A5048971230- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Plant Molecular Biology Research
- Plant Parasitism and Resistance
- Nematode management and characterization studies
- Sesquiterpenes and Asteraceae Studies
- Medicinal plant effects and applications
- Plant tissue culture and regeneration
- Toxin Mechanisms and Immunotoxins
- Plant Reproductive Biology
- Heat shock proteins research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Silymarin and Mushroom Poisoning
- Polysaccharides and Plant Cell Walls
- Botanical Research and Chemistry
- Plant and Fungal Species Descriptions
- GABA and Rice Research
- Plant Disease Management Techniques
- Photosynthetic Processes and Mechanisms
- Plant and Biological Electrophysiology Studies
- Plant nutrient uptake and metabolism
- Plant Diversity and Evolution
- Transgenic Plants and Applications
ProQuest (United States)
2024
RIKEN Center for Sustainable Resource Science
2015-2024
National Institute of Diabetes and Digestive and Kidney Diseases
2024
Sainsbury Laboratory
2011-2021
University of East Anglia
2019-2021
Norwich Research Park
2010-2020
Tokyo University of Science
2004-2011
Noda Institute for Scientific Research
2008
Science Museum
2007
The University of Tokyo
2005
Plants rely heavily on receptor-like kinases (RLKs) for perception and integration of external internal stimuli. The Arabidopsis regulatory leucine-rich repeat RLK (LRR-RLK) BAK1 is involved in steroid hormone responses, innate immunity, cell death control. Here, we describe the differential regulation three different BAK1-dependent signaling pathways by a novel allele BAK1, bak1-5. Innate immune mediated RKs FLS2 EFR severely compromised bak1-5 mutant plants. However, mutants are not...
Plant respiratory burst oxidase homolog (rboh) proteins, which are homologous to the mammalian 91-kDa glycoprotein subunit of phagocyte (gp91(phox)) or NADPH 2 (NOX2), have been implicated in production reactive oxygen species (ROS) both stress responses and during development. Unlike gp91(phox)/NOX2 protein, plant rboh proteins hydrophilic N-terminal regions containing two EF-hand motifs, suggesting that their activation is dependent on Ca(2+). However, significance Ca(2+) binding motifs...
Innate immunity relies on the perception of pathogen-associated molecular patterns (PAMPs) by pattern-recognition receptors (PRRs) located host cell's surface. Many plant PRRs are kinases. Here, we report that Arabidopsis receptor kinase EF-TU RECEPTOR (EFR), which perceives elf18 peptide derived from bacterial elongation factor Tu, is activated upon ligand binding phosphorylation its tyrosine residues. Phosphorylation a single residue, Y836, required for activation EFR and downstream to...
Abstract Cell-surface receptors play pivotal roles in many biological processes, including immunity, development, and reproduction, across diverse organisms. How cell-surface evolve to become specialised different processes remains elusive. To shed light on the immune-specificity of receptors, we analyzed more than 200,000 genes encoding from 350 genomes traced evolutionary origin immune-specific leucine-rich repeat receptor-like proteins (LRR-RLPs) plants. Surprisingly, discovered that...
Abstract SGT1 (for suppressor of G2 allele skp1) and RAR1 required for Mla12 resistance) are highly conserved eukaryotic proteins that interact with the molecular chaperone HSP90 heat shock protein90). In plants, SGT1, RAR1, essential disease resistance triggered by a number (R) proteins. Here, we present structural functional characterization plant Random mutagenesis Arabidopsis thaliana SGT1b revealed its CS CHORD-SGT1) SGS specific) domains resistance. NMR-based interaction surface...
Plant NADPH oxidases, also known as respiratory burst oxidase homologues (RBOHs), produce reactive oxygen species (ROS) that perform a wide range of functions. RbohD and RbohF, two the 10 Rboh genes present in Arabidopsis, are pleiotropic mediate diverse physiological processes including response to pathogens. We hypothesized spatio-temporal control RbohF gene expression might be critical determining their multiplicity Transgenic Arabidopsis plants with promoter fusions β-glucuronidase...
