A5074792204- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Vector-Borne Animal Diseases
- Dengue and Mosquito Control Research
- Vector-borne infectious diseases
- Viral Infections and Outbreaks Research
- Zoonotic diseases and public health
- Malaria Research and Control
- Plant Virus Research Studies
- Identification and Quantification in Food
- Trypanosoma species research and implications
National Institute of Infectious Diseases
2019-2024
The University of Tokyo
2020-2021
Japanese encephalitis (JE) remains a public health concern in several countries, and the Culex mosquito plays central role its transmission cycle. mosquitoes harbor wide range of viruses, including insect-specific viruses (ISVs), can transmit variety arthropod-borne (arboviruses) that cause human animal diseases. The current trend studies displays enhanced efforts to characterize virome through bulk RNA sequencing due possible arbovirus–ISV interactions; however, extent viral diversity taxon...
Jingmen tick virus (JMTV) and the related jingmenvirus-termed Alongshan are recognized as globally emerging human pathogenic tick-borne viruses. These viruses have been detected in various mammals invertebrates, although their natural transmission cycles remain unknown. JMTV a novel jingmenvirus, tentatively named Takachi (TAKV), now identified during surveillance of Japan. was shown to be distributed across extensive areas Japan has repeatedly at same collection sites over several years,...
Japanese encephalitis virus (JEV) is maintained in an enzootic cycle between swine, water birds, and mosquitoes. JEV has circulated indigenously Asia, with Culex tritaeniorhynchus as the primary vector. In some areas where vector scarce or absent, sporadic cases of have been reported, Aedes japonicus presumed to potential a secondary As one world’s most invasive culicid species, Ae . j carries considerable health risk for spreading diseases wider areas, including Europe North America. Thus,...
Entomological surveillance is one of the tools used in monitoring and controlling vector-borne diseases. However, use entomological for arboviral infection vector control often dependent on finding infected individuals. Although this method may suffice highly endemic areas, it not as effective spread diseases low non-endemic areas. In study, we examined efficiency using markers to assess status risk arbovirus Ghana, which considered a country, by combining mosquito with virus isolation...
In Asia, Culex mosquitoes are of particular interest because their role in maintaining endemic mosquito-borne viral diseases, including the Japanese encephalitis virus (JEV). Nonetheless, host-feeding preferences, along with naturally infecting RNA viruses certain species, remain understudied. this study, selected blood-fed were processed for avian and mammalian blood meal source identification. Concurrently, cell culture propagation high-throughput sequencing (HTS) approaches used to...
Japanese encephalitis virus (JEV) is transmitted between swine, migratory birds, and Culex mosquitoes, has circulated indigenously in Asia for almost a century. Despite being the country with highest JEV diversity, surveillance targeting of Indonesia's vectors scarce. This study collected mosquitoes from several locations Tabanan Regency, Bali Island, Indonesia. We captured classified 3,032 adult into seven species, vishnui subgroup making up approximately 90% total. was identified by...
Abstract Background Dengue virus (DENV) is a mosquito-borne arbovirus transmitted by Aedes mosquitoes, but not endemic in all areas where this vector found. For example, the relatively sparse distribution of cases West Africa generally attributed to refractory nature African aegypti ( Ae. ) DENV infection, and particularly forest-dwelling Ae . formosus However, recent studies have shown these mosquitoes be competent vectors within some countries that suffered outbreaks past, such as Senegal....
During their blood-feeding process, ticks are known to transmit various viruses vertebrates, including humans. Recent viral metagenomic analyses using next-generation sequencing (NGS) have revealed that arthropods like harbor a large diversity of viruses. However, many these not been isolated or cultured, and basic characteristics remain unknown. This study aimed present the identification difficult-to-culture virus in NGS understand its epidemic dynamics molecular biology techniques....
Abstract Tabanid flies (Tabanidae: Diptera) are common hematophagous insects known to transmit some pathogens mechanically or biologically animals; they widely distributed throughout the world. However, no tabanid-borne viruses, except transmitted have been reported date. In this study, we conducted RNA virome analysis of several human-biting tabanid species in Japan, discover and characterize viruses associated with tabanids. A novel flavivirus was encountered during study Japanese horse...
