A5046856926- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Trypanosoma species research and implications
- Insect symbiosis and bacterial influences
- Research on Leishmaniasis Studies
- Morinda citrifolia extract uses
- Bioactive Compounds and Antitumor Agents
- Dengue and Mosquito Control Research
- Vector-Borne Animal Diseases
- Malaria Research and Control
- Insect Resistance and Genetics
- Vector-borne infectious diseases
- Pharmacological Effects of Natural Compounds
- Synthesis and Biological Evaluation
- Moringa oleifera research and applications
- Phytochemical compounds biological activities
- Crystallography and molecular interactions
- Insect Pest Control Strategies
- Crystallization and Solubility Studies
- Viral Infections and Outbreaks Research
- Insect and Pesticide Research
- X-ray Diffraction in Crystallography
- Plant Virus Research Studies
- Phytochemistry and Biological Activities
- Insect-Plant Interactions and Control
University of Tennessee at Knoxville
2021-2023
National Institute of Infectious Diseases
2019-2022
Noguchi Memorial Institute for Medical Research
2015-2021
University of Ghana
2015-2021
Tokyo Medical and Dental University
2017-2021
Kyushu University
2021
Japanese encephalitis (JE) remains a public health concern in several countries, and the Culex mosquito plays central role its transmission cycle. mosquitoes harbor wide range of viruses, including insect-specific viruses (ISVs), can transmit variety arthropod-borne (arboviruses) that cause human animal diseases. The current trend studies displays enhanced efforts to characterize virome through bulk RNA sequencing due possible arbovirus–ISV interactions; however, extent viral diversity taxon...
Aedes aegypti (Linnaeus, 1762) is the main mosquito vector for dengue and other arboviral infectious diseases. Control of this important highly relies on use insecticides, especially pyrethroids. The high frequency (>78%) L982W substitution was detected at target site pyrethroid insecticide, voltage-gated sodium channel (Vgsc) A. collected from Vietnam Cambodia. Alleles having concomitant mutations + F1534C V1016G were also confirmed in both countries, their (>90%) Phnom Penh, Strains these...
Trypanosomiasis, leishmaniasis, and malaria are protozoan infections of public health importance with thousands new cases recorded annually. Control these infection(s) existing chemotherapy is limited by drug toxicity, lengthy parenteral treatment, affordability, and/or the emergence resistant strains. Medicinal plants on other hand used in treatment various infectious diseases although their chemical properties not fully evaluated. In this study, we screened 112 crude extracts from 72...
Trypanosoma brucei parasites are kinetoplastid protozoa that devastate the health and economic well-being of millions people in Africa through disease human African trypanosomiasis (HAT). New chemotherapy has been eagerly awaited due to severe side effects drug resistance issues plaguing current drugs. Recently, there an emphasis on use medicinal plants worldwide. Morinda lucida Benth. is a popular plant widely distributed Africa, several research groups have reported antiprotozoal...
Japanese encephalitis virus (JEV) is maintained in an enzootic cycle between swine, water birds, and mosquitoes. JEV has circulated indigenously Asia, with Culex tritaeniorhynchus as the primary vector. In some areas where vector scarce or absent, sporadic cases of have been reported, Aedes japonicus presumed to potential a secondary As one world’s most invasive culicid species, Ae . j carries considerable health risk for spreading diseases wider areas, including Europe North America. Thus,...
Entomological surveillance is one of the tools used in monitoring and controlling vector-borne diseases. However, use entomological for arboviral infection vector control often dependent on finding infected individuals. Although this method may suffice highly endemic areas, it not as effective spread diseases low non-endemic areas. In study, we examined efficiency using markers to assess status risk arbovirus Ghana, which considered a country, by combining mosquito with virus isolation...
Most developing countries resort to medicinal plants for treating diseases, but few of these have scientific backing their use. The aim the study was validate traditional use Morinda lucida leaves in inflammation and determine mechanism action.Effect hydroethanolic leaf extract M. (HEML) on localized evaluated using rat paw edema presented by sub-planter injections λ-carrageenan, histamine or serotonin separate experiments. Systemic lipopolysaccharide (LPS)-induced hyperthermia. Antioxidant...
