A5057378310- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- Drug Transport and Resistance Mechanisms
- Adenosine and Purinergic Signaling
- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Pediatric Hepatobiliary Diseases and Treatments
- Inhalation and Respiratory Drug Delivery
- Ion Channels and Receptors
- Magnesium in Health and Disease
- Trace Elements in Health
- Mesenchymal stem cell research
- Neuroscience and Neuropharmacology Research
- Bacterial biofilms and quorum sensing
- Bone and Dental Protein Studies
- Clinical Nutrition and Gastroenterology
- Liver Diseases and Immunity
- Tissue Engineering and Regenerative Medicine
- dental development and anomalies
- Wound Healing and Treatments
- Neonatal Health and Biochemistry
- Neuroscience of respiration and sleep
- Liver Disease Diagnosis and Treatment
- Renin-Angiotensin System Studies
- Advanced battery technologies research
Semmelweis University
2012-2024
University of Alabama at Birmingham
2003-2012
Medical University of Vienna
2008
University of Alabama
2003-2004
Hungarian Academy of Sciences
2003
University of Padua
1995-2002
University of Vienna
2000-2002
Azienda Ospedaliera di Padova
1998-2000
Vienna General Hospital
2000
We investigated the effects of bicarbonate on growth several different bacteria as well its biofilm formation and intracellular cAMP concentration in Pseudomonas aeruginosa. Biofilm was examined 96-well plates, with or without bicarbonate. The production measured by a commercial assay kit. found that NaHCO3 (100 mmol l-1) significantly inhibited, whereas NaCl did not influence planktonic bacteria. MIC MBC measurements indicated effect HCO3- is bacteriostatic rather than bactericidal....
Background: 3D printing is a rapidly developing technology in the healthcare industry and dentistry. Its application clearly shows that this area of digital dentistry has potential for everyday usage across all fields, including prosthodontics, orthodontics, maxillofacial surgery, oral implantology. However, despite gaining ground, there lack information about how specialists (dentists dental technicians) use additive technology. Our research group aimed to investigate impact social media on...
Primary biliary cirrhosis (PBC) is a disorder of unknown origin with autoimmune features. Recently, impaired secretion bicarbonate has been shown in patients PBC. Here we have investigated whether bile duct epithelial cells isolated from PBC exhibit defects transepithelial transport by analyzing the activities 2 ion exchangers, Cl − /HCO 3 anion exchanger (AE2) and Na + /H (NHE) cholangiocytes. AE2 NHE were studied basal conditions after stimulation cyclic adenosine monophosphate (cAMP)...
The absorption of water and ions (especially Na+ Cl−) is an important function colonic epithelial cells in both physiological pathophysiological conditions. Despite the comprehensive animal studies, there are only scarce available data on ion transporter activities normal inflamed human colon. In this study, 128 healthy controls 69 patients suffering from ulcerative colitis (UC) were involved. We investigated expressional functional characteristics Na+/H+ exchangers (NHE) 1–3, sodium channel...
Background/Aims: ATP-gated P2X4 purinergic receptors (P2X4Rs) are cation channels with important roles in diverse cell types. To date, lack of specific inhibitors has hampered investigations on P2X4Rs. Recently, the benzodiazepine derivative, 5-BDBD been proposed to selectively inhibit However, limited evidences currently available its inhibitory properties. Thus, we aimed characterize effects recombinant human Methods: We investigated ATP-induced intracellular Ca2+ signals and whole ion...
Purinergic receptor stimulation has potential therapeutic effects for cystic fibrosis (CF). Thus, we explored roles P2Y and P2X receptors in stably increasing [Ca2+]i human CF (IB3-1) non-CF (16HBE14o−) airway epithelial cells. Cytosolic Ca2+ was measured by fluorospectrometry using the fluorescent dye Fura-2/AM. Expression of (P2XR) subtypes assessed immunoblotting biotinylation. In IB3-1 cells, ATP other agonists caused only a transient increase derived from intracellular stores Na+-rich...
Cystic fibrosis (CF) is caused by defective cyclic AMP-dependent cystic transmembrane conductance regulator Cl- channels. Thus, CF epithelia fail to transport and water. A postulated therapeutic avenue in activation of alternative Ca2+-dependent We hypothesized that stimulation Ca2+ entry from the extracellular space could trigger a sustained signal activate Cytosolic [Ca2+]i was measured non-polarized human (IB3-1) non-CF (16HBE14o-) airway epithelial cells. Primary monolayers as well...
