A5088285492- Insect Resistance and Genetics
- Mosquito-borne diseases and control
- Insect Pest Control Strategies
- Insect and Pesticide Research
- Cholinesterase and Neurodegenerative Diseases
- Viral Infections and Vectors
- Dermatological diseases and infestations
- Malaria Research and Control
- Insects and Parasite Interactions
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Computational Drug Discovery Methods
- Bartonella species infections research
- Insect symbiosis and bacterial influences
- Insect-Plant Interactions and Control
- Insect and Arachnid Ecology and Behavior
- Pesticide Exposure and Toxicity
- Thin-Film Transistor Technologies
- Physiological and biochemical adaptations
- Semiconductor materials and devices
- Insect Utilization and Effects
- Viral Infectious Diseases and Gene Expression in Insects
- Study of Mite Species
- Bird parasitology and diseases
- CO2 Sequestration and Geologic Interactions
Sanno Medical Center
2025
Toshiba (Japan)
2025
Hitachi (United Kingdom)
2025
Mita Hospital
2023-2025
International University of Health and Welfare
2023-2025
National Institute of Infectious Diseases
2013-2023
Nagoya University
2023
Toyama Prefectural University
2020
Osaka University
1986-2018
National Institutes of Health
2000-2015
Aedes aegypti is the major vector of yellow and dengue fevers. After 10 generations adult selection, an A. strain (SP) developed 1650-fold resistance to permethrin, which one most widely used pyrethroid insecticides for mosquito control. SP larvae also 8790-fold following selection adults. Prior selections, frequencies V1016G F1534C mutations in domains II III, respectively, voltage-sensitive sodium channel (Vssc, target site insecticide) were 0.44 0.56, respectively. In contrast, only G1016...
The voltage-sensitive sodium (Na+) channel (Vssc) is the target site of pyrethroid insecticides. Pest insects develop resistance to this class insecticide by acquisition one or multiple amino acid substitution(s) in channel. In Southeast Asia, two major Vssc types confer dengue mosquito vector Aedes aegypti, namely, S989P+V1016G and F1534C. We expressed several Xenopus oocytes examined effect substitutions on susceptibilities. F1534C haplotypes reduced sensitivity permethrin 100- 25-fold,...
Background Resistance of Aedes aegypti to photostable pyrethroid insecticides is a major problem for disease-vector control programs. Pyrethroids target the voltage-gated sodium channel on insects' neurons. Single amino acid substitutions in this associated with resistance are one main factors that cause knockdown insects. Although kdr has been observed several mosquito species, point mutations para gene have not fully characterized Ae. populations Vietnam. The aim study was determine types...
The Asian tiger mosquito, Aedes albopictus (Skuse), is the major vector of Chikungunya fever and secondary dengue fever. We collected Ae. from Singapore performed genotyping assay to detect mutations voltage-gated sodium channel, which target site pyrethroid insecticides. detected an amino acid substitution, F1534C, suspected confer knockdown resistance (kdr) Of mosquitoes, 53.8% were homozygous for this mutation, allele frequency mutation was estimated be 73.1%. No kdr in 5 other loci...
Anopheles gambiae s.s., arabiensis, and funestus s.s. are the most important species for malaria transmission. Pyrethroid resistance of these vector mosquitoes is one main obstacles against effective control. The objective present study was to monitor pyrethroid susceptibility in 3 major vectors a highly endemic area western Kenya elucidate mechanisms species. Gembe East West, Mbita Division, 4 islands Suba district Nyanza province were used as area. Larval adult collection bioassay...
Abstract Recently-emerging genome editing technologies have enabled targeted gene knockout experiments even in non-model insect species. For studies on insecticide resistance, offer some advantages over the conventional reverse genetic technique, RNA interference, for testing causal relationships between genes of detoxifying enzymes and resistance phenotypes. There were relatively abundant evidences indicating that overexpression a cytochrome P450 CYP9M10 confers strong pyrethroid larvae...
