ABSTRACT Although nitric oxide (NO) and reactive oxygen species (ROS) are essential signalling molecules required for mediation of abscisic acid (ABA)‐induced stomatal closure, it is not known whether these also mediate the ABA inhibition opening. In this study, we investigated role NO ROS in opening Vicia faba . induced both synthesis, scavenger reduced Exogenous hydrogen peroxide (H 2 O ) inhibited opening, indicating that involved process. An inhibitor synthase (NOS) reversed Either or...

Abstract Nitric oxide (NO) is a ubiquitous signaling molecule involved in diverse physiological processes, including plant senescence and stomatal closure. The NO cyclic GMP (cGMP) cascade the main pathway animals, but whether this operates cells, mechanisms of its action, remain unclear. Here, we assessed possibility that nitrated cGMP derivative 8-nitro-cGMP functions guard cell signaling. Mass spectrometry immunocytochemical analyses showed abscisic acid induced synthesis cells presence...

Plants are exposed to hydrogen sulfide (H2S) both exogenously, as it exists a pollutant gas in the environment, and endogenously, is synthesized cells. H2S has recently been found function gaseous signaling molecule, but its cascade remains unknown. Here, we examined H2S-mediated guard cell Arabidopsis. The donor GYY4137 (morpholin-4-ium-4-methoxyphenyl [morpholino] phosphinodithioate) induced stomatal closure, which peaked after 150 min at 1 µM or 90 10 100 µM. After reaching maximal...

Abstract Guard cells are indispensable for higher plants because they control gas exchange and water balance to maintain photosynthetic activity. The signaling processes that govern their movement controlled by several factors, such as abscisic acid (ABA), blue light, pathogen-associated molecular patterns (PAMPs), carbon dioxide. Herein, we demonstrated the amino glutamate (Glu), a well-known mammalian neurotransmitter, functions novel molecule in stomatal closure both Arabidopsis fava bean...

The effect of low temperature pretreatment buds or inflorescence on microspore culture for the production haploids Brassica rapa (syn. B.campestris) was examined. Incubation at 4°C 3 10 days before microspores induced efficient embryogenesis. Pretreatment flower more effective than that inflorescence. Prolonged up to 20 promoted embryo induction. Microspores in were examined developmental stage and after pretreatment. Buds with a petal length anther ratio about 0.5-0.7, which had late...

Reactive oxygen species (ROS) are ubiquitous signaling molecules involved in diverse physiological processes, including stomatal closure. Photosynthetic electron transport (PET) is the main source of ROS generation plants, but whether it functions guard cell remains unclear. Here, we assessed PET abscisic acid (ABA) cells. ABA-elicited were localized to chloroplasts Arabidopsis thaliana, Commelina benghalensis, and Vicia faba light abolished by inhibitors 3-(3, 4-dichlorophenyl)-1,...

In anther culture of Brassica campestris L., the yield pollen‐derived embryoids is greatly stimulated by a high‐temperature (35°C) tratment for first 1 to 3 days culture. We have inestigated effects treatment on mode pollen division in cultured anthers. Anthers containing late uninucleate were modified B5 medium. High‐temperature 24 h inhibited normal development and induced abnormal symmetrical division. Which step androgenesis. This was rarely observed developed vivo. anthers without...

Abstract Reactive oxygen species (ROS) are important signaling molecules in stomatal closure. In a previous report, we demonstrated that ROS generated through photosynthetic electron transport (PET) act as abscisic acid (ABA)‐induced However, the mechanism by which ABA induces generation PET remains unclear. Here, assessed possibility chloroplast K + /H EXCHANGE ANTIPORTER 3 (KEA3) functions ABA‐induced guard cells, resulting KEA3 localizes to thylakoid membrane and allows proton efflux from...

An abscisic acid (ABA)-insensitive Vicia faba mutant, fia (fava bean impaired in ABA-induced stomatal closure) had previously been isolated. In this study, it was investigated how FIA functions ABA signalling guard cells of faba. Unlike ABA, methyl jasmonate (MeJA), H2O2, and nitric oxide (NO) induced closure the mutant. did not induce production either reactive oxygen species or NO Moreover, suppress inward-rectifying K+ (Kin) currents activate ABA-activated protein kinase (AAPK) mutant...

Haploid melons were obtained from 12 cultivars when ovules pollinated with irradiated pollen cultured on either MS medium without plant growth substances or E20A 0.01mg/l IAA. All the plants haploid. The chromosome doubling occurred only in root tips of haploid plants, not other organs.

We isolated a mutant from Vicia faba L. cv. House Ryousai. It wilts easily under strong light and high temperature conditions, suggesting that its stomatal movement may be disturbed. determined responses of guard cells to some environmental stimuli. Mutant demonstrated an impaired ability respond ABA in 0.1 mM CaCl2 stomata did not close the presence up 1 ABA, whereas wild-type closed when exposed 10 µM ABA. Elevating external Ca2+ caused similar degree closure wild type mutant. A...

approximately 1.5-2.0mm wide, were collected.

湛水処理がサトイモ‘大吉’の生育，収量および蒸散に及ぼす影響について，ポット栽培において検討した．湛水処理開始後約120日目の‘大吉’の葉柄長は1.3～1.9倍に伸長し，地上部の生育が促進された．また，蒸散量も約2倍に増加した．収量は，畑地栽培用品種の‘大吉’は親芋重が1.3～4.4倍，分球芋重が1.6～3.7倍と増収し，湛水栽培用品種の‘田芋’同様，湛水栽培における適応性が高いことが示唆された．

We investigated the role of nitric oxide (NO) in ABA-inhibition stomatal opening Vicia faba L. different size dishes. When a large dish (9 cm diameter) was used, ABA induced NO synthesis and scavenger reduced opening. small (6 closure inhibited The able to reduce ABA-induced closure, but unable reverse Furthermore, not synthesized response ABA, indicating that is required for dish. These results indicated an NO-dependent NO-independent signaling pathway participate pathway. An major player...

An aseptic bioassay system to estimate allelopathy in asparagus calluses was established. Allelopathic activities of were measured according the callus packed cell volume (PCV) by means sandwich method, and remarkable inhibition acceptor plants observed addition over 0.1 mL PCV. When female male separately used for there no sexual difference allelopathic activity. To detect cells detail, we improved plant box method that substituted seedlings, serious with 0.35 PCV as donor material....