A5087395258- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Plant responses to water stress
- Genomics, phytochemicals, and oxidative stress
- Allelopathy and phytotoxic interactions
- Plant Parasitism and Resistance
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Phytochemicals and Antioxidant Activities
- Aluminum toxicity and tolerance in plants and animals
- Plant tissue culture and regeneration
- Alcohol Consumption and Health Effects
- Plant and Biological Electrophysiology Studies
- Calcium signaling and nucleotide metabolism
- Arsenic contamination and mitigation
- Plant Micronutrient Interactions and Effects
- GABA and Rice Research
- Plant Gene Expression Analysis
- Free Radicals and Antioxidants
- Polymer-Based Agricultural Enhancements
- Biochemical and biochemical processes
- Food Quality and Safety Studies
- Seed Germination and Physiology
- Tea Polyphenols and Effects
Okayama University
2016-2025
University of California, San Diego
2006-2018
Okayama Prefecture
2011
Okayama University of Science
2010
Abscisic acid (ABA) signal transduction has been proposed to utilize cytosolic Ca2+ in guard cell ion channel regulation. However, genetic mutants sensors that impair or plant signaling responses have not identified, and whether Ca2+-independent ABA mechanisms suffice for a full response remains unclear. Calcium-dependent protein kinases (CDPKs) contribute central plants. no Arabidopsis CDPK gene disruption mutant phenotype reported date, likely due overlapping redundancies CDPKs. Two...
Reactive oxygen species (ROS) mediate abscisic acid (ABA) signaling in guard cells. To dissect cell ABA-ROS genetically, a type-specific functional genomics approach was used to identify 2 MAPK genes, MPK9 and MPK12, which are preferentially highly expressed provide genetic evidence for their function, Arabidopsis single double TILLING mutants that carry deleterious point mutations these genes were isolated. RNAi-based gene-silencing plant lines, both silenced simultaneously, generated also....
Methyl jasmonate (MeJA) elicits stomatal closing similar to abscisic acid (ABA), but whether the two compounds use or different signaling mechanisms in guard cells remains be clarified. We investigated effects of MeJA and ABA on second messenger production ion channel activation wild-type Arabidopsis (Arabidopsis thaliana) MeJA-insensitive coronatine-insensitive 1 (coi1) mutants. The coi1 mutation impaired MeJA-induced not ABA-induced closing. as well induced reactive oxygen species (ROS)...
ABSTRACT Salicylic acid (SA), a ubiquitous phenolic phytohormone, is involved in many plant physiological processes including stomatal movement. We analysed SA‐induced closure, production of reactive oxygen species (ROS) and nitric oxide (NO), cytosolic calcium ion ([Ca 2+ ] cyt ) oscillations inward‐rectifying potassium (K + channel activity Arabidopsis . closure was inhibited by pre‐treatment with catalase (CAT) superoxide dismutase (SOD), suggesting the involvement extracellular ROS. A...
Abstract Abiotic stresses, including drought and salinity, trigger a complex osmotic-stress abscisic acid (ABA) signal transduction network. The core ABA signalling components are snf1-related protein kinase2s (SnRK2s), which activated by ABA-triggered inhibition of type-2C protein-phosphatases (PP2Cs). SnRK2 kinases also rapid, largely unknown, ABA-independent pathway. Here, through combination redundancy-circumventing genetic screen biochemical analyses, we have identified...
A central question is how specificity in cellular responses to the eukaryotic second messenger Ca2+ achieved. Plant guard cells, that form stomatal pores for gas exchange, provide a powerful system depth investigation of Ca2+-signaling plants. In intact abscisic acid (ABA) enhances (primes) Ca2+-sensitivity downstream signaling events result activation S-type anion channels during closure, providing mechanism Ca2+-signaling. However, underlying genetic and biochemical mechanisms remain...
In this study, we examined the involvement of endogenous abscisic acid (ABA) in methyl jasmonate (MeJA)-induced stomatal closure using an inhibitor ABA biosynthesis, fluridon (FLU), and ABA-deficient Arabidopsis (Arabidopsis thaliana) mutant, aba2-2. We found that pretreatment with FLU inhibited MeJA-induced but not ABA-induced wild-type plants. The aba2-2 mutation impaired closure. also investigated effects on cytosolic free calcium concentration ([Ca(2+)](cyt)) guard cells a...
Plant stomata limit both carbon dioxide uptake and water loss; hence, stomatal aperture is carefully set as the environment fluctuates. Aperture area known to be regulated in part by ion transport, but few of transporters have been characterized. Here we report that AtALMT12 (At4g17970), a homolog aluminum-activated malate transporter (ALMT) wheat, expressed guard cells Arabidopsis thaliana. Loss-of-function mutations impair closure induced ABA, calcium darkness, do not abolish either...
