A5039756363- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- CRISPR and Genetic Engineering
- Invertebrate Immune Response Mechanisms
- Malaria Research and Control
- Neurobiology and Insect Physiology Research
- Insect Utilization and Effects
- Insect and Pesticide Research
- Animal Genetics and Reproduction
- Viral Infectious Diseases and Gene Expression in Insects
- Vector-Borne Animal Diseases
- Chromosomal and Genetic Variations
- Viral Infections and Vectors
- Allergic Rhinitis and Sensitization
- Insect Pest Control Strategies
- Studies on Chitinases and Chitosanases
- Silkworms and Sericulture Research
- Insect-Plant Interactions and Control
- Food Allergy and Anaphylaxis Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Insect and Arachnid Ecology and Behavior
- Aquaculture disease management and microbiota
- Vector-borne infectious diseases
- Genetically Modified Organisms Research
University of California, Irvine
2015-2024
John Wiley & Sons (United States)
2019-2024
National Council on Family Relations
2019-2024
Hanover College
2022-2024
Liechtenstein Institute
2024
University of Maryland, College Park
2024
Middle East Institute
2024
Digital Scholar (United States)
2023
Virginia Tech
2023
University of North Carolina at Greensboro
2019-2022
Significance Malaria continues to impose enormous health and economic burdens on the developing world. Novel technologies proposed reduce impact of disease include introgression parasite-resistance genes into mosquito populations, thereby modifying ability vector transmit pathogens. Such have been developed for human malaria parasite Plasmodium falciparum . Here we provide evidence a highly efficient gene-drive system that can spread these antimalarial target population. This exploits...
Mosquitoes ( Aedes aegypti ) were genetically modified to exhibit impaired vector competence for dengue type 2 viruses (DENV-2). We exploited the natural antiviral RNA interference (RNAi) pathway in mosquito midgut by constructing an effector gene that expresses inverted-repeat (IR) derived from premembrane protein coding region of DENV-2 genome. The A. carboxypeptidase A promoter was used express IR epithelial cells after ingestion a bloodmeal. and inserted into genome white-eye Puerto Rico...
Dengue and dengue hemorrhagic fever are increasing public health problems with an estimated 50–100 million new infections each year. Aedes aegypti is the major vector of viruses in its range control this mosquito would reduce significantly human morbidity mortality. Present methods not sufficiently effective approaches needed urgently. A “sterile-male-release” strategy based on release mosquitoes carrying a conditional dominant lethal gene attractive methodology. Transgenic strains were...
Abstract Background Aedes aegypti mosquitoes are the main vectors of dengue viruses to humans. Understanding their biology and interactions with pathogen prerequisites for development transmission control strategies. Mosquito salivary glands organs involved directly in vertebrate hosts. Information on spatial distribution gene expression these is expected assist novel disease strategies, including those that entail release transgenic impaired vector competence. Results We report here...
Multiple stringent confinement strategies should be used whenever possible
The Asian tiger mosquito, Aedes albopictus, is a highly successful invasive species that transmits number of human viral diseases, including dengue and Chikungunya fevers. This has large genome with significant population-based size variation. complete sequence was determined for the Foshan strain, an established laboratory colony derived from wild mosquitoes southeastern China, region within historical range origin species. comprises 1,967 Mb, largest mosquito sequenced to date, its results...
An approach based on mosquitoes carrying a conditional dominant lethal gene (release of insects lethal, RIDL) is being developed to control the transmission dengue viruses by vector population suppression. A transgenic strain, designated OX3604C, major vector, Aedes aegypti , was engineered have repressible female-specific flightless phenotype. This strain circumvents need for radiation-induced sterilization, allows genetic sexing resulting in male-only releases, and permits release eggs...
A Cas9/guide RNA-based gene drive strain, AgNosCd-1, was developed to deliver antiparasite effector molecules the malaria vector mosquito, Anopheles gambiae The system targets cardinal ortholog producing a red-eye phenotype. Drive can achieve 98 100% in both sexes and full introduction observed small cage trials within 6 10 generations following single release of gene-drive males. No genetic load resulting from integrated transgenes impaired performance trials. Potential drive-resistant...