This study addresses the mechanisms by which a defect in CFTR impairs pancreatic duct bicarbonate secretion cystic fibrosis. We used control (PANC‐1) and CFTR‐deficient (CFPAC‐1; ΔF508 mutation) cell lines measured HCO 3 − extrusion rate of recovery intracellular pH after an alkaline load recorded whole membrane currents using patch clamp techniques. 1) In PANC‐1 cells, cAMP causes parallel activation Cl channels DIDS‐sensitive Na + ‐independent ‐ /HCO exchange, both effects being inhibited...
Biliary epithelial cells (cholangiocytes) modulate bile fluidity and alkalinity absorbing and/or secreting fluid electrolytes, particularly HCO 3 − Cl . Mechanisms responsible for transepithelial H + /HCO secretion in human cholangiocytes are largely unknown. Human isolated by enzymatic digestion immunomagnetic purification from normal liver tissue obtained reduced grafts used pediatric transplantation were cultured the presence of hepatocyte growth factor. Maintenance cholangiocyte...
In view of the occurrence hepatobiliary disorders in cystic fibrosis (CF) this study addresses role transmembrane conductance regulator (CFTR) and Ca(2+)-activated Cl(-) channels promoting HCO3- secretion bile ductular cells. Human cholangiocytes were isolated from control livers 1 patient with CF (DeltaF508/G542X mutations). Single channel whole cell currents analyzed by patch clamp techniques, was determined fluorometric analysis rate recovery intracellular pH following alkaline loading....
Clinical and experimental results with inhaled sodium bicarbonate as an adjuvant therapy in cystic fibrosis (CF) are promising due to its mucolytic bacteriostatic properties, but direct effect has not been studied on respiratory epithelial cells. Our aim was establish characterize co-culture models of human CF bronchial (CFBE) cell lines expressing a wild-type (WT) or mutant (deltaF508) transmembrane conductance regulator (CFTR) channel vascular endothelial cells investigate the effects...
Biliary epithelial cells (cholangiocytes) are responsible for rapid regulation of bile volume and alkalinity. Secretin other hormones raising intracellular cyclic adenosine monophosphate (cAMP) concentrations promote biliary HCO3 secretion by stimulating apical Cl- channels Cl-/HCO3- exchange (AE2). Cholangiocyte ion transport may also be stimulated locally acting mediators; example, 5'-triphosphate (ATP), a secretagogue that can released into the hepatocytes cholangiocytes, activates...
Transient transfection of epithelial cells with lipid reagents has been limited because toxicity and lack efficacy. In this study, we show that more recently developed lipids transfect nonpolarized human airway high efficacy efficiency little or no toxicity. Because success, hypothesized these may also allow transient polarized monolayers. A panel was tested for transfer the reporter gene luciferase ( LUC) into monolayers non-cystic fibrosis (non-CF) CF bronchial cells, MDCK cell monolayers,...
NMDA receptors in the prefrontal cortex (PFC) play a crucial role cognitive functions. Previous research has indicated that angiotensin II (Ang II) affects learning and memory. This study aimed to examine how Ang impacts receptor activity layer V pyramidal cells of rat PFC. Whole-cell patch-clamp experiments were performed brain slices 9-12-day-old rats. (30 μM) induced inward currents. (0.001-1 µM) significantly enhanced currents about 40% cells. enhancement was reversed by AT
Intrahepatic bile duct cells (cholangiocytes) play an important role in the secretion and alkalinization of bile. Both Na + /H exchange (NHE) Cl − /[Formula: see text]exchange (AE) contribute to these functions, but their functional distribution between apical basolateral membrane domains remains speculative. We have addressed this issue a normal rat cholangiocyte cell line (NRC-1), which maintains polarized markers. Gene expression AE NHE isoforms was studied by RT-PCR. For studies, were...
The aim of tissue engineering is to develop methods restore, maintain or improve functions. To imitate the fibrous structure native extracellular matrix, electrospinning technique widely used. However, dense packing fibers results in small pores and hereby inhibition cellular penetration. In this study, we used biocompatible biodegradable poly(aspartic acid) based hydrogel scaffolds enhance cell infiltration using ultrasonication (US). US can enlarge space between scaffold create a 3D on...
Despite extensive work in the field of glioblastoma research no significant increase survival rates for this devastating disease has been achieved. It is known that disturbance intracellular Ca(2+) ([Ca(2+)](i)) and pH (pH(i)) regulation could be involved tumor formation. The sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) a major regulator [Ca(2+)](i). We have investigated effect inhibition SERCA by thapsigargin (TG) on [Ca(2+)](i) pH(i) human primary multiforme (GBM) cells GBM cell...