Abstract Previous studies have demonstrated that methyl jasmonate (MeJA) induces stomatal closure dependent on change of cytosolic free calcium concentration in guard cells. However, these molecular mechanisms intracellular Ca2+ signal perception remain unknown. Calcium-dependent protein kinases (CDPKs) function as transducers various plant physiological processes. It has been reported four Arabidopsis (Arabidopsis thaliana) CDPKs, CPK3, CPK6, CPK4, and CPK11, are involved abscisic acid...
The phenolic hormone salicylic acid (SA) induces stomatal closure. It has been suggested that SA signaling is integrated with abscisic (ABA) in guard cells, but the integration mechanism remains unclear. Ca2+-independent protein kinase Open Stomata1 (OST1) and Ca2+-dependent kinases (CPKs) are key for ABA-induced activation of slow-type anion channel SLAC1 Here, we show SA-induced closure impaired CPK disruption mutant cpk3-2 cpk6-1 not OST1 ost1-3. We also found phosphorylation sites ABA...
Stomata regulate gas exchange between plants and atmosphere by integrating opening closing signals. open in response to low CO2 concentrations maximize photosynthesis the light; however, mechanisms that coordinate stomatal conductance have yet be identified. Here we identify characterize CBC1/2 (CONVERGENCE OF BLUE LIGHT (BL) AND 1/2), two kinases link BL, a major component of photosynthetically active radiation (PAR), signals from guard cells. CBC1/CBC2 redundantly stimulate inhibition...
Abstract Cytosolic Ca2+ in guard cells plays an important role stomatal movement responses to environmental stimuli. These cytosolic increases result from influx through Ca2+-permeable channels the plasma membrane and release intracellular organelles cells. However, genes encoding defined channel activity remain unknown and, with some exceptions, largely higher plant Here, we report identification of two Arabidopsis (Arabidopsis thaliana) cation genes, CNGC5 CNGC6, that are highly expressed...
Stomatal pore apertures are narrowing globally due to the continuing rise in atmospheric [CO2]. CO2 elevation and plant hormone abscisic acid (ABA) both induce rapid stomatal closure. However, underlying signal transduction mechanisms for CO2/ABA interaction remain unclear. Two models have been considered: (i) enhances ABA concentrations and/or early signaling guard cells closure (ii) merges with at OST1/SnRK2.6 protein kinase activation. Here we use genetics, ABA-reporter imaging,...
Significance Fungal disease is a major threat to agriculture and consequently human nutrition. Many pathogenic fungi penetrate through stomata. This study reveals that chitin oligosaccharide (CTOS) from fungal cell wall induces stomatal closure its receptor CERK1, Ca 2+ , S-type anion channel SLAC1, which would prevent penetration. It also shown conversion of CTOS chitosan (CSOS) possible strategy circumvent immunity. At higher concentration, guard cells perceive CSOS, resulting in death,...
Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects, pathogens herbivores. In this study, we report that exogenously applied allyl isothiocyanate (AITC) induced stomatal closure Arabidopsis via production reactive oxygen species (ROS) nitric oxide (NO), elevation cytosolic Ca(2+) . AITC-induced closures were partially inhibited by an inhibitor NADPH oxidase completely glutathione monoethyl ester (GSHmee). ROS examined...
Abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure are accompanied by cytosolic alkalization in guard cells. However, it remains to be clarified how the functions not only ABA signaling but also MeJA. We investigated cells during ABA-, MeJA- Ca2+-induced of wild type, abi1-1, abi2-1, ost1-2 coi1 using a pH-sensitive fluorescent dye, BCECF-AM. induced wild-type whereas MeJA elicited coi1. Neither nor abi1-1 abi2-1 Exogenous Ca2+ wild-type, plants. An agent acidify...
Abscisic acid (ABA) induces production of reactive oxygen species (ROS) and nitric oxide (NO), elevation the cytosolic free calcium ion concentration ([Ca2+]cyt) pH (pHcyt), activation S-type anion channels in guard cells, causing stomatal closure. To investigate whether Arabidopsis Two pore channel 1 (AtTPC1) that encodes slow vacuolar (SV) is involved closure, we examined movements mobilization second messengers attpc1-2 loss-of-function mutant response to ABA, methyl jasmonate (MeJA)...
The plant hormone abscisic acid (ABA) controls growth and development regulates water status through an established signaling pathway. In the presence of ABA, pyrabactin resistance/regulatory component ABA receptor proteins inhibit type 2C protein phosphatases (PP2Cs). This, in turn, enables activation Sucrose Nonfermenting1-Related Protein Kinases2 (SnRK2). Open Stomata1 (OST1)/SnRK2.6/SRK2E is a major SnRK2-type kinase responsible for mediating responses. Arabidopsis (Arabidopsis thaliana)...