A5101982917- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant-Microbe Interactions and Immunity
- Ubiquitin and proteasome pathways
- Nematode management and characterization studies
- Insect symbiosis and bacterial influences
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
- Protein Degradation and Inhibitors
- Kruppel-like factors research
- Monoclonal and Polyclonal Antibodies Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Metabolism, Diabetes, and Cancer
- Polysaccharides and Plant Cell Walls
- Studies on Chitinases and Chitosanases
- Osteoarthritis Treatment and Mechanisms
- Advanced biosensing and bioanalysis techniques
- Polymer Surface Interaction Studies
- Hydrogels: synthesis, properties, applications
- Plant Stress Responses and Tolerance
- RNA Interference and Gene Delivery
Hengyang Academy of Agricultural Sciences
2025
University of Idaho
2019-2024
First Hospital of China Medical University
2021-2023
China Medical University
2021-2023
Hefei University of Technology
2023
Center for Excellence in Molecular Plant Sciences
2017
Chinese Academy of Sciences
2017
University of Houston
2017
Air Force Medical University
2016
Xijing Hospital
2016
Plant pathogens, such as bacteria, fungi, oomycetes and nematodes, rely on wide range of virulent effectors delivered into host cells to suppress plant immunity. Although phytobacterial have been intensively investigated, little is known about the function plant-parasitic Globodera pallida, a cyst nematode responsible for vast losses in potato tomato industries. Here, we demonstrate using vivo vitro ubiquitination assays (Globodera pallida) effector RHA1B an E3 ubiquitin ligase that employs...
In plants, vegetative growth is controlled by synergistic and/or antagonistic effects of many regulatory factors. Here, the authors demonstrate that ubiquitin ligase seven in absentia1 (SINA1) mammalian BTF2-like transcription factors, Drosophila synapse-associated proteins, and yeast DOS2-like proteins (BSD1) function as a module to control tomato via regulation production plant hormone gibberellin (GA). SINA1 negatively regulates protein level BSD1 through ubiquitin-proteasome-mediated...
Plant-parasitic nematodes secrete effectors that manipulate plant cell morphology and physiology to achieve host invasion establish permanent feeding sites. Effectors from the highly expanded SPRYSEC (SPRY domain with a signal peptide for secretion) family in potato cyst have been implicated activation suppression of immunity, but mechanisms underlying these activities remain largely unexplored. To study used by effectors, we identified targets GpRbp-1 nematode Globodera pallida. Here, show...
The tomato transcription factor SlNAC1 plays an important role in abiotic stress response and is fine-tuned at both transcriptional posttranslational levels. gene strongly induced by multiple stresses the protein subjected to ubiquitin proteasome-mediated degradation. We found here that possesses two distinct transactivation domains (TADs), TAD1 TAD2. Significantly, instability of was attributed acidic amino acid-rich TAD1, which potential functionally overlapped; whereas glutamine-rich TAD2...
The insect cuticle works as the first line of defence to protect insects from pathogenic infections and water evaporation. However, old must be shed in order enter next developmental stage. During each ecdysis, moulting fluids are produced secreted into area among new cuticles. In a previous study, protein Bombyx mori single domain von Willebrand factor type C (BmSVWC; BGIBMGA011399) was identified Bo. demonstrated regulate ecdysis. this study we show that larvae, BmSVWC primarily locates...
Extensins are hydroxyproline-rich glycoproteins and generally play a structural role in cell wall integrity. In this study, we determined novel of tomato (Solanum lycopersicum) SENESCENCE-ASSOCIATED EXTENSIN1 (SAE1) leaf senescence. Both gain- loss-of-function analyses suggest that SAE1 plays positive senescence tomato. Transgenic plants overexpressing (SAE1-OX) exhibited premature enhanced dark-induced senescence, whereas knockout (SAE1-KO) displayed delayed development-dependent...
Abstract BSD (mammalian BTF2-like transcription factors, synapse-associated proteins, and DOS2-like proteins) is a conserved domain that exists in variety of organisms, but its function has not been well studied. Here, we identified novel domain-containing protein (SlBSD1) tomato (Solanum lycopersicum). Biochemical microscopy assays indicated SlBSD1 functional factor predominantly localized the nucleus. Loss-of-function overexpression analyses suggested regulator vegetative growth leaf...
Accumulating literature has suggested that hZIP1 and HIF-1α play vital roles in the tumor process of clear cell renal carcinoma (ccRCC). However, functional ccRCC remain largely unknown.HIF-1α protein level was evaluated by a western blot tissues lines. lines were transfected with HIF-1α-siRNA to downregulate expression HIF-1α. Then proliferative, migratory invasive abilities cells vitro detected real-time analysis (RTCA) assay, wound healing assay transwell respectively. The role vivo...
Gallbladder cancers (GBCs) are uncommon, but highly aggressive cancers. The majority of GBCs adenocarcinomas (ACs), rare subtypes such as squamous cell carcinoma (SC) and adenosquamous (ASC) observed well. clinicopathological characteristics SC/ASC have not been well documented. Expressions BIRC7 STC2 were in some tumors. However, expressions clinical significances gallbladder cancer reported.In this study, the protein 46 SCs/ASCs 80 ACs measured using immunohistochemistry. We demonstrated...
Studies on clear cell renal carcinoma (ccRCC) are gaining momentum due to its high malignancy and potential metastasize. F‑box protein 30 (FBXO30) is a member of the family; however, role mechanism in cancer remains be fully elucidated. Western blotting, reverse transcription‑quantitative PCR immunohistochemsitry were performed detect expression levels FBXO30 ccRCC tissues adjacent normal tissues. Tumor biological function assays animal experiments conducted clarify inhibitory effect...
Purpose: Accumulating evidence suggests that solute carrier family 39 member 1 (SLC39A1) conceivably function as a tumor suppressor, but the underlying mechanism in renal cell carcinoma (RCC) is poorly understood. Methods: OSRC-2 cancer cells were first transfected with SLC39A1 overexpressed vectors and empty then used transcriptomics, proteomics, metabolomics integrated analyses. Results: significantly altered several metabolisms at transcriptional, protein metabolic levels, including...
Ubiquitination, as a posttranslational modification of proteins, plays an important regulatory role in homeostasis eukaryotic cells. The covalent attachment 76 amino acid ubiquitin modifiers to target protein, depending on the length and topology polyubiquitin chain, can result different outcomes ranging from protein degradation changes localization and/or activity modified protein. Three enzymes sequentially catalyze ubiquitination process: E1 ubiquitin-activating enzyme, E2...
Objective To explore the relationship between hypermethylation and expression of cadherin 1(CDH1)with lung cancer.Methods The semi-quantitative real-time methylation specific polymerage chsin reaction(MSP)were used to detect promoter's relative ratio CDH1 in all 30 cases cancer tissues,para-cancer tissues,distal tissues 5 non-cancer tissue samples.Nested RT-PCR was mRNA.The western blot analysis E-cad protein.The immunohistoeheroical method confirm some negative results analysis.Results...