A5053143384- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant Pathogenic Bacteria Studies
- Plant Stress Responses and Tolerance
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Plant Parasitism and Resistance
- Plant biochemistry and biosynthesis
- Plant Reproductive Biology
- Genetic diversity and population structure
- Insect Resistance and Genetics
- Enzyme Production and Characterization
- Gene expression and cancer classification
- Microbial Metabolic Engineering and Bioproduction
- Nematode management and characterization studies
- Bioenergy crop production and management
- Algal biology and biofuel production
- Plant Physiology and Cultivation Studies
- Microbial Metabolites in Food Biotechnology
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Botanical Studies and Applications
- Signaling Pathways in Disease
- Genomics, phytochemicals, and oxidative stress
Nanjing Medical University
2025
Huaian First People’s Hospital
2025
Nanjing Forestry University
2025
Huanghuai University
2024
Chinese Academy of Agricultural Sciences
2010-2024
Gregor Mendel Institute of Molecular Plant Biology
2024
Austrian Academy of Sciences
2024
University of Lausanne
2019-2024
Qingdao Agricultural University
2020-2024
Zhengzhou University
2024
Cutaneous squamous-cell carcinomas and keratoacanthomas are common findings in patients treated with BRAF inhibitors.
Cytoplasmic plant immune receptors recognize specific pathogen effector proteins and initiate effector-triggered immunity. In Arabidopsis, the RPS4 RRS1 are both required to activate defense three different pathogens. We show that physically associate. Crystal structures of N-terminal Toll-interleukin-1 receptor/resistance (TIR) domains RRS1, individually as a heterodimeric complex (respectively at 2.05, 1.75, 2.65 angstrom resolution), reveal conserved TIR/TIR interaction interface. TIR...
Accurate prediction of fault prone modules (a module is equivalent to a C function or C+ + method) in software development process enables effective detection and identification defects. Such models are especially beneficial for large-scale systems, where verification experts need focus their attention resources problem areas the system under development. This paper presents novel methodology predicting modules, based on random forests. Random forests an extension decision tree learning....
Plant immunity requires recognition of pathogen effectors by intracellular NB-LRR immune receptors encoded Resistance (R) genes. Most R proteins recognize a specific effector, but some function in pairs that multiple effectors. Arabidopsis thaliana TIR-NB-LRR RRS1-R and RPS4 together two bacterial effectors, AvrRps4 from Pseudomonas syringae PopP2 Ralstonia solanacearum. However, AvrRps4, not PopP2, is recognized rrs1/rps4 mutants. We reveal an gene pair resembles linked to RRS1/RPS4,...
Plant NLR (Nucleotide-binding domain and Leucine-rich Repeat) immune receptor proteins are encoded by Resistance (R) genes confer specific resistance to pathogen races that carry the corresponding recognized effectors. Some function in pairs, forming complexes for perception of We show here Arabidopsis RPS4 RRS1 both required make an authentic complex. Over-expression tobacco or results constitutive defense activation; this phenotype is suppressed presence RRS1. protein co-immunoprecipitates...
Plants use leucine-rich repeat receptor kinases (LRR-RKs) to sense sequence diverse peptide hormones at the cell surface. A 3.0-Å crystal structure of LRR-RK GSO1/SGN3 regulating Casparian strip formation in endodermis reveals a large spiral-shaped ectodomain. The domain provides binding platform for 21 amino acid CIF ligands, which are tyrosine sulfated by tyrosylprotein sulfotransferase TPST/SGN2. harbors pocket sulfotyrosine and makes extended backbone interactions with CIF2. Quantitative...
Significance Plants and animals carry intracellular nucleotide-binding leucine-rich repeat (NLR) immune receptors. How NLR receptors activate defense on perceiving pathogen molecules is poorly understood, especially in plants. Some NLRs function pairs, with one carrying a domain that mimics effector target. Effector action this activates the second “helper” NLR. In Arabidopsis RPS4 RRS1 pair, carries WRKY transcription factor targeted by bacterial effectors AvrRps4 PopP2. We monitored...
