A5042718756- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Chromosomal and Genetic Variations
- Plant Parasitism and Resistance
- Plant Pathogenic Bacteria Studies
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Insect symbiosis and bacterial influences
- Immune Response and Inflammation
- Plant Pathogens and Fungal Diseases
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Asthma and respiratory diseases
- Tryptophan and brain disorders
Sainsbury Laboratory
2012-2021
Norwich Research Park
2012-2021
University of East Anglia
2020-2021
Essentially all plant species exhibit heritable genetic variation for resistance to a variety of diseases caused by fungi, bacteria, oomycetes or viruses. Disease losses in crop monocultures are already significant, and would be greater but applications disease-controlling agrichemicals. For sustainable intensification production, we argue that disease control should as far possible achieved using genetics rather than costly recurrent chemical sprays. The latter imply CO₂ emissions from...
Bacterial CRISPR systems have been widely adopted to create operator-specified site-specific nucleases. Such nuclease action commonly results in loss-of-function alleles, facilitating functional analysis of genes and gene families We conducted a systematic comparison components T-DNA architectures for CRISPR-mediated editing Arabidopsis, testing multiple promoters, terminators, sgRNA backbones Cas9 alleles. identified architecture that usually stable (i.e. homozygous) mutations the first...
Plant NLR (Nucleotide-binding domain and Leucine-rich Repeat) immune receptor proteins are encoded by Resistance (R) genes confer specific resistance to pathogen races that carry the corresponding recognized effectors. Some function in pairs, forming complexes for perception of We show here Arabidopsis RPS4 RRS1 both required make an authentic complex. Over-expression tobacco or results constitutive defense activation; this phenotype is suppressed presence RRS1. protein co-immunoprecipitates...
The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 single guide RNAs (sgRNA) to target the domain, recovered several dominant dwarf mutation that carries deletion of one amino acid in domain. This is first report allele. allele retains partial responsiveness exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at seedling stage, but adult heterozygotes are as dwarfed homozygotes.
Plant nucleotide-binding domain, leucine-rich repeat receptor (NLR) proteins play important roles in recognition of pathogen-derived effectors. However, the mechanism by which plant NLRs activate immunity is still largely unknown. The paired Arabidopsis RRS1-R and RPS4, that confer bacterial effectors AvrRps4 PopP2, are well studied, but how RRS1/RPS4 complex activates early immediate downstream responses upon effector detection poorly understood. To study without influence cell surface...
The interaction between tomato and the leaf mold pathogen Cladosporium fulvum is controlled in a gene-for-gene manner by plant Cf genes that encode membrane-anchored extracytoplasmic leucine-rich repeat (LRR) glycoproteins, which confer recognition of their cognate fungal avirulence (Avr) proteins. Cf-9 Cf-4 are two such proteins 91% identical yet recognize sequence-unrelated determinants Avr9 Avr4, respectively. As shown previously, specificity determined three putative solvent-exposed...
Abstract Late blight caused by Phytophthora infestans greatly constrains potato production. Many Resistance (R) genes were cloned from wild Solanum species and/or introduced into cultivars breeding. However, individual R have been overcome P. evolution; durable resistance remains elusive. We positionally a new gene, Rpi-amr1 , americanum that encodes an NRC helper-dependent CC-NLR protein. confers in to all 19 isolates tested. Using association genomics and long-read RenSeq, we defined eight...
ABSTRACT Bacterial CRISPR systems have been widely adopted to create operator-specified site-specific nucleases. Such nuclease action commonly results in loss-of-function alleles, facilitating functional analysis of genes and gene families We conducted a systematic comparison components T-DNA architectures for CRISPR-mediated editing Arabidopsis, testing multiple promoters, terminators, sgRNA backbones Cas9 alleles. identified architecture that usually stable ( i.e. homozygous) mutations the...
Abstract Plant nucleotide-binding domain, leucine-rich repeat receptor (NLR) proteins play important roles in recognition of pathogen-derived effectors. However, the mechanism by which plant NLRs activate immunity is still largely unknown. The paired Arabidopsis RRS1-R and RPS4, that confer bacterial effectors AvrRps4 PopP2, are well studied, but how RRS1/RPS4 complex activates early immediate downstream responses upon effector detection poorly understood. To study without influence...