A5024769340- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Glycosylation and Glycoproteins Research
- Biochemical and Structural Characterization
- Legume Nitrogen Fixing Symbiosis
- Pharmaceutical Practices and Patient Outcomes
- Peptidase Inhibition and Analysis
- Synthetic Organic Chemistry Methods
- Marine Sponges and Natural Products
- Synthesis and Characterization of Heterocyclic Compounds
- Calcium signaling and nucleotide metabolism
- Plant Parasitism and Resistance
- Microbial Community Ecology and Physiology
- Health Systems, Economic Evaluations, Quality of Life
- Plant-Microbe Interactions and Immunity
- Cell Adhesion Molecules Research
- Metabolism and Genetic Disorders
- Angiogenesis and VEGF in Cancer
- Polysaccharides and Plant Cell Walls
- Chronic Disease Management Strategies
- Effects and risks of endocrine disrupting chemicals
Ludwig-Maximilians-Universität München
2021-2024
Urologische Klinik München
2024
University of Zurich
2021-2022
National Association of Statutory Health Insurance Physicians
2021
Institute of Plant Biology
2021
Institute of Plant Molecular Biology
2021
Conservatory and Botanical Garden of the City of Geneva
2015-2020
University of Geneva
2015-2020
University of California, San Diego
2011-2018
Florida International University
2016
The plant hormone abscisic acid (ABA) is produced in response to abiotic stresses and mediates stomatal closure drought via recently identified ABA receptors (pyrabactin resistance/regulatory component of receptor; PYR/RCAR). SLAC1 encodes a central guard cell S-type anion channel that ABA-induced closure. Coexpression the calcium-dependent protein kinase 21 (CPK21), CPK23, or Open Stomata 1 (OST1) activates currents. However, reconstitution activation any ion has not yet been attained....
A central question is how specificity in cellular responses to the eukaryotic second messenger Ca2+ achieved. Plant guard cells, that form stomatal pores for gas exchange, provide a powerful system depth investigation of Ca2+-signaling plants. In intact abscisic acid (ABA) enhances (primes) Ca2+-sensitivity downstream signaling events result activation S-type anion channels during closure, providing mechanism Ca2+-signaling. However, underlying genetic and biochemical mechanisms remain...
Plants constantly renew during their life cycle and thus require to shed senescent damaged organs. Floral abscission is controlled by the leucine-rich repeat receptor kinase (LRR-RK) HAESA peptide hormone IDA. It unknown how expression of IDA in zone leads activation. Here we show that sensed directly ectodomain. Crystal structures complex with reveal a binding pocket accommodates an active dodecamer peptide. A central hydroxyproline residue anchors receptor. The co-receptor SERK1, positive...
Abstract Large protein families are a prominent feature of plant genomes and their size variation is key element for adaptation. However, gene genome duplications pose difficulties functional characterization translational research. Here we infer the evolutionary history DOMAIN OF UNKNOWN FUNCTION (DUF) 26-containing proteins. The DUF26 emerged in secreted Domain rearrangements led to appearance CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASES (CRKs) PLASMODESMATA-LOCALIZED PROTEINS (PDLPs). land...
Abstract Arabidopsis root development is orchestrated by signaling pathways that consist of different CLAVATA 3/ EMBRYO SURROUNDING REGION ( CLE ) peptide ligands and their cognate CLV BARELY ANY MERISTEM BAM receptors. How where peptides trigger specific morphological or physiological changes in the poorly understood. Here, we report receptor‐like protein 2 2) pseudokinase CORYNE CRN are necessary to fully sense root‐active peptides. We uncover 3 as 45 receptor biochemically map its binding...
Plants use leucine-rich repeat receptor kinases (LRR-RKs) to sense sequence diverse peptide hormones at the cell surface. A 3.0-Å crystal structure of LRR-RK GSO1/SGN3 regulating Casparian strip formation in endodermis reveals a large spiral-shaped ectodomain. The domain provides binding platform for 21 amino acid CIF ligands, which are tyrosine sulfated by tyrosylprotein sulfotransferase TPST/SGN2. harbors pocket sulfotyrosine and makes extended backbone interactions with CIF2. Quantitative...
The plant hormone abscisic acid (ABA) controls growth and development regulates water status through an established signaling pathway. In the presence of ABA, pyrabactin resistance/regulatory component ABA receptor proteins inhibit type 2C protein phosphatases (PP2Cs). This, in turn, enables activation Sucrose Nonfermenting1-Related Protein Kinases2 (SnRK2). Open Stomata1 (OST1)/SnRK2.6/SRK2E is a major SnRK2-type kinase responsible for mediating responses. Arabidopsis (Arabidopsis thaliana)...