Abstract Cas9/gRNA-mediated gene-drive systems have advanced development of genetic technologies for controlling vector-borne pathogen transmission. These include population suppression approaches, analogs insecticidal techniques that reduce the number insect vectors, and modification (replacement/alteration) which interfere with competence to transmit pathogens. Here, we develop a recoded rescue system malaria vector, Anopheles stephensi , relieves load in females caused by integration...
The mariner transposable element is capable of interplasmid transposition in the embryonic soma yellow fever mosquito, Aedes aegypti . To determine if this demonstrated mobility could be utilized to genetically transform a modified marked with wild-type allele Drosophila melanogaster cinnabar gene was microinjected into embryos kynurenine hydroxylase-deficient, white-eyed recipient strain. Three 69 fertile male founders resulting from produced families colored-eyed progeny individuals,...
The mosquito Aedes aegypti is the world's most important vector of yellow fever and dengue viruses. Work currently in progress to control transmission these viruses by genetically altering capacity wild Ae. populations support virus replication. germ-line transformation system reported here constitutes a major advance toward implementation this strategy. A modified Hermes transposon carrying 4.7-kb fragment genomic DNA that includes wild-type allele Drosophila melanogaster cinnabar (cn) gene...
At a recent workshop, experts discussed the benefits, risks, and research priorities associated with using genetically manipulated insects in control of vector-borne diseases.
The saliva of hematophagous insects contains a variety pharmacologically active substances that counteract the normal hemostatic response to injury in vertebrate hosts. yellow-fever mosquito, Aedes aegypti, secretes an apyrase inhibits ADP-dependent platelet aggregation. Apyrase was purified as enzyme from adult female salivary glands and subjected tryptic digestion, resulting peptides were sequenced. amino acid sequences obtained match conceptual translation product cDNA clone isolated...
Abstract With their genome sequenced, Anopheles gambiae mosquitoes now serve as a powerful tool for basic research in comparative, evolutionary and developmental biology. The knowledge generated by these studies is expected to reveal molecular targets novel vector control pathogen transmission blocking strategies. Comparisons of gene‐expression profiles between adult male nonblood‐fed female revealed that roughly 22% the genes showed sex‐dependent regulation. Blood‐fed females switch...
Dengue fever is the most important arboviral disease world-wide, with Aedes aegypti being major vector. Interactions between mosquito host and dengue viruses (DENV) are complex vector competence varies among geographically-distinct Ae. populations. Additionally, caused by four antigenically-distinct viral serotypes (DENV1–4), each multiple genotypes. Each virus genotype interacts differently vertebrate invertebrate hosts. Analyses of alterations in transcriptional profiles during DENV...
Abstract Background Hematophagy is a common trait of insect vectors disease. Extensive genome-wide transcriptional changes occur in mosquitoes after blood meals, and these are related to digestive reproductive processes, among others. Studies expected reveal molecular targets for novel vector control pathogen transmission-blocking strategies. The mosquito Aedes aegypti (Diptera, Culicidae), Dengue viruses, Yellow Fever Virus (YFV) Chikungunya virus (CV), the subject this study look at gene...
Small laboratory cage trials of non-drive and gene-drive strains the Asian malaria vector mosquito, Anopheles stephensi, were used to investigate release ratios other strain properties for their impact on transgene spread during simulated population modification. We evaluated effects transgenes survival, male contributions next-generation populations, female reproductive success accumulation gene drive-resistant genomic target sites resulting from nonhomologous end-joining (NHEJ) mutagenesis...
Abstract Background Anopheles stephensi is the key vector of malaria throughout Indian subcontinent and Middle East an emerging model for molecular genetic studies mosquito-parasite interactions. The type form species responsible majority urban transmission across its range. Results Here, we report genome sequence annotation strain An. . 221 Mb assembly represents more than 92% entire was produced using a combination 454, Illumina, PacBio sequencing. Physical mapping assigned 62% onto...
Invasive species cause considerable ecological and economic damage. Despite decades of broad impacts invasives on diversity agriculture, the genetic adaptations near-term evolution invading populations are poorly understood. The fall webworm, Hyphantria cunea, a highly successful invasive that originated in North America, spread throughout Northern Hemisphere during past 80 years. Here, we use whole-genome sequencing transcriptome profiling to probe underlying bases for rapid adaptation this...