A5103577931- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Aluminum toxicity and tolerance in plants and animals
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant and Biological Electrophysiology Studies
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Magnetic and Electromagnetic Effects
- GABA and Rice Research
- Bone Tissue Engineering Materials
- Plant tissue culture and regeneration
- Plant Micronutrient Interactions and Effects
- Mechanisms of cancer metastasis
- Plant Gene Expression Analysis
- Spine and Intervertebral Disc Pathology
- Plant Pathogenic Bacteria Studies
- Heterotopic Ossification and Related Conditions
- Ion channel regulation and function
- Bone fractures and treatments
- Cancer Mechanisms and Therapy
- Ion Transport and Channel Regulation
- Mitochondrial Function and Pathology
- Plant biochemistry and biosynthesis
- Bone Metabolism and Diseases
Osaka University
2023-2025
Hagoromo International University
2021-2022
Nagoya University
2005-2018
Okayama University
2010-2018
Nagoya Keizai University
2016
Gifu Women's University
2014
Okayama Prefectural Kurashiki Chuo High School
2010
Graduate School USA
2006-2009
Institute of Biosciences and Bioresources
2009
Friedrich-Alexander-Universität Erlangen-Nürnberg
2007-2008
Plants can sense and respond to mechanical stimuli, like animals. An early mechanism of mechanosensing response is speculated be governed by as-yet-unidentified sensory complexes containing a Ca 2+ -permeable, stretch-activated (SA) channel. However, the components or regulators such are poorly understood at molecular level in plants. Here, we report identification plasma membrane protein (designated Mca1) that correlates influx with Arabidopsis thaliana . MCA1 cDNA was cloned functional...
Plant stomata limit both carbon dioxide uptake and water loss; hence, stomatal aperture is carefully set as the environment fluctuates. Aperture area known to be regulated in part by ion transport, but few of transporters have been characterized. Here we report that AtALMT12 (At4g17970), a homolog aluminum-activated malate transporter (ALMT) wheat, expressed guard cells Arabidopsis thaliana. Loss-of-function mutations impair closure induced ABA, calcium darkness, do not abolish either...
Background and Aims Regulation of water channel aquaporins (AQPs) provides another mechanism by which abscisic acid (ABA) may influence flow through plants. To the best our knowledge, no studies have addressed changes in ABA levels, abundance AQPs root cell hydraulic conductivity (LpCell) same tissues. Thus, we followed mechanisms affects hydraulics an ABA-deficient barley mutant Az34 its parental line 'Steptoe'. We compared cells to determine spatial correlations between AQP local...
The gene encoding voltage-gated channel with high affinity for Ca2+ permeation has not been cloned from plants. In the present study, we isolated a full-length cDNA putative (AtTPC1) Arabidopsis. AtTPC1 two conserved homologous domains, both of which contain six transmembrane segments (S1–S6) and pore loop (P) between S5 S6 in each domain, highest homology TPC1 recently rat. overall structure is similar to half general α-subunits voltage-activated channels animals. rescued uptake activity...
Plants respond to a large variety of environmental signals, including changes in the gravity vector (gravistimulation). In Arabidopsis (Arabidopsis thaliana) seedlings, gravistimulation is known increase cytoplasmic free calcium concentration ([Ca2+]c). However, organs responsible for [Ca2+]c and underlying cellular/molecular mechanisms remain be solved. this study, using seedlings expressing apoaequorin, Ca2+-sensitive luminescent protein combination with an ultrasensitive photon counting...
Higher plants sense and respond to osmotic mechanical stresses such as turgor, touch, flexure gravity. Mechanosensitive (MS) channels, directly activated by tension in the cell membrane cytoskeleton, are supposed be involved volume regulation under hypotonic conditions sensing of these based on electrophysiological pharmacological studies. However, limited progress has been achieved molecular identification plant MS channels. Here, we show that MCA1 (mid1-complementing activity 1; a putative...
Al3+-resistant cultivars of wheat (Triticum aestivum L.) release malate through the Al3+-activated anion transport protein Triticum aluminum-activated transporter 1 (TaALMT1). Expression TaALMT1 in Xenopus oocytes and tobacco suspension cells enhances basal activity (inward outward currents present absence external Al3+), generates same (reflecting Al3+-dependent function) as observed cells. We investigated amino acid residues involved this by generating a series mutations to protein....
A variety of plant species emit volatile compounds in response to mechanical stresses such as herbivore attack. Although these promote gene expression leading anti-herbivore responses, the underlying transduction mechanisms are largely unknown. While indirect evidence suggests that cytoplasmic free Ca(2+) concentration ([Ca(2+)](c)) plays a crucial role volatile-sensing plants, roles have not been directly demonstrated. In present study, we used Arabidopsis leaves expressing apoaequorin,...
Aims Nonunion occurs when a fracture fails to heal permanently, often necessitating surgical intervention stimulate the bone healing response. Current animal models of long-bone nonunion do not adequately replicate human pathological conditions. This study was intended as preliminary investigation novel rat model using two-stage intervention, and evaluate efficacy selective prostaglandin E2 receptor 4 agonist (AKDS001) therapeutic agent compared with existing treatments. Methods Initially,...
Salicylic acid (SA) is a natural signaling molecule involved in plant defense response against pathogen infection. This article covers the recent key works contributing to our understanding of SA and biosynthesis leading controlled level plants.
Methylglyoxal (MG) is a reactive aldehyde derived by glycolysis. In Arabidopsis, MG inhibited light-induced stomatal opening in dose-dependent manner. It significantly both inward-rectifying potassium (K(in)) channels guard-cell protoplasts and an Arabidopsis K(in) channel, KAT1, heterologously expressed Xenopus oocytes. Thus it appears that inhibition of involves K(+) influx into guard cells.
Abstract Gravity is a critical environmental factor affecting the morphology and functions of organisms on Earth. Plants sense changes in gravity vector (gravistimulation) regulate their growth direction accordingly. In Arabidopsis (Arabidopsis thaliana) seedlings, gravistimulation, achieved by rotating specimens under ambient 1g Earth, known to induce biphasic (transient sustained) increase cytoplasmic calcium concentration ([Ca2+]c). However, [Ca2+]c genuinely caused gravistimulation has...
Using Ca(2+)-dependent photoprotein aequorin-transformed Arabidopsis thaliana, sugar-induced increase in cytosolic free Ca(2+ )concentration ([Ca(2+)](cyt))( )was investigated by luminescence imaging technique. When 0.1 M sucrose was fed to roots of autotrophically grown intact whole plants whose had been incubated overnight with coelenterazine reconstitute aequorin systemically, strong and transient (within 20 s) observed the roots; that followed weak moving from lower leaves upper leaves....
Aluminium is well known to inhibit plant elongation, but the role in this inhibition played by water relations remains unclear. To investigate this, tobacco (Nicotiana tabacum L.) suspension-cultured cells (line SL) was used, treating them with aluminium (50 microM) a medium containing calcium, sucrose, and MES (pH 5.0). Over an 18 h treatment period, inhibited increase fresh weight almost completely decreased cellular osmolality internal soluble sugar content substantially; however, did not...
Wheat and Arabidopsis plants respond to aluminum (Al) ions by releasing malate from their root apices via Al-activated transporter. Malate anions bind with the toxic Al contribute tolerance of these species. The genes encoding transporters in wheat Arabidopsis, TaALMT1 AtALMT1, respectively, were expressed Xenopus laevis oocytes characterized electrophysiologically using two-electrode voltage clamp system. currents generated efflux detected for but not AtALMT1. Chimeric proteins swapping N-...