A5003493684- Plant Parasitism and Resistance
- Insect-Plant Interactions and Control
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Toxin Mechanisms and Immunotoxins
- Plant Stress Responses and Tolerance
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Cassava research and cyanide
- Photosynthetic Processes and Mechanisms
- Heat shock proteins research
- Plant nutrient uptake and metabolism
- Nitrogen and Sulfur Effects on Brassica
- Light effects on plants
- Plant Gene Expression Analysis
- Enzyme Structure and Function
- Trace Elements in Health
- Transgenic Plants and Applications
- Plant biochemistry and biosynthesis
- Plant Genetic and Mutation Studies
- Isotope Analysis in Ecology
- Insect and Pesticide Research
- Vector-borne infectious diseases
- Protein Structure and Dynamics
Universidad Politécnica de Madrid
2019-2024
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2020-2024
Centre for Plant Biotechnology and Genomics
2020-2024
Consejo Superior de Investigaciones Científicas
2004-2023
Centro Nacional de Biotecnología
2014-2022
Hudson Institute
2018-2020
John Wiley & Sons (United Kingdom)
2018-2020
John Innes Centre
2007-2010
Sainsbury Laboratory
2007-2010
University of York
2007
Jasmonates (JA) are important regulators of plant defense responses that activate expression many wound-induced genes including the tomato proteinase inhibitor II ( pin2 ) and leucine aminopeptidase LAP genes. Elements required for JA induction gene all present in -317 to -78 proximal promoter region. Using yeast one-hybrid screening, we have identified bHLH-leu zipper JAMYC2 JAMYC10 proteins, specifically recognizing a T/G-box AACGTG motif this fragment. Mutation G-box element decreases...
Pathogenicity of Pseudomonas syringae is dependent on a type III secretion system, which secretes suite virulence effector proteins into the host cytoplasm, and production number toxins such as coronatine (COR), mimic plant hormone jasmonate-isoleuce (JA-Ile). Inside cell, effectors target molecules to subvert cell physiology disrupt defenses. However, despite fact that elucidating action essential understanding bacterial pathogenesis, molecular function targets vast majority remain largely...
Abstract SGT1 (for suppressor of G2 allele skp1) and RAR1 required for Mla12 resistance) are highly conserved eukaryotic proteins that interact with the molecular chaperone HSP90 heat shock protein90). In plants, SGT1, RAR1, essential disease resistance triggered by a number (R) proteins. Here, we present structural functional characterization plant Random mutagenesis Arabidopsis thaliana SGT1b revealed its CS CHORD-SGT1) SGS specific) domains resistance. NMR-based interaction surface...
The potato (Solanum tuberosum) nucleotide binding-leucine-rich repeat immune receptor Rx confers resistance to Potato virus X (PVX) and requires Ran GTPase-activating protein 2 (RanGAP2) for effective signaling. Although does not contain a discernible nuclear localization signal, the localizes both cytoplasm nucleus in Nicotiana benthamiana. Transient coexpression of cytoplasmically localized RanGAP2 sequesters cytoplasm. This relocation appeared be mediated by physical interaction between...
Coronatine (COR) facilitates entry of bacteria into the plant apoplast by stimulating stomata opening. COR-induced signaling events at remain unclear. We found that COR and jasmonate isoleucine (JA-Ile) co-receptor JAZ2 is constitutively expressed in guard cells modulates stomatal dynamics during bacterial invasion analyzed tissue expression patterns AtJAZ genes measured opening pathogen resistance loss- gain-of-function mutants. Arabidopsis jaz2 mutants are partially impaired...
The plant hormone jasmonate (JA) plays an important role in regulating growth, development, and immunity. Activation of the JA-signaling pathway is based on hormone-triggered ubiquitination removal transcriptional repressors (JASMONATE-ZIM DOMAIN [JAZ] proteins) by SCF receptor complex (SCF(COI1)/JAZ). This allows rapid activation transcription factors (TFs) triggering a multitude downstream responses. Identification TFs bound JAZ proteins essential to better understand how modulates...
Jasmonate (JA) signaling in plants is mediated by the JASMONATE ZIM-DOMAIN (JAZ) proteins that repress activity of several transcription factors regulating JA-inducible gene expression. The hormone JA-isoleucine triggers interaction JAZ repressor with F-box protein CORONATINE INSENSITIVE1 (COI1), part an S-phase kinase-associated protein1/Cullin1/F-box COI1 (SCFCOI1) E3 ubiquitin ligase complex, and their degradation 26S proteasome. In Arabidopsis (Arabidopsis thaliana), family consists 13...
Seed germination is a complex trait determined by the interaction of hormonal, metabolic, genetic, and environmental components. Variability this in crops has big impact on seedling establishment yield field. Classical studies have focused mainly analyses one level regulation cascade events leading to seed germination. We carried out an integrative extensive approach deepen our understanding Brassica napus generating transcriptomic, hormonal data at different stages upon imbibition. Deep...
The tetratricopeptide repeat (TPR) domain mediates inter-protein associations in a number of systems. is also thought to mediate oligomerization some proteins, but this has remained controversial, with conflicting data appearing the literature. By way investigating such TPR-mediated self-associations we used variety biophysical techniques characterize purified recombinant Sgt1, TPR-containing protein found all eukaryotes that involved broad range biological processes, including kinetochore...
Disease resistance in plants requires the activation of defense signaling pathways to prevent spread infection. The protein Required for Mla12 Resistance (RAR1) is a component such pathways, which contains cysteine- and histidine-rich domains (CHORDs) that bind zinc. CHORDs are 60 amino acid domains, usually arranged tandem, found almost all eukaryotes, where they involved processes ranging from pressure sensing heart maintenance diploidy fungi, exhibit distinct protein−protein interaction...
Elevated growth temperatures are negatively affecting crop productivity by increasing yield losses. The modulation of root traits associated with improved response to rising is a promising approach generate new varieties better suited face the environmental constraints caused climate change. In this study, we identified several Brassica napus altered in warm ambient temperatures. Different combinations changes specific result an extended and deeper system. This overall expansion facilitates...
Understanding the complex interactions between plants and herbivores is essential for improving crop resistance. Aiming to expand role of cyanogenesis in plant defence, we investigated response cyanogenic Phaseolus lunatus (lima bean) non-cyanogenic vulgaris (common Tetranychus urticae (spider mite) infestation. Despite mite infesting both legumes, leaf damage infringed by this feeder was reduced lima bean. Comparative transcriptome analyses revealed that species exhibited substantial...