Plasma membrane-localized pattern recognition receptors (PRRs) such as FLAGELLIN SENSING2 (FLS2), EF-TU RECEPTOR (EFR), and CHITIN ELICITOR KINASE1 (CERK1) recognize microbe-associated molecular patterns (MAMPs) to activate pattern-triggered immunity (PTI). A reverse genetics approach on genes responsive the priming agent β-aminobutyric acid (BABA) revealed IMPAIRED OOMYCETE SUSCEPTIBILITY1 (IOS1) a critical PTI player. Arabidopsis thaliana ios1 mutants were hypersusceptible Pseudomonas...
Summary Plant immunity consists of two arms: pathogen‐associated molecular pattern (PAMP)‐triggered (PTI), induced by surface‐localized receptors, and effector‐triggered (ETI), intracellular receptors. Despite the little structural similarity, both receptor types activate similar responses with different dynamics. To better understand phosphorylation events during ETI, we employed a phosphoproteomic screen using an inducible expression system bacterial effector avrRpt2 in Arabidopsis...
Plants use receptor kinases (RKs) and receptor-like proteins (RLPs) as pattern recognition receptors (PRRs) to sense pathogen-associated molecular patterns (PAMPs) that are typical of whole classes microbes. After ligand perception, many leucine-rich repeat (LRR)-containing PRRs interact with the LRR-RK BRI1-ASSOCIATED KINASE 1 (BAK1). BAK1 is thus expected unknown PRRs. Here, we used bait identify a previously LRR-RLP required for csp22 peptide derived from bacterial cold shock protein. We...
Abstract Enzyme biosensors are useful tools that can monitor rapid changes in metabolite levels real-time. However, current approaches largely constrained to metabolites within a limited chemical space. With the rising development of artificial metalloenzymes (ArM), unique opportunity exists design from ground-up for difficult detect using technologies. Here we present and ArM ethylene probe ( AEP ), where an albumin scaffold is used solubilize protect quenched ruthenium catalyst. In...
The plasma membrane (PM) is composed of heterogeneous subdomains, characterized by differences in protein and lipid composition. PM receptors can be dynamically sorted into domains to underpin signaling response extracellular stimuli. In plants, the plasmodesmal a discrete microdomain that hosts specific responses. We exploited independence this domain investigate how independently integrate signal triggers responses across cell. Focusing on chitin signaling, we found require LysM receptor...
Hsp90-mediated function of NLR receptors in plant and animal innate immunity depends on the cochaperone Sgt1 and, at least plants, a cysteine- histidine-rich domains (CHORD)-containing protein Rar1. Functionally, CHORD are associated with CS domains, either within same protein, as mammalian melusin Chp1, or separate but interacting proteins, Rar1 Sgt1. Both independently capable molecular chaperone Hsp90 can coexist complexes Hsp90. We have now determined structure an Hsp90-CS-CHORD ternary...
Plant resistance inducers (PRIs) are compounds that protect plants from diseases by activating immunity responses. Exogenous treatment with glutamate (Glu), an important amino acid for all living organisms, induces against fungal pathogens in rice and tomato. To understand the molecular mechanisms of Glu-induced immunity, we used Arabidopsis model system. We found exogenous Glu Arabidopsis. Consistent this, transcriptome analyses seedlings showed significantly expression wound-, defense-,...
Ion fluxes and the production of reactive oxygen species (ROS) are early events that follow elicitor treatment or microbial infection. However, molecular mechanisms for these responses as well their relationship have been controversial still largely unknown. We here simultaneously monitored temporal sequence initial at plasma membrane in suspension-cultured tobacco cells (cell line BY-2) response to a purified proteinaceous elicitor, cryptogein, which induced hypersensitive cell death. The...
Plant-parasitic nematodes (PPNs) cause major agricultural losses worldwide, yet the molecular basis of plant immunity against these pathogens remains poorly understood. To investigate how plants recognize PPNs, we aimed to identify microbe-associated patterns (MAMPs) from and corresponding immune components. Due limited availability material obligate used Caenorhabditis elegans , a free-living nematode, as MAMP source. C. extracts activated MAMP-triggered responses in Arabidopsis Col-0....