A5015736102- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- Parasitic Diseases Research and Treatment
- Insect Resistance and Genetics
- Neurobiology and Insect Physiology Research
- Malaria Research and Control
- Vector-borne infectious diseases
- Aquaculture disease management and microbiota
- Silkworms and Sericulture Research
- Viral Infectious Diseases and Gene Expression in Insects
- Entomopathogenic Microorganisms in Pest Control
- Silk-based biomaterials and applications
- Insect Utilization and Effects
- Parasitic Infections and Diagnostics
- Parasite Biology and Host Interactions
- Antimicrobial Peptides and Activities
- Parasites and Host Interactions
- Insect Pest Control Strategies
- Insect and Arachnid Ecology and Behavior
- Yersinia bacterium, plague, ectoparasites research
- Helminth infection and control
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Parasitic infections in humans and animals
University of Missouri
2014-2024
Missouri College
2006-2021
Virginia Tech
2006
University of California, Irvine
1999-2005
University of Wisconsin–Madison
1989-1996
University of Wisconsin System
1994
Transgenic mosquitoes resistant to malaria parasites are being developed test the hypothesis that they may be used control disease transmission. We have an effector portion of antiparasite gene can resistance in transgenic mosquitoes. Mouse monoclonal antibodies recognize circumsporozoite protein Plasmodium gallinaceum block sporozoite invasion Aedes aegypti salivary glands. An anti-circumsporozoite antibody, N2H6D5, whose corresponding heavy- and light-chain variable regions were engineered...
The mosquito Armigeres subalbatus can encapsulate and kill > 80% of Brugia malayi microfilariae (mf) within 36 hr following ingestion. cascade biochemical events constituting this melanotic encapsulation response is also important in other biological events, including egg-chorion tanning. Certain entities, a tyrosine precursor, are thought to be shared among these activities. Because purported link, because the blood meal both initiates egg development source mf, we evaluated possibility...
Background Lepidoptera insects have a novel development process comprising several metamorphic stages during their life cycle compared with vertebrate animals. Unlike most that live on nectar the adult stage, Bombyx mori silkworm adults do not eat anything and die after egg-laying. In addition, midguts of produce antimicrobial proteins wandering stage when larval tissues undergo numerous changes. The exact mechanisms responsible for these phenomena remain unclear. Principal Findings We used...
Peptidoglycan recognition proteins (PGRPs) play essential roles in the immune systems of insects and higher animals against certain pathogens, including bacteria. In insects, most studies on functions PGRPs have been performed Drosophila, with only limited mosquitoes, which are important disease vectors. present study, we analysed PGRP sequences yellow fever mosquito, Aedes aegypti, acquired from two genome databases, identified a total seven genes; namely, PGRP-S1, -SC2, -LA, -LB, -LC, -LD...
Abstract Bursicon is a neuropeptide hormone consisting of two cystine-knot proteins (burs α and burs β), responsible for cuticle tanning other developmental processes in insects. Recent studies show that each bursicon subunit forms homodimers induce prophylactic immunity Drosophila melanogaster . Here, we investigated the hypothesis act insects, possibly arthropods, generally, using mosquito, Aedes aegypti We found β are expressed larvae, pupae newly emerged adults. Treating Ae. D. adults...
1 Donald Danforth Plant Science Center 975 N. Warson Rd, St. Louis, MO 63132, USA 2 Department of Cellular and Molecular Biology, The Ohio State University, Columbus, OH 43210, 3 Current address, Logos Procyte, 1005 Rd., Suite B01, St 4 Veterinary Pathobiology, University Missouri, Columbia, 65211, 5 New Mexico Consortium, Los Alamos National Laboratory, 4200 W Jemez Alamos, NM 87544,
Insects can be models for understanding human intestinal infection and pathology. Molting, a special period during which the old insect cuticle is shed new one produced, crucial development. Holometabolous insects may experience several larva-to-larva moltings to become larger, pupal molt adult eclosion adults. During larval molts, they stop feeding quiescent. Although molting larvae quiescent, it not known if changes in microbiome, physiology, development immunity of midguts...
The inherent ability of Brugia malayi and pahangi (Nematoda) to establish successful relationships with the mosquitoes Armigeres subalbatus Aedes aegypti Liverpool strain was evaluated. microfilariae (mff) avoided immune response developed normally in A. exposed parasite by an infective bloodmeal, whereas nearly 85% B. were destroyed response. Because supports development both filarial worm species but destroys intrathoracically inoculated isolated from jird blood, blood-isolated into...
The gene encoding sialokinin I, the principal vasodilatory peptide of Aedes aegypti, has been isolated and characterized. Degenerate oligonucleotide primers based on amino acid sequence were used to amplify a fragment from messenger RNA (mRNA) female salivary glands. amplification product was probe gland complementary DNA (cDNA) library, number corresponding cDNAs their primary determined. Analysis conceptual translation 406-bp cDNA indicates that I is expressed as preprosialokinin...
Insects depend on the innate immune response for defense against a wide array of pathogens. Central to Drosophila immunity are antimicrobial peptides (AMPs), released into circulation when pathogens trigger either two widely studied signal pathways, Toll or IMD. The pathway responds infection by Gram-positive bacteria and fungi while IMD is activated Gram-negative bacteria. During activation pathway, NF-κB-like transcription factor Relish phosphorylated then cleaved, which crucial...
Malaria affects over 200 million individuals annually, resulting in 800 000 fatalities. Current tests use blood smears and can only detect the disease when 0.1–1% of cells are infected. We investigating photoacoustic flowmetry to sense as few one infected cell among 10 or more normal cells, thus diagnosing infection before patients become symptomatic. Photoacoustic is similar conventional flow cytometry, except that rare targeted by nanosecond laser pulses induce ultrasonic responses. This...
Antibiotic treatment has emerged as a promising strategy to sterilize and kill filarial nematodes due their dependence on endosymbiotic bacteria, Wolbachia. Several studies have shown that novel FDA-approved antibiotics are efficacious at depleting the of endosymbiont, thus reducing female fecundity. However, it remains unclear if can permanently deplete Wolbachia cause sterility for lifespan adult worms. Concerns about resistance arising from mass drug administration necessitate careful...