Late male-killing, a male-specific death after hatching, is unique phenotype found in Homona magnanima , oriental tea tortrix. The male-killing agent was suspected to be an RNA virus, but details were unknown. We herein successfully isolated and identified the putative virus as Osugoroshi viruses (OGVs). three RNA-dependent polymerase genes detected phylogenetically related Partitiviridae, group of segmented double-stranded viruses. Purified dsRNA from late strain H. revealed 24 segments,...
Japanese encephalitis virus (JEV) is transmitted between swine, migratory birds, and Culex mosquitoes, has circulated indigenously in Asia for almost a century. Despite being the country with highest JEV diversity, surveillance targeting of Indonesia's vectors scarce. This study collected mosquitoes from several locations Tabanan Regency, Bali Island, Indonesia. We captured classified 3,032 adult into seven species, vishnui subgroup making up approximately 90% total. was identified by...
Abstract Background Dengue virus (DENV) is a mosquito-borne arbovirus transmitted by Aedes mosquitoes, but not endemic in all areas where this vector found. For example, the relatively sparse distribution of cases West Africa generally attributed to refractory nature African aegypti ( Ae. ) DENV infection, and particularly forest-dwelling Ae . formosus However, recent studies have shown these mosquitoes be competent vectors within some countries that suffered outbreaks past, such as Senegal....
Leishmaniasis is an infectious disease transmitted by the sand fly. It caused over 20 different species of Leishmania and has affected 14 million people worldwide. One main forms control leishmaniasis chemotherapy, but this limited high cost and/or toxicity available drugs. We previously found three novel compounds with iridoid tetracyclic skeleton to have activity against trypanosome parasites. In study, we determined anti-trypanosome using field strain, 010, lab strain hertigi. The minimum...
Abstract Tabanid flies (Tabanidae: Diptera) are common hematophagous insects known to transmit some pathogens mechanically or biologically animals; they widely distributed throughout the world. However, no tabanid-borne viruses, except transmitted have been reported date. In this study, we conducted RNA virome analysis of several human-biting tabanid species in Japan, discover and characterize viruses associated with tabanids. A novel flavivirus was encountered during study Japanese horse...
Abstract Background The Getah virus (GETV) is a mosquito-borne Alphavirus (family Togaviridae ) that of significant importance in veterinary medicine. It has been associated with major polyarthritis outbreaks animals, but there are insufficient data on its clinical symptoms humans. Serological evidence GETV exposure and the risk zoonotic transmission makes potentially medically relevant arbovirus. However, minimal emphasis placed investigating vector transmission, which limits current...
African trypanosomiasis is a disease caused by the parasitic protozoa of
The development of sequencing technologies, in recent years, gives novel insights into the diversity viruses arthropods. Human pathogenic or possible arthropod-borne (arboviruses) including from mosquitoes and ticks have been found by RNA virome analysis using a high-throughput sequencer. However, studies for other blood-sucking arthropods like biting midges as well black flies are relatively scarce. In this study, to find hematophagous Chironomoidea flies, we performed analyses field-caught...
Visceral leishmaniasis (VL) is a major problem worldwide and causes significant morbidity mortality. Existing drugs against VL have limitations, including their invasive means of administration long duration treatment regimens. There are also concerns regarding increasing relapses as well the identification resistant clinical strains with use miltefosine, sole oral drug for VL. is, therefore, an urgent need new alternative In present study, we show leishmanicidal effect novel, antimalarial...
Trypanosoma are known to be a diverse group of parasites that infect animals belonging all classes in the subphylum Vertebrata and important pathogens affect human animal health. Although many trypanosomatids have been found mammals birds Japan, information regarding their invertebrate host is currently lacking. During our virome analyses ticks horse flies, several trypanosoma-like sequences were found. Further sequence characterization PCR-based screening revealed termed sp. 17ISK-T2...
Abstract Objective To generate monoclonal antibodies ( MA bs) for developing a rapid malaria diagnostic urine‐based assay RUBDA ), using Plasmodium‐ infected human urinary antigens. Methods Plasmodium ‐infected PA g HU ) and cultured parasite CP fAg) antigens were used to mouse bs. The reactivity accuracy of the bs produced then evaluated microplate ELISA , SDS ‐ PAGE Western blotting assay, microscopy immunochromatographic tests. Results Ninety‐six b clones generated, which 68.8% reacted...
African trypanosomiasis poses serious health and economic concerns to humans livestock in several sub-Saharan countries. The aim of the present study was identify antitrypanosomal compounds from