Significance Animal NLRs form wheel-like structures called inflammasomes upon perception of pathogen-associated molecules. The induced proximity the signaling domains at center wheel is hypothesized to recruit caspases for first step immune signal transduction. We expressed a plant-animal NLR fusion demonstrate that TIR from plant sufficient activate signaling. This demonstrates signaling-competent inflammasome can be formed known, minimal components. intrinsic NADase activity TIRs necessary...
New methodologies for surveillance and identification of Mycobacterium tuberculosis are required to stem the spread disease worldwide. In addition, ability discriminate mycobacteria at strain level may be important contact or source case investigations. To this end, we developing MALDI-TOF MS methods M. in culture. report, describe application MS, as well statistical analysis including linear discriminant random forest analysis, 16 medically relevant strains from four species mycobacteria,...
Recently, expression profiling of breast carcinomas has revealed gene signatures that predict clinical outcome, and discerned prognostically relevant cancer subtypes. Measurement the degree genomic instability provides a very similar stratification prognostic groups. We therefore hypothesized these features are linked. used 48 specimens profoundly differed in their identified set 12 genes defines 2 The biological significance this was established through survival prediction published...
Eggplant (Solanum melongena L.) and turkey berry (S. torvum Sw.), a wild ally of eggplant with promising multi-disease resistance traits, are great economic, medicinal genetic importance, but genomic resources for these species lacking. In the present study, we sequenced transcriptomes to accelerate research on two non-model species. We built comprehensive, high-quality de novo transcriptome assemblies Leptostemonum clade Solanum from short-read RNA-Sequencing data. obtained 34,174 unigenes...
In this study, the disease resistance gene PlWRKY65 was isolated from leaves of Paeonia lactiflora and analyzed by bioinformatics methods, localization encoded protein explored. Quantitative real-time PCR (qRT-PCR) also used to explore response Alternaria tenuissima. The results showed that sequence contained multiple cis-acting elements involved in hormone signaling molecules belonging IIe subgroup WRKY family, proteins were located nucleus. has a positive regulatory effect on A. tenuissima...
Abstract Purpose: Accurate prediction of an individual patient's drug response is important prerequisite personalized medicine. Recent pharmacogenomics research in chemosensitivity has studied the gene-drug correlation based on transcriptional profiling. However, proteomic profiling will more directly solve current functional and pharmacologic problems. We sought to determine whether signatures untreated cells were sufficient for response. Experimental Design: In this study, a machine...
Plant nucleotide-binding domain, leucine-rich repeat receptor (NLR) proteins play important roles in recognition of pathogen-derived effectors. However, the mechanism by which plant NLRs activate immunity is still largely unknown. The paired Arabidopsis RRS1-R and RPS4, that confer bacterial effectors AvrRps4 PopP2, are well studied, but how RRS1/RPS4 complex activates early immediate downstream responses upon effector detection poorly understood. To study without influence cell surface...
The regulation of cytoplasmic Ca2+ is crucial for various cellular processes. Here, we examined the levels in living fission yeast cells by a highly sensitive bioluminescence resonance energy transfer-based assay using GFP-aequorin fusion protein linked 19 amino acid. We monitored level and its change caused extracellular stimulants such as CaCl2 or NaCl plus FK506 (calcineurin inhibitor). found that extracellularly added dose-dependent increase resulted burst-like peak. overexpression two...
Abstract Populus wulianensis is an endangered species endemic to Shandong Province, China. Despite the economic and ornamental value of this species, few genomics genetic studies have been performed. In study, we performed a relevant analysis full-length transcriptome sequencing data P . obtained expressed sequence tag (EST)-simple repeat (SSR) markers with polymorphisms that can be used for further research. total, 8.18 Gb (3,521,665) clean reads average GC content 42.12% were obtained....
Perillaldehyde (PAE), an essential oil in Perilla plants, serves as a safe flavor ingredient foods, and shows effectively antifungal activity. Reactive oxygen species (ROS) accumulation Aspergillus flavus plays critical role initiating metacaspase-dependent apoptosis. However, the reason for ROS A. is not yet clear. Using transcriptome sequencing of treated with different concentrations PAE, our data showed that might have been result inhibition energy metabolism less production reducing...