A5087190416- Mosquito-borne diseases and control
- Malaria Research and Control
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Research on Leishmaniasis Studies
- CRISPR and Genetic Engineering
- Genetic diversity and population structure
- Insect Pest Control Strategies
- Trypanosoma species research and implications
- Invertebrate Immune Response Mechanisms
- Evolution and Genetic Dynamics
- Neurobiology and Insect Physiology Research
- Parasites and Host Interactions
- Viral Infections and Vectors
- Toxin Mechanisms and Immunotoxins
- Vibrio bacteria research studies
- Insect-Plant Interactions and Control
- Vector-borne infectious diseases
- Animal Behavior and Reproduction
- Insect Utilization and Effects
- Dengue and Mosquito Control Research
- Animal Genetics and Reproduction
- Plant and animal studies
- Hemoglobinopathies and Related Disorders
- Insect and Pesticide Research
University of California, Davis
2016-2025
Vector Oncology (United States)
2006-2025
Vector (United States)
2008-2024
Universidade Nova de Lisboa
2023
Lee University
2023
University of Florida
1988-2023
University of California, Berkeley
2020
Bipar
2004-2013
Institut de Recherche pour le Développement
2012
Centre National de la Recherche Scientifique
2012
Culex quinquefasciatus (the southern house mosquito) is an important mosquito vector of viruses such as West Nile virus and St. Louis encephalitis virus, well nematodes that cause lymphatic filariasis. C. one species within the pipiens complex can be found throughout tropical temperate climates world. The ability to take blood meals from birds, livestock, humans contributes its pathogens between species. Here, we describe genomic sequence quinquefasciatus: Its repertoire 18,883...
Animal species adapt to changes in their environment, including man-made such as the introduction of insecticides, through selection for advantageous genes already present populations or newly arisen mutation. A possible alternative mechanism is acquisition adaptive from related via a process known introgression. Differing levels insecticide resistance between two African malaria vectors, Anopheles coluzzii and gambiae, have been attributed assortative mating species. In previous study, we...
Aedes aegypti is the principal mosquito vector for many arboviruses that increasingly infect millions of people every year. With an escalating burden infections and relative failure traditional control methods, development innovative measures has become paramount importance. The use gene drives sparked significant enthusiasm genetic mosquitoes; however, no such system been developed in Ae. aegypti. To fill this void, here we develop several CRISPR-based split vector. cleavage rates up to...
A Cas9/guide RNA-based gene drive strain, AgNosCd-1, was developed to deliver antiparasite effector molecules the malaria vector mosquito, Anopheles gambiae The system targets cardinal ortholog producing a red-eye phenotype. Drive can achieve 98 100% in both sexes and full introduction observed small cage trials within 6 10 generations following single release of gene-drive males. No genetic load resulting from integrated transgenes impaired performance trials. Potential drive-resistant...
Abstract Bloodfeeding arthropods transmit many of the world’s most serious infectious diseases. Leishmania are transmitted to their mammalian hosts when an infected sandfly probes in skin for a bloodmeal and injects parasite mixed with its saliva. Arthropod saliva contains molecules that affect blood flow modulate immune response host. Indeed, markedly enhances infectivity L. major If salivary molecule(s) responsible this phenomenon was identified, it might be possible vaccinate host against...
Significance Populations of the African malaria vector, Anopheles gambiae , are structured into M and S forms. All current work assumes two rarely hybridize. Here we show this assumption is false. We demonstrate ( i ) significant exchange genes between forms, even though ii hybrids have reduced fitness iii gene process spatially temporally dynamic. For malaria, it important to determine if for traits like insecticide resistance shared evolutionary biologists, confirms that mosquito a good...
Malaria transmission is dependent on the propensity of Anopheles mosquitoes to bite humans (anthropophily) instead other dead end hosts. Recent increases in usage Long Lasting Insecticide Treated Nets (LLINs) Africa have been associated with reductions highly anthropophilic and endophilic vectors such as gambiae s.s., leaving species a broader host range, arabiensis, most prominent remaining source many settings. An. arabiensis appears be more generalist terms its choice resting behavior,...
Abstract Despite significant reductions in malaria transmission across Africa since 2000, progress is stalling. This has been attributed to the development of insecticide resistance and behavioural adaptations vectors. Whilst widely investigated, there poorer understanding emergence, dynamics impact mosquito adaptations. We conducted a longitudinal investigation vector host choice over 3 years resting behaviour 4 following mass long-lasting insecticidal nets (LLINs) distribution Tanzania. By...
Abstract Anopheles gambiae populations in west Africa are complex, being composed of multiple, sympatric subpopulations. Recent studies have failed to reveal significant genetic differences among subpopulations, stimulating a debate regarding the levels gene flow them. The observed homogeneity may be consequence substantial contemporary or it that reproductive isolation is complete, but too recent for accumulation genic divergence. Here, we report results study estimating between An....
Chromosomal forms of Anopheles gambiae , given the informal designations Bamako, Mopti, and Savannah, have been recognized by presence or absence four paracentric inversions on chromosome 2 . Studies karyotype frequencies at sites where occur in sympatry led to suggestion that these represent species. We conducted a study genetic structure populations An from two villages Mali, west Africa. Populations each site were composed Bamako Mopti sibling species, arabiensis Karyotypes determined for...
