A5009042229- Mosquito-borne diseases and control
- Insect Resistance and Genetics
- Insect Pest Control Strategies
- Malaria Research and Control
- Viral Infections and Vectors
- Insect and Pesticide Research
- Dermatological diseases and infestations
- Insect symbiosis and bacterial influences
- Insect-Plant Interactions and Control
- Vector-borne infectious diseases
- Dengue and Mosquito Control Research
- Vector-Borne Animal Diseases
- Insects and Parasite Interactions
- Cholinesterase and Neurodegenerative Diseases
- Forensic Entomology and Diptera Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Bartonella species infections research
- Animal Ecology and Behavior Studies
- Yersinia bacterium, plague, ectoparasites research
- Amino Acid Enzymes and Metabolism
- Insect Utilization and Effects
- Computational Drug Discovery Methods
- Bird parasitology and diseases
- bioluminescence and chemiluminescence research
- Neurobiology and Insect Physiology Research
National Institute of Infectious Diseases
2016-2025
University of Niigata Prefecture
2022
Cornell University
2000-2019
Jiangxi University of Science and Technology
2019
Jiaxing University
2019
Cardiff University
2019
Indian Institute of Chemical Technology
2019
The University of Texas at Arlington
2019
Florida Gulf Coast University
2019
Universidad de Guanajuato
2019
Adult house flies, Musca domestica L., are mechanical vectors of more than 100 devastating diseases that have severe consequences for human and animal health. House fly larvae play a vital role as decomposers wastes, thus live in intimate association with many pathogens. We sequenced analyzed the genome using DNA from female flies. The is 691 Mb. Compared Drosophila melanogaster, contains rich resource shared novel protein coding genes, significantly higher amount repetitive elements,...
Aedes aegypti is the major vector of yellow and dengue fevers. After 10 generations adult selection, an A. strain (SP) developed 1650-fold resistance to permethrin, which one most widely used pyrethroid insecticides for mosquito control. SP larvae also 8790-fold following selection adults. Prior selections, frequencies V1016G F1534C mutations in domains II III, respectively, voltage-sensitive sodium channel (Vssc, target site insecticide) were 0.44 0.56, respectively. In contrast, only G1016...
The voltage-sensitive sodium (Na+) channel (Vssc) is the target site of pyrethroid insecticides. Pest insects develop resistance to this class insecticide by acquisition one or multiple amino acid substitution(s) in channel. In Southeast Asia, two major Vssc types confer dengue mosquito vector Aedes aegypti, namely, S989P+V1016G and F1534C. We expressed several Xenopus oocytes examined effect substitutions on susceptibilities. F1534C haplotypes reduced sensitivity permethrin 100- 25-fold,...
Background Resistance of Aedes aegypti to photostable pyrethroid insecticides is a major problem for disease-vector control programs. Pyrethroids target the voltage-gated sodium channel on insects' neurons. Single amino acid substitutions in this associated with resistance are one main factors that cause knockdown insects. Although kdr has been observed several mosquito species, point mutations para gene have not fully characterized Ae. populations Vietnam. The aim study was determine types...
The Aedes mosquito, which transmits the dengue fever virus and other viruses, has acquired resistance to pyrethroid insecticides in a naturally selective manner. Massive use of led worldwide expansion resistant populations. major factor is knockdown (kdr) caused by amino acid mutation(s) voltage-gated sodium channel, target site this insecticide group. Some kdr mutations can lead dramatic increase resistance, multiple level 10 several-hundred. In review, we summarize identified mosquitoes...
Anopheles gambiae s.s., arabiensis, and funestus s.s. are the most important species for malaria transmission. Pyrethroid resistance of these vector mosquitoes is one main obstacles against effective control. The objective present study was to monitor pyrethroid susceptibility in 3 major vectors a highly endemic area western Kenya elucidate mechanisms species. Gembe East West, Mbita Division, 4 islands Suba district Nyanza province were used as area. Larval adult collection bioassay...
Abstract Recently-emerging genome editing technologies have enabled targeted gene knockout experiments even in non-model insect species. For studies on insecticide resistance, offer some advantages over the conventional reverse genetic technique, RNA interference, for testing causal relationships between genes of detoxifying enzymes and resistance phenotypes. There were relatively abundant evidences indicating that overexpression a cytochrome P450 CYP9M10 confers strong pyrethroid larvae...
