A5107443533- Malaria Research and Control
- Mosquito-borne diseases and control
- Insect Resistance and Genetics
- Genetic diversity and population structure
- Chromosomal and Genetic Variations
- African Botany and Ecology Studies
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Evolution and Genetic Dynamics
- Insect Pest Control Strategies
- Fish Biology and Ecology Studies
- Parasitic Diseases Research and Treatment
- Agriculture and Rural Development Research
- Identification and Quantification in Food
- Viral Infections and Vectors
- Trypanosoma species research and implications
- demographic modeling and climate adaptation
- Parasite Biology and Host Interactions
- Plant Pathogenic Bacteria Studies
- Invertebrate Immune Response Mechanisms
- Vibrio bacteria research studies
- Hemoglobinopathies and Related Disorders
- Genomics and Chromatin Dynamics
- Aquatic Ecosystems and Biodiversity
Istituto Pasteur
1998-2020
Sapienza University of Rome
1987-2019
University of Bamako
1998
Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale
1990
Centre Muraz
1990
Istituto Nazionale di Fisica Nucleare, Sezione di Roma I
1990
Abstract We karyotyped and identified by polymerase chain reaction restriction fragment length polymorphism (PCR‐RFLP) analysis Anopheles gambiae s.s. samples collected in several African countries. The data show the existence of two non‐panmictic molecular forms, named S M, whose distribution extended from forest to savannahs. Mosquitoes M forms are homosequential standard for chromosome‐2 inversions areas. In dry savannahs, is characterized mainly inversion polymorphisms typical Savanna...
Background The main constraint to the fight against container-breeding mosquito vectors of human arboviruses is difficulty in targeting multiplicity larval sources, mostly represented by small man-made water containers. aim this work assess feasibility "auto-dissemination" approach, already tested for Aedes aegypti, as a possible alternative traditional, inefficient control tools, Ae. albopictus urban areas. approach based on possibility that wild adult females, exposed artificial resting...
Chromosomal forms of Anopheles gambiae , given the informal designations Bamako, Mopti, and Savannah, have been recognized by presence or absence four paracentric inversions on chromosome 2 . Studies karyotype frequencies at sites where occur in sympatry led to suggestion that these represent species. We conducted a study genetic structure populations An from two villages Mali, west Africa. Populations each site were composed Bamako Mopti sibling species, arabiensis Karyotypes determined for...
Abstract Background The geographic and temporal distribution of M S molecular forms the major Afrotropical malaria vector species Anopheles gambiae s.s. at western extreme their range has never been investigated in detail. Materials methods Collections indoor-resting An. s.l. females were carried out along a ca. 400 km west to east transect following River Gambia from coastal region south-eastern Senegal during 2005 end rainy season/early dry season 2006 season. Specimens identified by...
In the Anopheles gambiae complex, paracentric chromosomal inversions are non-randomly distributed along complement: 18/31 (58%) of common polymorphic on chromosome arm 2R, which represents only ~30% complement. Moreover, in An. sensu stricto, 6/7 occur 2R. Most these considered markers ecological adaptation that increase fitness carriers alternative karyotypes contrasting habitats. However, little is known about evolutionary forces responsible for their origin and subsequent establishment...
Abstract Impacts of introgressive hybridisation may range from genomic erosion and species collapse to rapid adaptation speciation but opportunities study these dynamics are rare. We investigated the extent, causes consequences a hybrid zone between Anopheles coluzzii gambiae in Guinea-Bissau, where high rates appear be stable at least since 1990s. was genetically partitioned into inland coastal subpopulations, separated by central region dominated A. . Surprisingly, whole genome sequencing...
Anopheles arabiensis , one of the two most potent malaria vectors gambiae complex, is characterized by presence chromosomal paracentric inversions. Elucidation nature and dynamics these inversions paramount importance for understanding population genetics evolutionary biology this mosquito impact on epidemiology. We report here cloning breakpoints naturally occurring polymorphic inversion 2Rd′ A. . A cDNA clone that cytologically mapped proximal breakpoint was starting material isolation a...
Abstract In this work, Corbett-Detig et al. use proximity-ligation sequencing (Hi-C) of several Anopheles gambiae and A. coluzzii inversionbearing individuals to detect map inversion breakpoints. They show that breakpoints can be mapped precisely... Chromosomal inversions are fundamental drivers genome evolution. the main Afrotropical malaria vector species, belonging species complex, play an important role in local adaptation have a rich history cytological study. Despite importance...