ABSTRACT CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptides are secreted endogenous plant ligands that sensed by receptor kinases (RKs) to convey environmental and developmental inputs. Typically, this involves an RK with narrow ligand specificity signals together a more promiscuous co-receptor. For most CLEs, biologically relevant (co-)receptors unknown. The dimer of the receptor-like protein CLAVATA 2 (CLV2) pseudokinase CORYNE (CRN) conditions perception so-called root-active CLE peptides,...
Plant genomes encode hundreds of secreted peptides; however, relatively few have been characterised. We report here an uncharacterised, stress-induced family plant signalling peptides, which we call CTNIPs. Based on the role common co-receptor BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1) in CTNIP-induced responses, identified Arabidopsis thaliana orphan receptor kinase HAESA-LIKE 3 (HSL3) as CTNIP via a proteomics approach. CTNIP-binding, ligand-triggered complex formation with...
Bacteria can trigger protein mistranslation to survive stress conditions. Mitochondria and plastids evolved from bacteria therefore also use prokaryotic-type expression machineries synthesize proteins. However, fungi animal mitochondria are highly sensitive mistranslation, which for instance manifests in lethal mitochondrial cardiomyopathy disorder. The response plant cells is unknown. Glutaminyl-transferRNAs (Gln-tRNAGln) of bacteria, mitochondria, synthesized indirectly. Initially, tRNAGln...
Abscisic acid (ABA) plays essential roles in plant development and responses to environmental stress. ABA induces subcellular translocation degradation of the guanine nucleotide exchange factor RopGEF1, thus facilitating core signal transduction. However, underlying mechanisms for ABA-triggered RopGEF1 trafficking/degradation remain unknown. Studies have revealed that RopGEFs associate with receptor-like kinases convey developmental signals small ROP GTPases. how activities are modulated is...
An effective total synthesis of (−)-callystatin A (1), member the leptomycin family antibiotics, has been achieved. The features Evans extended aldol methodology to construct northern polypropionate subunit and two separate Julia olefinations assemble conjugated dienes. proceeded in 2.3% overall yield with longest linear sequence 15 steps.
The cFMS proto-oncogene encodes for the colony-stimulating factor-1 receptor, a receptor-tyrosine kinase responsible differentiation and maturation of certain macrophages. Upon binding its ligand autophosphorylates, dimerizes, induces phosphorylation downstream targets. We report novel crystal structure unphosphorylated in complex with two members different classes drug-like protein inhibitors. exhibits typical bi-lobal fold, activation loop DFG motif are found to be canonical inactive...
2-Hydroxy-4,6-diamino-[1,3,5]triazines are described which a novel class of potent inhibitors the VEGF-R2 (flk-1/KDR) tyrosine kinase. 4-(Benzothiazol-6-ylamino)-6-(benzyl-isopropyl-amino)-[1,3,5]triazin-2-ol (14d) exhibited low nanomolar potency in vitro enzyme inhibition assay (IC50 = 18 nM) and submicromolar inhibitory activity KDR-induced MAP kinase autophosphorylation HUVEC cells 280 nM), also demonstrated good selectivity against panel growth factor receptor kinases. Further, 14d...
In nature plants constantly experience changes in light intensities. Low illumination limits photosynthesis and growth. However, also high intensities are a threat to as the photosynthetic machinery gets damaged when incoming energy surpasses capacity of photochemistry. One limitation photochemistry is constant resupply stromal electron (e-) acceptors, mainly NADP. NADP reduced at acceptor-side photosystem I. The resulting NADPH utilized by Calvin-Benson-Bassham cycle (CBBC) malate valve...
Abstract Objective Formative evaluation of the implementation process for a digitally supported intervention in polypharmacy Germany. Qualitative research was conducted within cluster randomized controlled trial (C-RCT). It focused on understanding how influences behavior-related outcomes prescription and medication review process. Methods/setting Twenty-seven general practitioners (GPs) were included study two groups C-RCT, intervention, wait list control group. Behavior-related...
Abstract Plants use leucine-rich repeat receptor kinases (LRR-RKs) to sense sequence diverse peptide hormones at the cell surface. A 3.0 Å crystal structure of LRR-RK GSO1/SGN3 regulating Casparian strip formation in endodermis reveals a large spiral-shaped ectodomain. The domain provides binding platform for 21 amino-acid CIF ligands, which are tyrosine sulfated by tyrosylprotein sulfotransferase TPST/SGN2. harbors pocket sulfotyrosine and makes extended backbone interactions with CIF2....
Abstract Large protein families are a prominent feature of plant genomes and their size variation is key element for adaptation in plants. Here we infer the evolutionary history representative family, DOMAIN OF UNKNOWN FUNCTION (DUF) 26-containing proteins. The DUF26 first appeared secreted Domain duplications rearrangements led to emergence CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASES (CRKs) PLASMODESMATA-LOCALIZED PROTEINS (PDLPs). While itself specific land plants, structural analyses...