A5056556606- Occupational exposure and asthma
- Indoor Air Quality and Microbial Exposure
- Contact Dermatitis and Allergies
- Mass Spectrometry Techniques and Applications
- Bacterial Identification and Susceptibility Testing
- Air Quality and Health Impacts
- Antifungal resistance and susceptibility
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Food Allergy and Anaphylaxis Research
- Fungal Infections and Studies
- Odor and Emission Control Technologies
- Allergic Rhinitis and Sensitization
- Bacteriophages and microbial interactions
- Anesthesia and Sedative Agents
- Advanced Proteomics Techniques and Applications
- Identification and Quantification in Food
- Advanced Chemical Sensor Technologies
- Pesticide Exposure and Toxicity
- Infection Control and Ventilation
- COVID-19 and healthcare impacts
- Kruppel-like factors research
- Occupational and environmental lung diseases
- MicroRNA in disease regulation
- Antimicrobial Peptides and Activities
National Institute for Occupational Safety and Health
2013-2024
Centers for Disease Control and Prevention
2008-2021
The Centers
2011-2012
Portland State University
2007
Analytical Services
2005
National Center for HIV/AIDS Viral Hepatitis STD and TB Prevention
2004
Mitchell Institute
2002
Truman State University
2001
Texas A&M University
2000-2001
Low molecular weight chemical (LMW) allergens are commonly referred to as haptens. Haptens must complex with proteins be recognized by the immune system. The majority of occupationally related haptens reactive, electrophilic chemicals, or metabolized reactive metabolites that form covalent bonds nucleophilic centers on proteins. Nonelectrophilic protein binding may occur through disulfide exchange, coordinate onto metal ions metalloproteins allergens, themselves, major histocompatibility...
New methodologies for surveillance and identification of Mycobacterium tuberculosis are required to stem the spread disease worldwide. In addition, ability discriminate mycobacteria at strain level may be important contact or source case investigations. To this end, we developing MALDI-TOF MS methods M. in culture. report, describe application MS, as well statistical analysis including linear discriminant random forest analysis, 16 medically relevant strains from four species mycobacteria,...
Universal mask wearing is recommended to help control the spread of COVID-19. Masks reduce expulsion aerosols respiratory fluids into environment (called source control) and offer some protection wearer. are often characterized using filtration efficiency, airflow resistance, manikin or human fit factors, which standard metrics used for personal protective devices. However, none these direct measurements how effectively a blocks coughed exhaled aerosols. We studied performance 15 cloth masks...
Abstract Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) was used to generate highly reproducible spectral ‘fingerprints’ for twelve Penicillium species. Prior MALDI‐TOF MS analysis, eight replicate cultures of each species were subjected three one‐minute bead‐beating cycles in an acetonitrile/trifluoroacetic acid solvent. The spectra contained abundant peaks the range m/z 5000–20 000, and allowed unambiguous discrimination between In addition, a...
Current methods for the identification of mycobacteria in culture are time-consuming, requiring as long 12 weeks positive identification. One potential approach to rapid mycobacterial is utilize proteomic profiling cultures by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In this report, we have applied MALDI-TOF MS cultured microorganisms representing six species genus Mycobacterium. We find that analysis acetonitrile/trifluoroacetic acid...
1. Occupational exposure to 4,4'-methylene diphenyl diisocyanate (MDI) is associated with occupational asthma (OA) development. Alveolar macrophage-induced recruitment of immune cells the lung microenvironment plays an important role during pathogenesis. Previous studies identified that MDI/MDI-glutathione (GSH)-exposure downregulates endogenous
Glutathione has previously been identified as a reaction target for toluene diisocyanate (TDI) in vitro and vivo, suggested to contribute toxic allergic reactions exposure. In this study, the reactivity of reduced glutathione (GSH) with TDI was further investigated using mixed phase (vapor/liquid) exposure system model vivo biophysics lower respiratory tract. HPLC/MS/MS used characterize observed products. Under conditions tested, major products between vapor GSH were S-linked bis(GSH)-TDI...
Exposure to soy antigens has been associated with asthma in community outbreaks and some workplaces. Recently, 135 flake processing workers (SPWs) a Tennessee facility were evaluated for immune reactivity soy. Allergic sensitization was common five times more prevalent than health care worker controls (HCWs) no known exposure.To characterize allergens SPWs.Sera that positive ImmunoCAP (n=27) tested IgE immunoblots. Wild-type (WT) transgenic (TG) sequenced using nanoscale Ultra-Performance...
The anti-microbial compound triclosan is incorporated into numerous consumer products and detectable in the urine of 75% general United States population. Recent epidemiological studies report positive associations with urinary levels allergic disease. Although not sensitizing, earlier previously found that repeated topical application augments response to ovalbumin (OVA) though a thymic stromal lymphopoietin (TSLP) pathway mice. In present study, early immunological effects following...
Most murine models of fungal exposure are based on the delivery uncharacterized extracts or liquid conidia suspensions using aspiration intranasal approaches. Studies that model to dry aerosols whole body inhalation have only recently been described. In this study, we aimed characterize pulmonary immune responses following repeated utilizing an acoustical generator deliver mice housed in a nose chamber. Immunocompetent female BALB/cJ were exposed derived from Aspergillus fumigatus wild-type...
DNA analysis by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is hindered two processes: alkali metal adduction and fragmentation of the intact ionized molecule. The adverse effects both processes can be reduced adding ammonium ion salts or compounds such as fructose to sample preparations. Matrix additives improve sensitivity resolution MALDI. In addition, spot-to-spot reproducibility, resolution, accuracy for oligonucleotides (≤12 mer) improved use overlayer...
Exposure to 4,4'-methylene diphenyl diisocyanate (MDI) in the occupational setting may lead development of asthma (OA), and underlying molecular mechanisms MDI-induced disease pathogenesis remain an active area research. Using a nose-only mouse inhalation model, we find that circulating microRNA (miR)-206-3p miR-381-3p are downregulated after MDI exposure; however, cellular miR-206-3p responses aerosol exposure their pathophysiological roles MDI-OA unknown. We hypothesize...
The rubber accelerator, 2-mercaptobenzothiazole (MBT), has been reported to cause allergic contact dermatitis from gloves and other products, but its chemical fate when exposed occupational oxidants the mechanism of pathogenesis are not known. It was hypothesized that thiol group is critical MBT's (its oxidation products or metabolites) covalent binding and/or haptenation nucleophilic protein residues. Oxidative transformation MBT disulfide 2,2′-dithiobis(benzothiazole) (MBTS) observed...
Diisocyanates are highly reactive chemical compounds widely used in the manufacture of polyurethanes. Although diisocyanates have been identified as causative agents allergic respiratory diseases, specific mechanism by which these diseases occur is largely unknown. To better understand species produced when isocyanates reacted with model peptides, tandem mass spectrometry was employed to unambiguously identify binding site four commercially-relevant on peptides. In each case, react...
Diisocyanates (dNCOs) are potent chemical allergens utilized in various industries. It has been proposed that skin exposure to dNCOs produces immune sensitization leading work-related asthma and allergic disease. We examined by using a dermal murine model of toluene diisocyanate (TDI) characterize the disposition TDI skin, identify predominant haptenated proteins, discern associated antigen uptake dendritic cells. Ears BALB/c mice were dosed once with (0.1% or 4% v/v acetone). draining lymph...