Urban mosquitoes in temperate regions may represent a high nuisance and are associated with the risk of arbovirus transmission. Common practices to reduce this burden, at least Italian highly infested urban areas, imply calendar-based larvicide treatments street catch basins - which main non-removable breeding site and/or insecticide ground spraying. The planning these interventions, as well evaluation their effectiveness, rarely benefit adequate monitoring mosquito abundance dynamics. We...
The highly invasive mosquito species Aedes albopictus has become a major health concern in temperate areas due to its role as vector of exotic arboviruses. Pyrethroid insecticides represent the main tools for limiting circulation such mosquito-borne viruses. present work aim extend previous reports on phenotypic pyrethroid-resistance European Ae. albopictus, identify genetic basis and monitor geographical distribution resistant genotypes, with particular focus sites experiencing 2017...
Chromosomal inversion polymorphisms play an important role in adaptation to environmental heterogeneities. For mosquito species the Anopheles gambiae complex that are significant vectors of human malaria, paracentric abundant and associated with ecologically epidemiologically phenotypes. Improved understanding these traits relies on determining karyotype, which currently depends upon laborious cytogenetic methods whose application is limited both by requirement for specialized expertise...
Abstract The Anopheles gambiae complex of mosquitoes includes malaria vectors at different stages speciation, whose study enables a better understanding how adaptation to divergent environmental conditions leads evolution reproductive isolation. We investigated the population genetic structure closely related sympatric taxa that have recently been proposed as separate species ( An. coluzzii and ), sampled from diverse habitats along Gambia river in West Africa. characterized putatively...
Feasibility and costs of monitoring efforts aimed to monitor mosquito species are strictly dependent on the presence skilled entomologists directly in field. However, several contexts this is not possible or easy organize, thus limiting possibility obtain crucial information presence/abundance potential disease vectors new invasive species. Digital imaging approaches could be extremely useful frame medical entomology overcome limit. This work describes a surveillance approach collect...
Abstract. 1. Three sibling species of mosquitoes the Anopheles gambiae complex are found in Senegal River Basin: An.melas Theobald, An.gambiae Giles and An.arabiensis Patton. 2. is restricted to river delta environs where saltwater breeding places present. 3. sympatric study area; predominates coastal zones it breeds also during dry season; inland areas mostly rainy season (July‐September). 4. chromosomally polymorphic all over area, with much variation inversion frequencies, particularly...
Speciation with gene flow may be aided by reduced recombination helping to build linkage between genes involved in the early stages of reproductive isolation. Reduced on chromosome X has been implicated speciation within Anopheles gambiae complex, species which represent major Afrotropical malaria vectors. The most recently diverged, morphologically indistinguishable, pair, A. and coluzzii, ubiquitously displays a 'genomic island divergence' spanning over 4 Mb from centromere, represents...
Abstract Anopheles melas is a brackish water–breeding member of the gambiae complex that distributed along coast West Africa and major malaria vector within its range. Because little known about population structure this species, we analysed 15 microsatellite markers 1161 bp mt DNA in 11 A. populations collected throughout Compared with sibling species , have high level genetic differentiation between them, representing patchy distribution due to fragmented larval habitat associated...
Abstract Background The malaria vector Anopheles gambiae is polymorphic for chromosomal inversions on the right arm of chromosome 2 that segregate nonrandomly between assortatively mating populations in West Africa. One such inversion, 2Rj, associated with BAMAKO form endemic to southern Mali and northern Guinea Conakry near Niger River. Although it exploits a unique ecology both molecular data suggest reduced gene flow other A. populations, no markers exist identify this form. Methods To...
Chromosomal inversion polymorphisms play a role in adaptation to heterogeneous environments. Inversion are implicated the very high ecological flexibility of three main malaria vector species Afrotropical Anopheles gambiae complex, facilitating exploitation anthropogenic environmental modifications and promoting strong association with humans. In addition extending species' spatial temporal distribution, inversions associated epidemiologically relevant mosquito behavior physiology,...
Abstract Chromosomal inversions are fundamental drivers of genome evolution. In the main afro-tropical malaria vector species, belonging to Anopheles gambiae species complex, play an important role in local adaptation and have a rich history cytological study. Despite importance ubiquity some chromosomal across inversion breakpoints often challenging map molecularly due presence large repetitive regions. Here, we develop approach that uses Hi-C sequencing data fine-map 2Rbc 2Rd A. coluzzii ....