A5027630529- Mosquito-borne diseases and control
- Insect Resistance and Genetics
- Insect Pest Control Strategies
- Viral Infections and Vectors
- Malaria Research and Control
- Insect and Pesticide Research
- Vector-Borne Animal Diseases
- Insect symbiosis and bacterial influences
- Insect-Plant Interactions and Control
- Vector-borne infectious diseases
- Dermatological diseases and infestations
- Zoonotic diseases and public health
- Parasites and Host Interactions
- Cholinesterase and Neurodegenerative Diseases
- Insects and Parasite Interactions
- Glutathione Transferases and Polymorphisms
- Animal Ecology and Behavior Studies
- Parasite Biology and Host Interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Dengue and Mosquito Control Research
- Entomopathogenic Microorganisms in Pest Control
- Venomous Animal Envenomation and Studies
- Fern and Epiphyte Biology
- Beetle Biology and Toxicology Studies
- Bird parasitology and diseases
National Institute of Infectious Diseases
2015-2025
National Institutes of Health
2006-2015
Sumitomo Chemical (Japan)
2009
Pakistan Agricultural Research Council
1996
Chiba University
1996
Aedes aegypti is the major vector of yellow and dengue fevers. After 10 generations adult selection, an A. strain (SP) developed 1650-fold resistance to permethrin, which one most widely used pyrethroid insecticides for mosquito control. SP larvae also 8790-fold following selection adults. Prior selections, frequencies V1016G F1534C mutations in domains II III, respectively, voltage-sensitive sodium channel (Vssc, target site insecticide) were 0.44 0.56, respectively. In contrast, only G1016...
The voltage-sensitive sodium (Na+) channel (Vssc) is the target site of pyrethroid insecticides. Pest insects develop resistance to this class insecticide by acquisition one or multiple amino acid substitution(s) in channel. In Southeast Asia, two major Vssc types confer dengue mosquito vector Aedes aegypti, namely, S989P+V1016G and F1534C. We expressed several Xenopus oocytes examined effect substitutions on susceptibilities. F1534C haplotypes reduced sensitivity permethrin 100- 25-fold,...
Background Resistance of Aedes aegypti to photostable pyrethroid insecticides is a major problem for disease-vector control programs. Pyrethroids target the voltage-gated sodium channel on insects' neurons. Single amino acid substitutions in this associated with resistance are one main factors that cause knockdown insects. Although kdr has been observed several mosquito species, point mutations para gene have not fully characterized Ae. populations Vietnam. The aim study was determine types...
The Asian tiger mosquito, Aedes albopictus (Skuse), is the major vector of Chikungunya fever and secondary dengue fever. We collected Ae. from Singapore performed genotyping assay to detect mutations voltage-gated sodium channel, which target site pyrethroid insecticides. detected an amino acid substitution, F1534C, suspected confer knockdown resistance (kdr) Of mosquitoes, 53.8% were homozygous for this mutation, allele frequency mutation was estimated be 73.1%. No kdr in 5 other loci...
The Aedes mosquito, which transmits the dengue fever virus and other viruses, has acquired resistance to pyrethroid insecticides in a naturally selective manner. Massive use of led worldwide expansion resistant populations. major factor is knockdown (kdr) caused by amino acid mutation(s) voltage-gated sodium channel, target site this insecticide group. Some kdr mutations can lead dramatic increase resistance, multiple level 10 several-hundred. In review, we summarize identified mosquitoes...
Anopheles gambiae s.s., arabiensis, and funestus s.s. are the most important species for malaria transmission. Pyrethroid resistance of these vector mosquitoes is one main obstacles against effective control. The objective present study was to monitor pyrethroid susceptibility in 3 major vectors a highly endemic area western Kenya elucidate mechanisms species. Gembe East West, Mbita Division, 4 islands Suba district Nyanza province were used as area. Larval adult collection bioassay...
Abstract Recently-emerging genome editing technologies have enabled targeted gene knockout experiments even in non-model insect species. For studies on insecticide resistance, offer some advantages over the conventional reverse genetic technique, RNA interference, for testing causal relationships between genes of detoxifying enzymes and resistance phenotypes. There were relatively abundant evidences indicating that overexpression a cytochrome P450 CYP9M10 confers strong pyrethroid larvae...
IntroductionAedes albopictus (Skuse) is an important vector of arboviral diseases, including dengue, chikungunya and Zika virus disease. Monitoring insecticide resistance mechanisms by which the mosquito develops crucial to minimise disease transmission.AimTo determine status in Ae. from different geographical regions.MethodsWe sampled 33 populations Asia, Europe South America, tested these for susceptibility permethrin, a pyrethroid insecticide. In resistant populations, target site...
