Toxic heavy metal elements in soils are major global environmental issues and easily migrate to crop grains cause severe problems human health, whereas moderately essential such as selenium beneficial for health. The accumulation of metals rice their genetic mechanisms still poorly understood. We conducted dissection four toxic (lead, cadmium, mercury, chromium), one quasi metallic element (arsenic), (selenium) 290 Xian 308 Geng accessions through a genome-wide association study (GWAS) based...

The rice-duck-crayfish (RDC) coculture system, an ecologically efficient breeding method, supports ducks' natural habits and enhances duck welfare. However, the optimal stocking density its influence on health in this system remains undetermined. study examined effects of densities growth, organ index, serum biochemistry, gut morphology microbiota ducks RDC system. A total five hundred forty 20-day-old Nonghu No. 2 were randomly divided into low-density (8 birds/666.67 m2, LD),...

ABSTRACT Calnexin, a calcium‐binding protein, promotes correct protein folding and prevents incompletely folded glycopolypeptides from premature oxidation degradation. Cryphonectria parasitica , an ascomycete fungus responsible for chestnut blight, poses significant threat to the forest or orchards worldwide. Although various aspects of calnexin have been investigated, little is known about impact fungal viruses. CpCne was identified characterized in this study, encoding C. . Strains with...

Platinum-based chemotherapy drugs play an indispensable role in clinical cancer treatment, but exhibit considerable side effects due to their non-specific mechanism of killing cells and normal cells. In this regard, the use antibodies conjugated anti-cancer platinum complexes will enable better differentiation from Here, six pincer-platinum(II) NHC (N-heterocyclic carbene) are reported, one which has amino group on N-alkyl ligand. This platinum(II) complex is used as payload for...

Osteosarcoma is the most common primary malignant bone tumor among children and young people associated with poor prognosis. Punicalagin an antioxidant ellagitannin found in pomegranate juice known antiproliferation anti‑angiogenesis properties. However, antitumor effect of punicalagin on osteosarcoma requires further investigation. In present study, inhibitory proliferation invasion was evaluated one human osteoblast cell line (hFOB1.19) three lines (U2OS, MG63 SaOS2). The cancer apoptosis...

Lung cancer is one of the leading malignancies worldwide, but regulatory mechanism its growth and metastasis still poorly understood. We investigated possible expression immunoglobulin G (IgG) genes in squamous cell carcinomas adenocarcinomas lung related lines. Abundant mRNA IgG essential enzymes for synthesis, recombination activation 1, 2 (RAG1, 2) activation-induced cytidine deaminase (AID) were detected cells not adjacent normal tissue or epithelial line. The extents 86 cancers found to...

Egg production is the most important economic trait in laying hens. To identify molecular markers and candidate genes associated with egg traits, such as age at first (AFE), weight (WFE), (EW), number (EN), maximum consecutive days (MCD), a genome-wide analysis by whole genome sequencing was performed Shuanglian chickens. Through quality control, total of 11,006,178 SNPs were obtained for further analysis. Heritability estimates ranged from moderate to high EW (0.897) MCD (0.632), low...

It was recently shown that resveratrol exerts neuroprotective effects against cerebral ischemia in mice. The aim of the present study to further confirm these vitro primary cortical neuron cultures with transient oxygen‑glucose deprivation (OGD), and investigate whether are due inhibition matrix metalloproteinase‑9 (MMP‑9) cell apoptosis. Neuronal cortex were prepared from BALB/c mice embryos (13‑15 days). Cells 14- 16-day subjected OGD for 3 h, followed by 21 h reoxygenation simulate...

The engagement of sexual behaviors is regulated by a number factors which include gene expression, hormone circulation, and multi-sensory information integration. In zebrafish, when male female are placed in the same container, they show mating-like regardless whether kept together or separated net. No observed same-sex animals put together. Through olfacto-visual centrifugal pathway, activation terminalis nerve olfactory bulb increases GnRH signaling brain triggers between males. zebrafish...