Abstract Background The Getah virus (GETV) is a mosquito-borne Alphavirus (family Togaviridae ) that of significant importance in veterinary medicine. It has been associated with major polyarthritis outbreaks animals, but there are insufficient data on its clinical symptoms humans. Serological evidence GETV exposure and the risk zoonotic transmission makes potentially medically relevant arbovirus. However, minimal emphasis placed investigating vector transmission, which limits current...
Abstract Background Dengue fever, caused by the dengue virus (DENV), is most common viral infection transmitted Aedes mosquitoes (mainly Ae. aegypti and albopictus ) worldwide. not currently established in Japan, primary vector mosquito for DENV country, but knowledge of its susceptibility limited. Therefore, we aimed to clarify status comparing dissemination dynamics Japanese all known serotypes with those . Methods After propagation each serotype Vero cells, culture supernatants were mixed...
Japanese encephalitis virus (JEV) constitutes a persistent public health concern in Japan. Despite the prevalence of potential JEV vectors, Aedes albopictus Skuse and Culex pipiens form molestus Forskål, their competence within mosquito population remains unexplored. This study aimed to assess role these two species, incorporating an analysis insect-specific (ISV) infection status comprehensively understand vector assessment. Mosquito colonies were initially screened by high-throughput...
The development of sequencing technologies, in recent years, gives novel insights into the diversity viruses arthropods. Human pathogenic or possible arthropod-borne (arboviruses) including from mosquitoes and ticks have been found by RNA virome analysis using a high-throughput sequencer. However, studies for other blood-sucking arthropods like biting midges as well black flies are relatively scarce. In this study, to find hematophagous Chironomoidea flies, we performed analyses field-caught...
Genotype IV Japanese encephalitis (JE) virus (GIV JEV) is the least common and most neglected genotype in JEV. We evaluated growth pathogenic potential of GIV strain 19CxBa-83-Cv, which was isolated from a mosquito pool Bali, Indonesia, 2019, serological analyses were also conducted. The ability 19CxBa-83-Cv Vero cells intermediate between that I (GI) Mie/41/2002 V (GV) Muar, whereas grew faster than Muar mouse neuroblastoma cells. neuroinvasiveness mice higher but lower Muar; however, there...
Trypanosoma are known to be a diverse group of parasites that infect animals belonging all classes in the subphylum Vertebrata and important pathogens affect human animal health. Although many trypanosomatids have been found mammals birds Japan, information regarding their invertebrate host is currently lacking. During our virome analyses ticks horse flies, several trypanosoma-like sequences were found. Further sequence characterization PCR-based screening revealed termed sp. 17ISK-T2...
Japanese encephalitis virus (JEV), a mosquito-borne virus, causes severe clinical symptoms in humans the Asian-Pacific region, where it circulates primary transmission cycle among Culex tritaeniorhynchus mosquitoes, domestic swine (Sus scrofa domesticus), and wading birds. We report here an anomalous result that JEV was detected unfed host-questing ticks collected from field Japan. genomic RNA four pools of Haemaphysalis flava nymphs November December 2019, March 2020, when Cx. adults were...
Abstract Arthropod-derived cell lines serve as crucial tools for studying arthropod-borne viruses (arboviruses). However, it has recently come to light that certain harbor persistent infections of arthropod-specific viruses, which do not cause any apparent cytopathic effects. Moreover, some these viral either inhibit or promote the growth arboviruses. Therefore, is utmost importance identify presence such and understand their impact on arboviral infections. In this study, we conducted a...
Japanese encephalitis virus (JEV) genotype IV (GIV) is one of the least common and most neglected genotypes worldwide, having been identified only on a few Indonesian islands until it was recently found to be cause outbreaks that occurred in several Australian states early 2022. Given limited availability information, vector range for JEV GIV remains unknown; thus, understanding this could prove invaluable future prevention efforts new areas. Herein, we experimentally exposed four mosquito...