"Lutzomyia longipalpis is a Species Complex: Genetic Divergence and Interspecific Hybrid Sterility among Three Populations" published on Jun 1993 by The American Society of Tropical Medicine Hygiene.
Leishmania donovani chagasi parasites, transmitted by sandflies of the Lutzomyia longipalpis species complex, normally cause visceral leishmaniasis. However, in Central America infections frequently result cutaneous disease. We undertook experiments to investigate possible influence sandfly saliva on course infection. Erythemas caused feeding correlated well with levels erythema-inducing peptide, maxadilan, their saliva. Saliva Brazilian flies was most potent, that Colombian less so, and...
The spread of insecticide resistance genes in Anopheles gambiae Giles sensu stricto threatens to compromise vector-based malaria control programs. Two mutations at the same locus voltage-gated sodium channel gene are known confer knockdown (kdr) pyrethroids and DDT. Kdr-e involves a leucine-serine substitution, it was until recently thought be restricted East Africa, whereas kdr-w, which leucine-phenylalanine is associated with West Africa. In this study, we analyze frequency relationship...
Abstract The population structure of the Anopheles gambiae complex is unusual, with several sibling species often occupying a single area and, in one these species, An. sensu stricto, as many three “chromosomal forms” occurring together. chromosomal forms are thought to be intermediate between populations and distinguishable by patterns chromosome gene arrangements. extent reproductive isolation among has been debated. To better characterize this we measured effective size, Ne, migration...
After transmission through the bite of female sand flies, Leishmania spp. can cause a broad spectrum disease manifestations collectively known as leishmaniases. L. amazonensis is endemic in South America, where it causes cutaneous, diffuse and visceral leishmaniasis. In this study, we have provided evidence that salivary gland extracts (SGE) Lutzomyia longipalpis enhances infection. BALB/c mice infected intradermally ear with 10(5) metacyclic promastigotes together SGE (equivalent to 0.5...
The spread of insecticide resistance genes in Anopheles gambiae Giles sensu stricto threatens to compromise vector-based malaria control programs. Two mutations at the same locus voltage-gated sodium channel gene are known confer knockdown (kdr) pyrethroids and DDT. Kdr-e involves a leucine-serine substitution, it was until recently thought be restricted East Africa, whereas kdr-w, which leucine-phenylalanine is associated with West Africa. In this study, we analyze frequency relationship...
In the Yucatán Peninsula of Mexico, main vector Chagas disease is Triatoma dimidiata. Field studies suggest that natural transmission occurs through transient and seasonal invasion houses by sylvatic/peridomestic triatomines, rather than persistent domiciliated bug populations. We investigated genetic structure T. dimidiata populations, using morphometry microsatellite markers, to assess dispersal individuals in this triatomine species understand dynamics domestic infestation. observed low...
The suggestion that genetic divergence can arise and/or be maintained in the face of gene flow has been contentious since first proposed. This controversy and a rarity good examples have limited our understanding this process. Partially reproductively isolated taxa highlighted as offering unique opportunities for identifying mechanisms underlying with flow. African malaria vector, Anopheles gambiae s.s., is widely regarded consisting two sympatric forms, thought by many to represent...
Abstract In certain cases, a species may have access to important genetic variation present in related via adaptive introgression. These novel alleles interact with their new background, resulting unexpected phenotypes. this study, we describe selective sweep on standing the X chromosome mosquito Anopheles coluzzii , principal malaria vector West Africa. This event been influenced by recent introgression of insecticide resistance gene known as kdr from sister gambiae . Individuals carrying...
In the summer of 2013, Aedes aegypti Linnaeus was first detected in three cities central California (Clovis, Madera and Menlo Park). It has now been multiple locations southern CA as far south San Diego Imperial Counties. A number published reports suggest that populations have established from independent introductions.Here we report population genomics analyses Ae. based on individual, field collected whole genome sequences. We analyzed 46 genomes to establish genetic relationships among...
We studied the larval distribution and composition of Anopheles arabiensis Patton, An. gambiae s.s. Giles, its forms, among local habitats; their association with adults between these habitats in Banambani village, Mali during mid-rainy seasons 1997-1999. For species form identification we used polymerase chain reaction (PCR) PCR-restriction fragment-length polymorphism (RFLP). Differences larvae were observed 1998, but not 1997 or 1999, although they on borderline statistical significance....
We compared microsatellite polymorphism at nine loci located on chromosome 3 among two colonies and a field population of Anopheles gambiae sensu stricto Giles mosquitoes. Numbers alleles observed each locus mean heterozygosities were drastically reduced laboratory colonies. Genetic analysis the used in this study revealed an unprecedented frequency rare (<0.05). In contrast, colony samples large numbers with frequencies >0.50. Partitioning data to assess impact alleles, null sample size...
Abstract Microsatellites are defined as tracts of tandemly repeated short DNA sequences. Polymorphisms in this class currently being used to generate a genetic map the mosquito Anopheles gambiae. In present study we explore potential microsatellites tool for studying structure natural populations malaria vector. Genetic polymorphism at twenty enzyme coding gene loci and eleven microsatellite was surveyed population An. gambiae from Mali, West Africa. All were polymorphic, compared 40%...