A5025914718- Plant-Microbe Interactions and Immunity
- Nematode management and characterization studies
- Plant Virus Research Studies
- Legume Nitrogen Fixing Symbiosis
- Transgenic Plants and Applications
- Plant Pathogenic Bacteria Studies
- Monoclonal and Polyclonal Antibodies Research
- Entomopathogenic Microorganisms in Pest Control
- Plant Parasitism and Resistance
- Toxin Mechanisms and Immunotoxins
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Immune Response and Inflammation
- Insect Resistance and Genetics
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Immune Cell Function and Interaction
- Insects and Parasite Interactions
- Potato Plant Research
- Genomics and Chromatin Dynamics
- NF-κB Signaling Pathways
- Spectroscopy Techniques in Biomedical and Chemical Research
- Optical Imaging and Spectroscopy Techniques
- Polysaccharides and Plant Cell Walls
Wageningen University & Research
2009-2022
Graduate School Experimental Plant Sciences
2013-2017
Centre for BioSystems Genomics
2012
Massachusetts General Hospital
2004
Harvard University
2004
Abstract The Rx1 protein, as many resistance proteins of the nucleotide binding–leucine-rich repeat (NB-LRR) class, is predicted to be cytoplasmic because it lacks discernable nuclear targeting signals. Here, we demonstrate that Rx1, which confers extreme Potato virus X, located both in nucleus and cytoplasm. Manipulating nucleocytoplasmic distribution or its elicitor revealed activated cytoplasm cannot nucleus. coiled coil (CC) domain was found required for accumulation nucleus, whereas LRR...
The potato (Solanum tuberosum) nucleotide binding-leucine-rich repeat immune receptor Rx confers resistance to Potato virus X (PVX) and requires Ran GTPase-activating protein 2 (RanGAP2) for effective signaling. Although does not contain a discernible nuclear localization signal, the localizes both cytoplasm nucleus in Nicotiana benthamiana. Transient coexpression of cytoplasmically localized RanGAP2 sequesters cytoplasm. This relocation appeared be mediated by physical interaction between...
Genes encoding plant nucleotide-binding leucine-rich repeat (NB-LRR) proteins confer dominant resistance to diverse pathogens. The wild-type potato NB-LRR protein Rx confers against a single strain of virus X (PVX), whereas LRR mutants protect both second PVX and the distantly related poplar mosaic (PopMV). In one there was cost broad-spectrum because response PopMV transformed from mild disease on plants carrying trailing necrosis that killed plant. To explore use secondary mutagenesis...
Three new genera, six species, three combinations, epitypes, and 25 interesting host /or geographical records are introduced in this study. New genera: Neoleptodontidium (based on aquaticum ), Nothoramularia ragnhildianicola ). species: Acremonium (from cooling pad water, USA, Cladophialophora laricicola (on dead wood of Larix sp., Netherlands), Cyphellophora neerlandica lichen brick wall, Geonectria muralis moss growing a Harposporium illinoisense rockwool, USA), hydroponic USA)....
Abstract The potato cyst nematode Globodera rostochiensis invades roots of host plants where it transforms cells near the vascular cylinder into a permanent feeding site. cell modifications are most likely induced by complex mixture proteins in stylet secretions nematodes. Resistance to nematodes conferred nucleotide-binding-leucine-rich repeat (NB-LRR) usually results programmed death and around site, is triggered recognition effectors secretions. However, actual role these activation...
Many plant and animal immune receptors have a modular nucleotide-binding-leucine-rich repeat (NB-LRR) architecture in which nucleotide-binding switch domain, NB-ARC, is tethered to LRR sensor domain. The cooperation between the domains, regulates activation of these proteins, poorly understood. Here, we report structural determinants governing interaction NB-ARC highly homologous Gpa2 Rx1, recognize potato cyst nematode Globodera pallida Potato virus X, respectively. Systematic shuffling...
The rust fungus Uromyces plumbarius Peck was reported in Europe for the first time on Oenothera lindheimeri (Engelm. & A. Gray) W.L. Wagner Hoch (Lindheimer's beeblossom), a highly popular ornamental plant widely cultivated since 2000s. found 11 locations across France and two Netherlands. identification based an analysis of morphological characteristics ITS rDNA sequences. pathogenicity confirmed through reinoculation using fresh urediniospores. This represents record U. outside its...
Abstract The potato immune receptor Gpa2 confers host-specific resistance to the cyst nematode Globodera pallida. When transiently expressed in Nicotiana benthamiana it triggers cell death upon recognition of matching effector GpRBP-1. Effector-triggered immunity by depends on host factor RanGAP2, which is known regulate nucleocytoplasmic distribution and functioning highly homologous Rx1. However, subcellular localisation role RanGAP2 determining yet unknown. Moreover, cellular mechanisms...