Aedes aegypti (Linnaeus, 1762) is the main mosquito vector for dengue and other arboviral infectious diseases. Control of this important highly relies on use insecticides, especially pyrethroids. The high frequency (>78%) L982W substitution was detected at target site pyrethroid insecticide, voltage-gated sodium channel (Vgsc) A. collected from Vietnam Cambodia. Alleles having concomitant mutations + F1534C V1016G were also confirmed in both countries, their (>90%) Phnom Penh, Strains these...
A total of 160 autochthonous dengue cases transmitted by Aedes albopictus were reported between August and October 2014 in Tokyo Metropolis, Japan. Ae. is a medically important vector virus, which has expanded its geographic distribution temperate regions. Understanding the biting density during outbreak to evaluate epidemic risks fever other highly populated cities Europe Asia. Of patients, 134 visited same park (Yoyogi Park) located central Tokyo. Mosquitoes infected with virus collected...
Background Aedes aegypti transmits various arthropod-borne diseases such as dengue, posing a significant burden to public health in tropical and subtropical regions. Pyrethroid-based control strategies are effective managing this vector; however, the development of insecticide resistance has hindered these efforts. Hence, long-term monitoring mosquito populations is crucial for vector disease control. Methodology/principal findings In study, we identified due voltage-gated sodium channel (...
Human schistosomiasis is a serious parasitic disease caused by infection with Schistosoma japonicum. In order to establish monitoring sites in the S. japonicum endemic area Obitsu River basin Chiba Prefecture, Japan, we investigated habitat of intermediate snail host, Oncomelania hupensis nosophora using geographic information systems (GIS). A series thematic maps soil types, land-use, and past wetlands were compared distributions patients O. h. habitats identify environmental conditions...
In Kenya, insecticide-treated mosquito nets (ITNs) distributed to pregnant women and children under 5 years old through various programs have resulted in a significant reduction malaria deaths. All of the World Health Organization-recommended insecticides for are pyrethroids, vector resistance these is one major obstacles an effective control program. Anopheles gambiae s.s. arabiensis vectors that widely Kenya. Two point mutations voltage-gated sodium channel (L1014F L1014S) associated with...
Abstract Previous studies on a strain of Culex pipiens quinquefasciatus from Saudi Arabia indicated permethrin resistance was result cytochrome P450 mediated detoxification and kdr . The found to be larval specific associated with fitness cost in certain environments. responsible for (and the cost) has not been identified, but recently two candidate P450s ( CYP4H34 CYP9M10 ) have found. We measured b 5 content as well expression levels susceptible (SLAB) isogenic strains (isolated by...
Pyrethroid insecticides play very important roles in the control of pediculosis, which is caused by human head louse (Pediculus humanus capitis De Geer) worldwide. The development resistance to pyrethroid pediculicides has become a global issue during last two decades. associated with amino acid substitutions α-subunit para-sodium channel gene; these are termed kdr-like mutations. frequencies four mutations (D11E, M815I, T929I, and L932F) sodium gene were studied colonies collected from...
The Culex pipiens complex consists of vector mosquitoes that transmit important human pathogens. In this study we established a simplified method to distinguish three members the Cx. complex, p. pallens Coquillet, form molestus Forskal, and quinquefasciatus Say, collected in Japan. Sequence analysis Drosophila Ace-orthologous acetylcholinesterase (Ace) gene (668 680 bp) revealed single polymorphic region characterizes each species. Based on region, specific primers (ACEpip2) (ACEpall2) were...
Abstract BACKGROUND Aedes aegypti is a remarkably effective mosquito vector of epidemiologically important arboviral diseases including dengue fever, yellow fever and Zika. The present spread resistance against pyrethroids, the primary insecticides used for control, in global populations this species great concern. voltage‐gated sodium channel (VGSC) nervous system known target site pyrethroids insects. Past studies have revealed several amino‐acid substitutions that confer pyrethroid...
Although body louse is a well-known vector of trench fever, the growth kinetics Bartonella quintana in lice has not been fully understood. We performed quantitative analysis bacterial multiplication rate. B. started proliferation 4 days after ingestion and was constantly excreted feces for at least 3 weeks. The number bacteria reached maximum 10(7)/louse per day on Day 15. doubling time estimated from logistic regression formula 21.3 hours. Scanning electron microscopy showed presence masses...