A5000132523- Gut microbiota and health
- Probiotics and Fermented Foods
- Clostridium difficile and Clostridium perfringens research
- Phytoestrogen effects and research
- Digestive system and related health
- Food composition and properties
- Escherichia coli research studies
- Immune Cell Function and Interaction
- Animal Nutrition and Physiology
- Viral Infections and Vectors
- Diet and metabolism studies
- X-ray Diffraction in Crystallography
- Infant Nutrition and Health
- Microbial Metabolites in Food Biotechnology
- Mosquito-borne diseases and control
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- Crystallization and Solubility Studies
- Leptospirosis research and findings
- Gastrointestinal motility and disorders
- Diet, Metabolism, and Disease
- Dengue and Mosquito Control Research
- Enterobacteriaceae and Cronobacter Research
- Polysaccharides and Plant Cell Walls
- Heavy Metal Exposure and Toxicity
The University of Tokyo
2012-2024
Nagasaki University
2022-2024
Kagoshima University
2022
University of Tsukuba
2016
Karolinska Institutet
1999-2003
Tokyo University of Agriculture
1989-1999
It has been demonstrated that intestinal commensal bacteria induce immunoglobulin (Ig) A production by promoting the development of gut-associated lymphoid tissues in small intestine. However, precise mechanism whereby these modulate IgA large intestine, which harbors majority commensals, is poorly understood. In addition, it not known intestine and To address issues, we generated gnotobiotic mice mono-associated with different murine inoculating germ-free (GF) Lactobacillus johnsonii or...
Japanese encephalitis (JE) remains a public health concern in several countries, and the Culex mosquito plays central role its transmission cycle. mosquitoes harbor wide range of viruses, including insect-specific viruses (ISVs), can transmit variety arthropod-borne (arboviruses) that cause human animal diseases. The current trend studies displays enhanced efforts to characterize virome through bulk RNA sequencing due possible arbovirus–ISV interactions; however, extent viral diversity taxon...
Histochemical, lectin-histochemical and morphometrical studies were carried out on intestinal goblet cells of 8-week-old germfree (GF) conventional (CV) mice the BALB/c strain. Except for reactivity cecal to Dolichos biflorus agglutinin (DBA) Ulex europeus-I (UEA-I), there was no difference between GF CV in histochemical properties. In mucosa, DBA stained strongly but not UEA-I lower part crypts only faintly mice. These findings suggest that terminal residues cell mucin different...
Although it is established that the composition of human intestinal microbiota changes with age, transition animals age has not been well studied. In present study, we collected fresh fecal samples from dogs 5 different groups (pre-weanling, weanling, young, aged, senile) and analyzed compositions their a culture-based method. The results suggested canine also age. Among bacteria predominant in dog intestines, lactobacilli appeared to change Both number prevalence tended decrease when became...
Objective Although immunoglobulin A (IgA) is abundantly expressed in the gut and known to be an important component of mucosal barriers against luminal pathogens, its precise function remains unclear. Therefore, we tried elucidate effect IgA on homeostasis maintenance mechanism. Design We generated various mutant mouse lines using CRISPR/Cas9 genome editing system. Then, evaluated small intestinal homeostasis, pathology, microbiota, cytokine production, immune cell activation intravital...
Japanese encephalitis virus (JEV) is maintained in an enzootic cycle between swine, water birds, and mosquitoes. JEV has circulated indigenously Asia, with Culex tritaeniorhynchus as the primary vector. In some areas where vector scarce or absent, sporadic cases of have been reported, Aedes japonicus presumed to potential a secondary As one world’s most invasive culicid species, Ae . j carries considerable health risk for spreading diseases wider areas, including Europe North America. Thus,...
Abstract Recent research suggests that protein deficiency symptoms are influenced by the intestinal microbiota. We investigated influence of low diet on composition microbiota through animal experiments. Specific pathogen-free (SPF) mice were fed one four diets (3, 6, 9, or 12% protein) for 4 weeks (n = 5 per diet). Mice 3% showed such as weight loss and level blood urea nitrogen concentration in their serum. The groups at day 0, 7, 14, 21 28 16S rRNA gene sequencing, which revealed...
Endothelin receptor null rats [ETB(-/-)] are a model for long-segment Hirschsprung's disease. These animals have significant intestinal distension (megaileum) proximal to constricted region of the gastrointestinal tract lacking enteric ganglia. Experiments were performed determine pathophysiological changes that occur in these and examine tunica muscularis as unique, immunologically active compartment. We observed abnormal flora ETB(-/-) rats, which included marked increase gram-negative...