Plant nucleotide-binding leucine-rich repeat (NLR) proteins enable cells to respond pathogen attack. Several NLRs act in the nucleus; however, conserved nuclear targets that support their role immunity are unknown. Previously, we noted a structural homology between domain of and DNA replication origin-binding Cdc6/Orc1 proteins. Here show NB-ARC (nucleotide-binding, Apaf-1, R-proteins, CED-4) Rx1 NLR potato binds nucleic acids. induces ATP-dependent bending melting vitro, dependent upon...
Plant resistance (R) proteins mediate race-specific immunity and initiate host defenses that are often accompanied by a localized cell-death response. Most R belong to the nucleotide binding-leucine-rich repeat (NB-LRR) protein family, as they carry central NB-ARC domain fused an LRR domain. The coiled-coil (CC) at N terminus of some solanaceous NB-LRR is extended with solanaceae (SD). Tomato Mi-1.2, which confers against nematodes, white flies, psyllids, aphids, encodes typical SD-CNL...
Plants have evolved a limited repertoire of NB-LRR disease resistance (R) genes to protect themselves against myriad pathogens. This limitation is thought be counterbalanced by the rapid evolution proteins, as only few sequence changes been shown sufficient alter specificities toward novel strains pathogen. However, little known about flexibility R switch between phylogenetically unrelated To investigate this, we created domain swaps close homologs Gpa2 and Rx1, which confer in potato...
In vivo imaging of endogenously expressed mammalian proteases has been useful for the detection cancer and preneoplastic lesions, staging inflammatory autoimmune diseases, testing efficacy novel protease inhibitors. Here we report on synthesis a probe that is specific HIV-1 (PR). The was designed to be biocompatible, i.v. injectable, detectable by fluorescence imaging. Human Gli36 glioblastoma cells infected with an human simplex virus amplicon vector expressing HIV-1PR showed activation,...
The intracellular immune receptor Rx1 of potato (Solanum tuberosum), which confers effector-triggered immunity to Potato virus X, consists a central nucleotide-binding domain (NB-ARC) flanked by carboxyl-terminal leucine-rich repeat (LRR) and an amino-terminal coiled-coil (CC) domain. activity is strictly regulated interdomain interactions between the NB-ARC LRR, but contribution CC in regulating or signaling not fully understood. Therefore, we used structure-informed approach investigate...
Plant-parasitic nematodes secrete effectors that manipulate plant cell morphology and physiology to achieve host invasion establish permanent feeding sites. Effectors from the highly expanded SPRYSEC (SPRY domain with a signal peptide for secretion) family in potato cyst have been implicated activation suppression of immunity, but mechanisms underlying these activities remain largely unexplored. To study used by effectors, we identified targets GpRbp-1 nematode Globodera pallida. Here, show...
The activity of intracellular plant nucleotide-binding leucine-rich repeat (NB-LRR) immune receptors is fine-tuned by interactions between the and their partners. Identifying NB-LRR interacting proteins therefore crucial to advance our understanding how these function. A co-immunoprecipitation/mass spectrometry screening was performed in Nicotiana benthamiana identify host associated with resistance protein Gpa2, a CC-NB-LRR receptor conferring against potato cyst nematode Globodera pallida....
To establish persistent infections in host plants, herbivorous invaders, such as root-knot nematodes, must rely on effectors for suppressing damage-induced jasmonate-dependent defenses. However, at present, the effector mechanisms targeting biosynthesis of biologically active jasmonates to avoid adverse responses are unknown. Using yeast two-hybrid, planta co-immunoprecipitation, and mutant analyses, we identified 12-oxophytodienoate reductase 2 (OPR2) an important target stylet-secreted...
Plant NLR proteins enable the immune system to recognize and respond pathogen attack. An early consequence of activation is transcriptional reprogramming. Some NLRs have been shown act in nucleus interact with transcription factors. The Rx1 protein potato binds distorts double-stranded DNA. However, components chromatin-localized complex are largely unknown. Here, we report a physical functional interaction between NbDBCP, bromodomain-containing chromatin-interacting protein. NbDBCP...
Lytic phages form a powerful platform for the display of large cDNA libraries and offer possibility to screen interactions with almost any substrate. To visualize these directly by fluorescence microscopy, we constructed fluorescent T7 exploiting flexibility incorporate modified versions its capsid protein. By applying translational frameshift sequences, helper plasmids were that expressed fixed ratio both wild-type protein (gp10) fused enhanced yellow (EYFP). The sequences inserted between...
ABSTRACT The Gpa2 and Rx1 intracellular immune receptors are canonical CC-NB-LRR proteins belonging to the same R gene cluster in potato. Despite sharing high sequence homology, they have evolved provide defence against unrelated pathogens. detects Gp-RBP-1 effectors secreted by potato cyst nematode Globodera pallida whereas recognizes viral coat protein (CP) of Potato Virus X (PVX). How perceive their matching remains unknown. Using a combination planta Co-Immunoprecipitation cellular...