Chicken plumage color is an important economical trait in poultry breeding, as triple-yellow indigenous broilers are preferred over western commercial the Chinese market. However, studies on pigmentation of coloration relatively rare at present. Here, we performed a genome-wide mapping study F2 intercross, whose 2 founders were one hybrid line "High Quality chicken Line A" that originated from Anak red and "Huiyang Beard" classical "triple-yellow" breed. Moreover, used automatic colorimeter...

Abstract Various reports have indicated that a number of viruses could infect neutrophils, but the multiplication in neutrophils was abortive. Based on our previous finding avian influenza viral RNA and proteins were present nucleus infected human vivo , we investigated possibility 2009 A (H1N1) synthesis possible release infectious progeny from host cells. In this study found vitro without detectable level sialic acid expression be by virus strain. We also show can not only synthesize mRNA...

ERCC1, RRM1, TUBB3, TYMS and TOP2A genes have been shown to be associated with drug resistance in various types of tumors; however, their roles breast cancer chemotherapy not fully validated. In the present study, 140 well-matched patients cancer, comprising 70 receiving individualized classic chemotherapy, were analyzed. group, mRNA expression levels tissues measured using multiplex branched DNA liquidchip technology prior chemotherapy; an regimen was developed for each patient according...

Abstract MicroRNA (miR/miRNA) expression disorders play a crucial role in the development of gastric cancer (GC). Increasing evidence has indicated that miRNAs participate process numerous cancers. Previous research demonstrated miR-300 acts as cancer-promoting factor or tumor suppressor number tumors. However, to best our knowledge, effects on GC cells remain largely unknown. The present study investigated and analyzed its molecular mechanism. First, reverse transcription–quantitative...

Follicle development is a vital factor which determines the reproductive performance of poultry. Long noncoding RNAs (lncRNAs) have been reported to maintain animal function and play key roles in ovarian hormone secretion. But regulatory mechanism lncRNAs duck follicle has seldom reported. In this study, better explore molecular ducks, follicular lncRNA was sequenced analyzed. A total 9,551 were predicted follicles. Four hundred forty-five differentially expressed between white follicles...

Abstract The aim of the present study was to explore potential mechanism underlying involvement CB2 in osteoporosis. Micro‐CT utilized examine femur bone architecture. Also, real‐time PCR and Western blot analysis were detect effect 2‐AG on expression Notch, or interaction between Notch 2. treatment up‐regulated BMD, Tb.Sp SMI OVX mice, whereas proportion volume total (BV/TV), trabecular thickness (Tb.Th), number (Tb.N) mineral density (BMD) decreased 2‐AG‐treated mice. Accordingly,...

Influenza viruses possess a great threat to human health, but there is still no effective drug deal with the outbreak of possible new influenza subtypes. In this study, we first fractionated sialylated immunoglobulin G (IgG), mainly Fab fraction, sambucus nigra agglutinin affinity chromatography. We then demonstrated that IgG possessed more neutralizing activity against 2009 A (H1N1) subtype than mixture, and sialosides on crucial in neutralization reaction as when such residues were removed...

There is growing evidence showing that circular RNAs (circRNAs) are crucial regulators in modulating the biological behavior of tumors. This work aimed to probe role circ_0000517 non-small cell lung cancer (NSCLC) and elucidate its mechanism action.In this experimental study, differentially expressed circRNAs NSCLC were screened using GEO database (GSE158695). Circ_0000517, miR-326, miR-330-5p, MMP2 expression levels determined by quantitative real-time polymerase chain reaction (qRT-PCR)...

1. Measures of the bilateral apex pubis (BAP) in White Leghorn (WLH) hens aged from 16 to 64 weeks (from time ovaries were completely undeveloped senility) ranked into various groups so relationship between external traits (BAP distance, comb length, height, wattle body weight) and internal (largest ovarian follicle diameter (LFD) oviduct weight length) could be determined.2. Using correlation regression analysis with data age onset lay, measurements showed a strong LFD (r = 0.80, P < 0.01)...