IntroductionAedes albopictus (Skuse) is an important vector of arboviral diseases, including dengue, chikungunya and Zika virus disease. Monitoring insecticide resistance mechanisms by which the mosquito develops crucial to minimise disease transmission.AimTo determine status in Ae. from different geographical regions.MethodsWe sampled 33 populations Asia, Europe South America, tested these for susceptibility permethrin, a pyrethroid insecticide. In resistant populations, target site...
Background Knockdown resistance (kdr) to dichlorodiphenyltrichloroethane (DDT) and pyrethroids is known link amino acid substitutions in the voltage-gated sodium channel (VGSC) Aedes aegypti. Dengue fever primarily transmitted by Ae. aegypti an annual public health issue Taiwan. Accordingly, pyrethroid insecticides have been heavily used for decades control mosquito populations summer autumn. In Taiwan, population with two VGSC mutations, V1016G D1763Y, was described previously....
Aedes aegypti (Linnaeus, 1762) is the main mosquito vector for dengue and other arboviral infectious diseases. Control of this important highly relies on use insecticides, especially pyrethroids. The high frequency (>78%) L982W substitution was detected at target site pyrethroid insecticide, voltage-gated sodium channel (Vgsc) A. collected from Vietnam Cambodia. Alleles having concomitant mutations + F1534C V1016G were also confirmed in both countries, their (>90%) Phnom Penh, Strains these...
A microsomal cytochrome P450 from the house fly (Musca domestica), termed P450lpr, is involved in monooxygenase-mediated pyrethroid resistance and expressed at 8-fold higher levels insecticide resistant LPR strain compared to a susceptible strain. An internal cDNA sequence was amplified by polymerase chain reaction (PCR) using degenerate primers based on known polypeptide sequences, remainder of single side-specific PCR. 1.8 kb obtained 3 overlapping PCR products, with an open reading frame...
Trench fever is a body louse-borne disease caused by Bartonella quintana Brenner. The recent status of louse infestation in Nepalese children not well known. We collected head and lice, Pediculus humanus capitis De Geer L., respectively, from 30 children, including 11 cases double with both lice. Detection B. species identified was carried out polymerase chain reaction (PCR). PCR products DNA sequences were detected lice two as derived other children. These results demonstrate that may also...
ABSTRACT The symbiotic bacteria associated with the stomach disc, a large aggregate of bacteriocytes on ventral side midgut, human body and head lice were characterized. Molecular phylogenetic analysis 16S rRNA gene sequences showed that symbionts formed distinct well-defined clade in Gammaproteobacteria . exhibited AT-biased nucleotide composition accelerated molecular evolution. In situ hybridization revealed nymphs adult males, symbiont was localized while females, not disc but lateral...
A total of 160 autochthonous dengue cases transmitted by Aedes albopictus were reported between August and October 2014 in Tokyo Metropolis, Japan. Ae. is a medically important vector virus, which has expanded its geographic distribution temperate regions. Understanding the biting density during outbreak to evaluate epidemic risks fever other highly populated cities Europe Asia. Of patients, 134 visited same park (Yoyogi Park) located central Tokyo. Mosquitoes infected with virus collected...
Long-term exposure to anticancer agents poses a health risk healthcare workers and requires safety measures such as biological cabinets personal protective equipment. The purpose of this study was verify the usefulness two drug-degrading agents, HD Protect (Secom Medical System Co. Ltd., Tokyo, Japan) Tripleclin (Nipro Settsu, Japan), by analyzing residual amounts fluorouracil (5-FU), which is frequently used in real clinical practice. Wiping with showed lower 5-FU concentrations than wiping...
Abstract The complete coding sequences of two acetylcholinesterase (AChE) genes, Ace1 (orthologous to Drosophila Ace ) and Ace2 (paralogous ), from the cotton aphid ( Aphis gossypii were identified carbamate resistant susceptible strains compared. No change in amino acid was found , while substitutions, Ser431Phe Ala302Ser, detected between . position corresponds one fourteen aromatic residues lining active site gorge is located acyl pocket. Ala302Ser at three which form oxyanion hole AChE....