A5101483465- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Dengue and Mosquito Control Research
- Insect Pest Control Strategies
- Bird parasitology and diseases
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Insect-Plant Interactions and Control
- Insects and Parasite Interactions
- Zoonotic diseases and public health
- Animal Ecology and Behavior Studies
- Avian ecology and behavior
- Forensic Entomology and Diptera Studies
- Study of Mite Species
- Insect behavior and control techniques
- Insect and Arachnid Ecology and Behavior
- Parasite Biology and Host Interactions
- Vibrio bacteria research studies
- Plant and animal studies
- Ecology and Vegetation Dynamics Studies
- Identification and Quantification in Food
National Institute of Infectious Diseases
2016-2025
Tottori University
2021-2024
Nagasaki University
1998-2021
National Institute for Japanese Language and Linguistics
2021
St. Marianna University School of Medicine
2021
University of Notre Dame
2016
National Institutes of Health
2006-2015
Inha University
2008
Swedish University of Agricultural Sciences
2002
Okayama University
1982-1985
Japanese encephalitis (JE) remains a public health concern in several countries, and the Culex mosquito plays central role its transmission cycle. mosquitoes harbor wide range of viruses, including insect-specific viruses (ISVs), can transmit variety arthropod-borne (arboviruses) that cause human animal diseases. The current trend studies displays enhanced efforts to characterize virome through bulk RNA sequencing due possible arbovirus–ISV interactions; however, extent viral diversity taxon...
A larval survey of dengue vectors was conducted from July to November 1996 and May 1997 in Chiangmai Province, Thailand. Three villages urban, transition, rural areas were selected for the clarify spatial distribution Ae. aegypti albopictus along an urban–rural ecological gradient. The average number larvae larvitraps higher urban area than area, as we expected, whereas opposite found albopictus, > area. house larvae-inhabiting containers showed significant differences composition these...
Knowing the genetic variation that occurs in pathogen populations and how it is distributed across geographical areas essential to understand parasite epidemiology, local patterns of virulence, evolution host‐resistance. In addition, important identify pathogens are evolutionarily independent thus ‘free’ adapt hosts environments. Here, we investigated globally distributed, highly invasive avian malaria Plasmodium relictum , which has several distinctive mitochondrial haplotyps (cyt b...
IntroductionAedes albopictus (Skuse) is an important vector of arboviral diseases, including dengue, chikungunya and Zika virus disease. Monitoring insecticide resistance mechanisms by which the mosquito develops crucial to minimise disease transmission.AimTo determine status in Ae. from different geographical regions.MethodsWe sampled 33 populations Asia, Europe South America, tested these for susceptibility permethrin, a pyrethroid insecticide. In resistant populations, target site...
During the outbreak of highly pathogenic avian influenza that occurred in Tamba Town, Kyoto Prefecture 2004, a total 926 flies were collected from six sites within radius 2.3 km poultry farm. The H5 A virus genes detected intestinal organs, crop, and gut two blow fly species, Calliphora nigribarbis Aldrichina grahami, by reverse transcription-polymerase chain reaction for matrix protein (M) hemagglutinin (HA) genes. HA gene encoding multiple basic amino acids at cleavage site indicated this...
Bloodmeal identification and the detection of avian malaria parasite from mosquitoes (Diptera: Culicidae) were carried out by polymerase chain reaction-based methods for field samples collected in coastal areas Tokyo Bay, Japan, April to October 2007. The following seven mosquito species collected: Aedes albopictus (Skuse), Culex pipiens pallens Coquillett, form molestus Forskal, tritaeniorhynchus Giles, inatomii Kamimura & Wada, bitaeniorhynchus Lutzia vorax Edwards. Forty blood-fed 95%...
To evaluate the vectorial capacity of mosquitoes for viruses in Japan, host-feeding habits were analyzed by sequencing polymerase chain reaction-amplified fragments cytochrome b and 16S ribosomal RNA regions mitochondrial DNA 516 15 species from seven genera that collected residential areas during 2003-2006. Culex pipiens L. Aedes albopictus Skuse most commonly urban suburban areas. Anautogenous pallens Coquillett was distinguished autogenous Cx. form molestus Forskal using a reaction-based...
