A5055965037- Mycotoxins in Agriculture and Food
- Fungal and yeast genetics research
- Plant Pathogens and Fungal Diseases
- Essential Oils and Antimicrobial Activity
- Fungal Biology and Applications
- Antifungal resistance and susceptibility
- Plant-Microbe Interactions and Immunity
- Piperaceae Chemical and Biological Studies
- Fungal Infections and Studies
- Microbial Natural Products and Biosynthesis
- Plant Disease Resistance and Genetics
- Algal biology and biofuel production
- Advanced Proteomics Techniques and Applications
- Coconut Research and Applications
- Aluminum toxicity and tolerance in plants and animals
- Heavy Metal Exposure and Toxicity
- Nematode management and characterization studies
- Genomics, phytochemicals, and oxidative stress
- Signaling Pathways in Disease
- Microbial Metabolism and Applications
- Marine Toxins and Detection Methods
- Selenium in Biological Systems
- Olfactory and Sensory Function Studies
- Echinoderm biology and ecology
- Traditional and Medicinal Uses of Annonaceae
Jiangsu Normal University
2019-2025
University of Wisconsin–Madison
2018-2025
Fujian Agriculture and Forestry University
2014-2020
Abstract DNA methylation is essential for epigenetic regulation of gene transcription and development in many animals, plants fungi. We investigated whether plays a role the secondary metabolism Aspergillus flavus , identified DmtA methyltransferase from A. produced dmtA knock-out mutant by replacing coding sequence with pyrG selectable marker. The null lines white fluffy mycelium liquid medium displayed slightly flavescent conidial pigmentation compared normal yellow wild-type strain when...
The problem of food spoilage due to Aspergillus flavus (A. flavus) needs be resolved. In this study, we found that the minimum inhibitory concentration cinnamaldehyde (CA) inhibited A. was 0.065 mg/ml and corn can prevented from spoiling at a 0.13 mg/cm3. addition inhibiting spore germination, mycelial growth, biomass production, CA also reduce ergosterol synthesis cause cytomembrane damage. Our intention elucidate antifungal mechanism CA. Flow cytometry, fluorescence microscopy, western...
Summary Aspergillus flavus is an opportunistic fungal pathogen that colonizes agriculture crops with aflatoxin contamination. We found Perillaldehyde (PAE) effectively inhibited A . viability and production by inducing excess reactive oxygen species (ROS). Transcriptome analysis indicated the Gα protein FadA was significantly induced PAE. Functional characterization of showed it important for asexual development biosynthesis regulation cAMP‐PKA signalling. The Δ fadA mutant more sensitive to...
Histone acetyltransferases (HATs) help regulate fungal development and the production of secondary metabolites. In this study, we determined that HAT AflGcnE influenced morphogenesis aflatoxin biosynthesis in Aspergillus flavus. We observed localized to nucleus cytoplasm during conidial germination stages, while it was located mainly hyphal stage. Deletion AflgcnE inhibited growth A. flavus decreased hydrophobicity cell surface. The ΔAflgcnE mutant exhibited a lack asexual sporulation unable...
This study investigated the impact of dietary fiber from sweet potato residue (SPDF) on diversity gut microbiota.
In Aspergillus nidulans, the nitrogen metabolite repression regulator NmrA plays a major role in regulating activity of GATA transcription factor AreA during metabolism. However, function nmrA flavus has not been previously studied. Here, we report identification and functional analysis A. flavus. Our work showed that amino acid sequences are highly conserved among species protein contains canonical Rossmann fold motif. Deletion slowed growth but significantly increased conidiation sclerotia...
This study focused on AflSkn7, which is a stress response regulator in the aflatoxin-producing Aspergillus flavus. The ΔAflSkn7 mutants exhibited partially defective conidial formation and complete inability to generate sclerotia, indicating AflSkn7 affects A. flavus asexual sexual development. tolerated osmotic but were susceptible effects of cell wall stress. Additionally, especially sensitive oxidative These observations confirmed that influences responses rather than responses. was...
Perillaldehyde (PAE), an essential oil in Perilla plants, serves as a safe flavor ingredient foods, and shows effectively antifungal activity. Reactive oxygen species (ROS) accumulation Aspergillus flavus plays critical role initiating metacaspase-dependent apoptosis. However, the reason for ROS A. is not yet clear. Using transcriptome sequencing of treated with different concentrations PAE, our data showed that might have been result inhibition energy metabolism less production reducing...
Aspergillus flavus is an opportunistic pathogen of crops, including peanuts and maize, the second leading cause aspergillosis in immunocompromised patients. A. also a major producer mycotoxin, aflatoxin, potent carcinogen, which results significant crop losses annually. The isolate NRRL 3357 was originally isolated from peanut has been used as model organism for understanding regulation production secondary metabolites, such aflatoxin. A draft genome previously constructed, enabling...
The ubiquitous fungus Aspergillus flavus is notorious for contaminating many important crops and food-stuffs with the carcinogenic mycotoxin, aflatoxin. This also second most frequent pathogen after A. fumigatus infecting immunosuppressed patients. In human fungal pathogens including fumigatus, ability to defend from toxic levels of copper (Cu) essential in pathogenesis. Cu-fist DNA binding protein, AceA, Cu ATPase transporter, CrpA, play critical roles defense. Here, we show that tolerates...
Aspergillus flavus is a renowned plant, animal and human pathogen. areA global nitrogen regulatory gene of the GATA transcription factor family, shown to be major regulator. In this study, we identified in A. studied its function. The AreA protein contained signatory zinc finger domain, which extremely conserved across fungal species. Gene deletion (ΔareA) over-expression (OE::areA) strains were constructed by homologous recombination elucidate role flavus. ΔareA strain was unable...
In this study, the aim was to investigate inhibitory effect of 6,6'-bieckol on migration and epithelial-mesenchymal transition (EMT) non-small cell lung cancer (NSCLC) cells, explore its potential molecular mechanisms.Cell measured using a CCK8, wound healing, transwell assay. Apoptosis determined an Annexin V/propidium iodide staining. Western blotting immunofluorescence were used examine expression level apoptosis-related proteins EMT marker proteins.The results showed that inhibited...
Aspergillus flavus, a ubiquitous filamentous fungus found in soil, plants and other substrates has been reported not only as pathogen for plants, but also carcinogen producing human. Peptidyl-Prolyl Isomerase (PPIases) plays an important role cell process such protein secretion cycle control RNA processing. However, the function of PPIase yet identified A. flavus. In this study, PPIases gene from flavus named ppci1 was cloned into expression vector expressed prokaryotic system. Activity...
Autophagy, a conserved cellular recycling process, plays crucial role in maintaining homeostasis under stress conditions. It also regulates the development and virulence of numerous filamentous fungi. In this study, we investigated specific function ATG8, reliable autophagic marker, opportunistic pathogen