrical stimulation is a powerful tool for investigating and modulating brain activity, as well treating neurological disorders. However, understanding the precise effects of electrical on neural activity has been hindered by limitations in recording neuronal responses near stimulating electrode, such artifacts electrophysiology or obstruction field view imaging. In this study, we introduce novel device fabricated from conductive polymers that transparent therefore compatible...

The primary method of treatment for patients suffering from drug-resistant focal-onset epilepsy is resective surgery, which adversely impacts neurocognitive function. Radio frequency (RF) ablation and laser are the methods with most promise, achieving seizure-free rates similar to resection but less negative impact on However, there remains a number concerns open technical questions about these two thermal ablation, ones: (1) heating; (2) hemorrhage bleeding; (3) poor directionality....

The use of infrared laser light (IRLL) for biomedical applications has gained momentum the potential in humans. IRLL presents some limitations due to dangerousness these radiations when exceeding safety thresholds depending on target tissue. This position paper describes step by a well-known technical method usually applied microfluidics but here first time measure temperature and heat evolution cell culture environment under excitation at 800 nm. measurement is based property Rhodamine B...

This paper presents a miniaturized four-electrode probe for measuring the conductivity of biological tissues at radio frequencies. The was developed to address two common challenges in tissue measurements frequencies: limited size sample compared sensing volume and electrode polarization effect. Measurement samples can be made by miniaturizing so is smaller sample. To issue polarization, PEDOT:PSS coating applied on electrodes reduce impedance electrodes, moving effect lower frequencies...

Functional mapping of the connectivity brain is an essential procedure for neurosurgeons aiming maximal resection a tumor, while minimizing damage to eloquent cortex. Being able delineate location critical cortical areas in individual patients allows surgeon preserve sensorimotor and cognitive functions. Several methods exist functional mapping, however, none are capable delivering fast, label free cortex with millimeter precision. In this work, we investigate potential use infrared neural...

Effective closed-loop neuromodulation relies on the acquisition of appropriate physiological control variables and delivery an stimulation signal. In particular, electroneurogram (ENG) data acquired from a set electrodes applied at surface nerve may be used as potential variable in this field. Improved electrode technologies processing methods are clearly needed context. work, we evaluated new technology based multichannel organic (OE) signal chain order to detect respiratory-related bursts...

Interest in the interaction between laser light and biological samples has gained momentum recent years, particularly neurobiology, where there is significant potential to stimulate neurons with infrared light. Despite reports showing application of for neurostimulation, underlying mechanism still unknown. The two main hypotheses are based on thermal or electrostatic mechanisms. Here, a novel optical method presented make temperature measurements human neural cells under excitation (λ=800nm)...