Heterogeneity in the transmission of mosquito-borne pathogens is determined largely by distribution patterns mosquito bites among wild animal populations. Although mosquitoes are crucial for avian malaria parasites, little known about ecology natural vectors. We examined bloodmeal and parasite incidence Culex pipiens pallens a polymerase chain reaction (PCR)-based procedure to determine how feeding pattern govern dynamics parasites Japanese birds. collected 881 unfed 486 blood-fed Cx....
Several species of captive and wild birds have been found to be infected with various avian blood protozoa in Japan. We investigated the prevalence transmission malaria parasite determined bloodmeal hosts mosquitoes collected a zoological garden Tokyo, Japan, by using polymerase chain reaction. In total, 310 unfed 140 blood-fed seven were sweep nets CDC traps. Bloodmeal identification indicated that had fed on 17 five mammalian species, including animals. The results detection from...
A total of 160 autochthonous dengue cases transmitted by Aedes albopictus were reported between August and October 2014 in Tokyo Metropolis, Japan. Ae. is a medically important vector virus, which has expanded its geographic distribution temperate regions. Understanding the biting density during outbreak to evaluate epidemic risks fever other highly populated cities Europe Asia. Of patients, 134 visited same park (Yoyogi Park) located central Tokyo. Mosquitoes infected with virus collected...
In 2014 in Japan, 162 autochthonous dengue cases were reported for the first time nearly 70 years. Here, we report results of detection and isolation virus (DENV) from mosquitoes collected Tokyo Metropolis 2015. The phylogenetic relationship among DENV isolates patients based on both entire envelope gene whole coding sequences was evaluated. Herein, 2,298 female 956 male Aedes albopictus at six suspected locations infection August to October grouped into 124 35 pools, respectively, viral...
The invasion of the yellow fever mosquito Aedes aegypti at Narita International Airport, Japan was detected for first time. During course routine vector surveillance 27 Ae. adults emerged from larvae and pupae collected a single larvitrap placed near No. 88 spot passenger terminal 2 on August 8, 2012. After appearance in larvitrap, we defined 400-m buffer zone started an intensive survey using additional 34 larvitraps 15 CO2 traps. aircraft were also inspected, one male cargo space...
Japanese encephalitis virus (JEV) is maintained in an enzootic cycle between swine, water birds, and mosquitoes. JEV has circulated indigenously Asia, with Culex tritaeniorhynchus as the primary vector. In some areas where vector scarce or absent, sporadic cases of have been reported, Aedes japonicus presumed to potential a secondary As one world’s most invasive culicid species, Ae . j carries considerable health risk for spreading diseases wider areas, including Europe North America. Thus,...
Several species of birds in Minami Daito Island, an oceanic island located the far south from main islands Japan, were found to be infected with avian Plasmodium. However, no vector malaria this have been revealed yet. To speculate potential vectors, we collected mosquitoes there and investigated using a PCR procedure whether harbor or not. Totally 1,264 including 9 during March 2006 February 2007. The stored every species, sampled date location for DNA extraction. Fifteen out 399 samples...
To evaluate the vectorial capacity of mosquitoes for viruses in Japan, host-feeding habits were analyzed by sequencing polymerase chain reaction-amplified fragments cytochrome b and 16S ribosomal RNA regions mitochondrial DNA 516 15 species from seven genera that collected residential areas during 2003–2006. Culex pipiens L. Aedes albopictus Skuse most commonly urban suburban areas. Anautogenous pallens Coquillett was distinguished autogenous Cx. form molestus Forskal using a reaction-based...
Internal transcribed spacer regions of ribosomal DNA were sequenced, and new species-specific primers designed to simplify the molecular identification five morphologically related subgenus Stegomyia mosquito species--Aedes aegypti, Ae. albopictus, riversi, flavopictus, daitensis--found in Ryukyu Archipelago, Japan. Each newly primer was able amplify a fragment with different size. Conditions for multiplex PCR optimized identify all species single PCR. This method is convenient tool...
Among members of the order Mononegavirales, RNA splicing events have been found only in family Bornaviridae. Here, we report that a new rhabdovirus isolated from mosquito Culex tritaeniorhynchus replicates nuclei infected cells and requires for viral mRNA maturation. The virus, designated (CTRV), shares similar genome organization with other rhabdoviruses, except presence putative intron coding region L protein. Molecular phylogenetic studies indicated CTRV belongs to Rhabdoviridae, but it...