IntroductionAedes albopictus (Skuse) is an important vector of arboviral diseases, including dengue, chikungunya and Zika virus disease. Monitoring insecticide resistance mechanisms by which the mosquito develops crucial to minimise disease transmission.AimTo determine status in Ae. from different geographical regions.MethodsWe sampled 33 populations Asia, Europe South America, tested these for susceptibility permethrin, a pyrethroid insecticide. In resistant populations, target site...
Abstract Background Mosquito control is a crucial global issue for protecting the human community from mosquito-borne diseases. There an urgent need development of selective and safe reagents mosquito control. Flavonoids, group chemical substances with variable phenolic structures, such as daidzein, have been suggested potential larvicides less risk to environment. However, mode larvicidal action flavonoids has not elucidated. Results Here, we report that several flavonoids, including...
Aedes aegypti (Linnaeus, 1762) is the main mosquito vector for dengue and other arboviral infectious diseases. Control of this important highly relies on use insecticides, especially pyrethroids. The high frequency (>78%) L982W substitution was detected at target site pyrethroid insecticide, voltage-gated sodium channel (Vgsc) A. collected from Vietnam Cambodia. Alleles having concomitant mutations + F1534C V1016G were also confirmed in both countries, their (>90%) Phnom Penh, Strains these...
ABSTRACT The symbiotic bacteria associated with the stomach disc, a large aggregate of bacteriocytes on ventral side midgut, human body and head lice were characterized. Molecular phylogenetic analysis 16S rRNA gene sequences showed that symbionts formed distinct well-defined clade in Gammaproteobacteria . exhibited AT-biased nucleotide composition accelerated molecular evolution. In situ hybridization revealed nymphs adult males, symbiont was localized while females, not disc but lateral...
To evaluate the vectorial capacity of mosquitoes for viruses in Japan, host-feeding habits were analyzed by sequencing polymerase chain reaction-amplified fragments cytochrome b and 16S ribosomal RNA regions mitochondrial DNA 516 15 species from seven genera that collected residential areas during 2003-2006. Culex pipiens L. Aedes albopictus Skuse most commonly urban suburban areas. Anautogenous pallens Coquillett was distinguished autogenous Cx. form molestus Forskal using a reaction-based...
Aedes aegypti is a vector of several important human pathogens. Control efforts rely primarily on pyrethroid insecticides for adult mosquito control, especially during disease outbreaks. A. has developed resistance nearly everywhere it occurs and are used. An mechanism due to mutations in the voltage-sensitive sodium channel (Vssc) gene. Two mutations, particular, S989P + V1016G, commonly occur together parts Asia.We have created strain (KDR:ROCK) that contains Vssc V1016G as only within...
Jingmen tick virus (JMTV) and the related jingmenvirus-termed Alongshan are recognized as globally emerging human pathogenic tick-borne viruses. These viruses have been detected in various mammals invertebrates, although their natural transmission cycles remain unknown. JMTV a novel jingmenvirus, tentatively named Takachi (TAKV), now identified during surveillance of Japan. was shown to be distributed across extensive areas Japan has repeatedly at same collection sites over several years,...
Background Aedes aegypti transmits various arthropod-borne diseases such as dengue, posing a significant burden to public health in tropical and subtropical regions. Pyrethroid-based control strategies are effective managing this vector; however, the development of insecticide resistance has hindered these efforts. Hence, long-term monitoring mosquito populations is crucial for vector disease control. Methodology/principal findings In study, we identified due voltage-gated sodium channel (...
Abstract Understanding how life is adapting to urban environments represents an important challenge in evolutionary biology. Here we investigate a widely cited example of adaptation, Culex pipiens form molestus , also known as the London Underground Mosquito. Population genomic analysis ∼350 contemporary and historical samples counter popular hypothesis that originated belowground less than 200 years ago. Instead, show first adapted human aboveground Middle East over course >1000 years,...
CYP6D1 is a house fly cytochrome P450 that catalyzes metabolism of pyrethroid insecticides. Overexpression responsible for monooxygenase-mediated resistance in the LPR (Learn resistant) strain, which was originally collected New York State. To determine whether involved other populations, we obtained two pyrethroid-resistant strains from Georgia and investigated relationship between piperonyl butoxide suppressible permethrin gene expression. The poultry facility about 1983, showed no PBO not...