The gut mucosal immune system is crucial in host defense against infection by pathogenic microbacteria and viruses via the production of IgA. Previous studies have shown that intestinal commensal bacteria enhance IgA production. However, it poorly understood how these induce which genera effectively. In this study, we compared immunomodulatory effects Bacteroides Lactobacillus on Peyer's patches lymphocytes. lymphocytes co-cultured with was higher than Lactobacillus. addition, expression...
Abstract Gut microbiota plays an important role in regulating brain function and adult neurogenesis. Although probiotics have recently been reported as effective against certain psychiatric disorders, the underlying mechanisms remain unclear. In particular, combination of 3 probiotic strains, Bacillus subtilis TO-A, Enterococcus faecium T-110, Clostridium butyricum hereafter referred to ProB3, has potentially alleviate symptoms patients with schizophrenia. Herein, we show that ProB3 promotes...
ABSTRACT: Few reports have considered the effects of dietary fiber on plasma quercetin and intestinal flora. We investigated pectin fecal flora mice fed a diet supplemented with glycoside rutin. Male were randomly divided into 2 groups, which pectin–rutin (PR) or cellulose–rutin (CR) for 14 d. Plasma isorhamnetin metabolites measured by high‐performance liquid chromatography. Feces immediately processed bacteriological procedures. The was investigated. concentrations significantly higher in...
The transition of intestinal microbiota with age has been well described in humans. However, the age-related changes cats have not studied. In present study, we investigated composition 5 different groups (pre-weanling, weanling, young, aged, senile) a culture-based method. For lactobacilli and bifidobacteria, also quantified molecular-based method, real-time PCR. results suggested that feline age, while were from those humans dogs. Bifidobacteria which are predominant human intestine or dog...
Japanese encephalitis virus (JEV) is transmitted between swine, migratory birds, and Culex mosquitoes, has circulated indigenously in Asia for almost a century. Despite being the country with highest JEV diversity, surveillance targeting of Indonesia's vectors scarce. This study collected mosquitoes from several locations Tabanan Regency, Bali Island, Indonesia. We captured classified 3,032 adult into seven species, vishnui subgroup making up approximately 90% total. was identified by...
The composition of faecal microbiota, enzyme activities bacteria and concentrations putrefactive products short chain fatty acids (SCFAs) were studied in 'human-flora-associated' (HFA) mice inoculated with six different human faeces. major HFA was similar to that faeces, although bifidobacteria eliminated from some mouse groups. β-Glucuronidase activity intestinal humans. However nitroreductase closer conventional than Although the SCFAs much lower those humans, Individual variations...
Probiotics, such as lactic acid bacteria (LAB) and Bacillus subtilis var. natto, have been shown to modulate immune responses. It is important understand how probiotic impact intestinal epithelial cells (IECs), because IECs are the first line of defense at mucosal surface barrier their activities substantially affect gut microenvironment immunity. However, date, precise mechanism remains unknown due a lack analytical systems available for live animal models. Recently, we generated...
Antimicrobial resistance (AMR) is a global public health concern for both clinical and veterinary medicine. Rodent feces are one of the major infectious sources zoonotic pathogens including AMR bacteria. So far, there limited studies reported focused on Escherichia coli isolated in rodent from rural suburban areas Vietnam. In this study, we investigated prevalence antimicrobial E. samples 144 urban rodents caught Hanoi, A total 59 was which 42 were multidrug-resistant (MDR) isolates...
Nasal congestion in allergic rhinitis (AR) is caused by vascular hyperpermeability and relaxation of the nasal mucosa. We previously detected high levels a lipoxygenation metabolite dihomogammalinolenic acid, 15-hydroxy-8Z,11Z,13E-eicosatrienoic acid (15-HETrE) lavage fluid AR model mice. Here, we investigated effects 15-HETrE on functions associated with congestion. measured ovalbumin-induced mice discharge patients AR. also assessed Vascular contractility was using isolated mouse aortas....
Viral gastrointestinal infections are an important public health concern, and the occurrence of asymptomatic enteric virus makes it difficult to prevent control their spread. This study aimed determine prevalence factors associated with infection in adults northern Laos. Fecal samples were collected from apparently healthy participants who did not report diarrhea or high fever at time survey Laos, viruses detected using polymerase chain reaction (PCR) reverse transcription (RT)-PCR....
Although the intestinal flora is believed to have a critical role in carcinogenesis, little known about of human on effects mutagens vivo. The aim present study was address possible by exploiting human-flora-associated (HFA) mice. capacity faeces activate or inactivate 2-amino-3-methyl-3H-imidazo[4,5-f]quinoline (IQ) and 2-nitrofluorene determined using Ames assay. Human faecal suspensions that were active this regard then selected orally inoculated into germfree NMRI mice generate HFA HFA,...