Journal Article Prostaglandin F2α Synthase Activities of Aldo–Keto Reductase 1B1, 1B3 and 1B7 Get access Zakayi Kabututu, Kabututu 1Department Molecular Behavioral Biology, Osaka Bioscience Institute, 6-2-4 Furuedai, Suita, 565-0874, Japan; 2CNRS, UMR6247—Genetic, Reproduction Development (GReD), Clermont University, 63177 Aubière, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Michèle Manin, Manin Jean-Christophe Pointud, Pointud Toshihiko Maruyama,...

Upon immediate allergic inflammation, activated mast cells produce abundant bioactive lipid mediator prostaglandin (PG) D2. PGD2 is metabolised and excreted in urine as tetranor-PGDM. We have previously reported urinary tetranor-PGDM a sensitive biomarker for food allergy (FA) reactions children [1]. However, the production profile of other mediators FA patients remains unknown. Bioactive lipids are produced by enzyme-dependent or independent metabolism polyunsaturated fatty acids (PUFAs)...

Prostaglandin (PG) F(2alpha) suppresses adipocyte differentiation by inhibiting the function of peroxisome proliferator-activated receptor gamma. However, PGF(2alpha) synthase (PGFS) in adipocytes remains to be identified. Here, we studied expression members aldo-keto reductase (AKR) 1B family acting as PGFS during adipogenesis mouse 3T3-L1 cells. AKR1B3 mRNA was expressed preadipocytes, and its level increased about 4-fold at day 1 after initiation differentiation, then quickly decreased...

Urinary excretion of lipocalin-type PGD(2) synthase (L-PGDS), which converts PG H(2) to PGD(2), increases in early diabetic nephropathy. In addition, L-PGDS expression the tubular epithelium adriamycin-induced nephropathy, suggesting that locally produced may promote development CKD. this study, we found L-PGDS-derived contributes progression renal fibrosis via CRTH2-mediated activation Th2 lymphocytes. a mouse model, synthesized de novo after unilateral ureteral obstruction (UUO)....

1. Protease-activated receptors (PARs) 1 and 2 modulate the gastric intestinal smooth muscle motility in vitro. In present study, we examined if activation of PAR-2 PAR-1 could alter gastrointestinal transit mice. 2. Intraperitoneal administration PAR-2-activating peptide SLIGRL-NH(2), but not inactive control LSIGRL-NH(2), at - 5 micromol kg(-1), combination with aminopeptidase inhibitor amastatin 2.5 facilitated a dose-dependent manner. The human PAR-1-derived SFLLR-NH(2) specific agonist...

We report here that 4-dibenzo[a,d]cyclohepten-5-ylidene-1-[4-(2H-tetrazol-5-yl)-butyl]-piperidine (AT-56) is an orally active and selective inhibitor of lipocalin-type prostaglandin (PG) D synthase (L-PGDS). AT-56 inhibited human mouse L-PGDSs in a concentration (3-250 microm)-dependent manner but did not affect the activities hematopoietic PGD (H-PGDS), cyclooxygenase-1 -2, microsomal PGE synthase-1. L-PGDS activity competitive against substrate PGH(2) (K(m) = 14 microm) with K(i) value 75...

Idiopathic normal pressure hydrocephalus in elderly people is considered a form of glymphopathy caused by malfunction the waste clearance pathway, called glymphatic system. Tau representative material similar to amyloid-β. During neurodegeneration, lipocalin-type prostaglandin D synthase (L-PGDS), major cerebrospinal fluid (CSF) protein, reported act as chaperone that prevents neurotoxic aggregation L-PGDS also CSF biomarker idiopathic and significantly correlates with tau concentration,...

Scope We found that rubiscolin‐6, a δ opioid agonist peptide derived from d ‐ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubisco), major protein of green leaves, stimulates food intake after oral administration in mice. therefore investigated its mechanism. Methods and results Orexigenic activity rubiscolin‐6 was blocked by central naltrindole, an antagonist for receptor, suggesting orally administered via receptor activation. The orexigenic inhibited celecoxib, cyclooxygenase ( COX )‐2...

Lipocalin-type prostaglandin D synthase (L-PGDS) is a multifunctional protein that produces D(2) and binds transports various lipophilic substances after secretion into body fluids as beta-trace. L-PGDS has been proposed to be useful diagnostic marker for renal injury associated with diabetes or hypertension, because the urinary plasma concentrations are increased in patients these diseases. However, it remains unclear whether synthesized de novo kidney taken up from blood circulation. In...

Aldo-keto reductase 1B1 and 1B3 (AKR1B1 AKR1B3) are the primary human mouse prostaglandin F(2α) (PGF(2α)) synthases, respectively, which catalyze NADPH-dependent reduction of PGH(2), a common intermediate various prostanoids, to form PGF(2α). In this study, we found that AKR1B1 AKR1B3, but not AKR1B7 AKR1C3, also catalyzed isomerization PGH(2) PGD(2) in absence NADPH or NADP(+). Both PGF(2α) synthase activities AKR1B3 completely disappeared presence NADP(+) after heat treatment these enzymes...

Nasal congestion in allergic rhinitis (AR) is caused by vascular hyperpermeability and relaxation of the nasal mucosa. We previously detected high levels a lipoxygenation metabolite dihomogammalinolenic acid, 15-hydroxy-8Z,11Z,13E-eicosatrienoic acid (15-HETrE) lavage fluid AR model mice. Here, we investigated effects 15-HETrE on functions associated with congestion. measured ovalbumin-induced mice discharge patients AR. also assessed Vascular contractility was using isolated mouse aortas....

The pathogenesis of ulcerative colitis (UC) is unclear, but enhancement disease activity by usage nonsteroidal anti-inflammatory drugs suggests involvement prostanoid in its pathophysiology. However, biological effect prostaglandin (PG) D(2) on intestinal inflammation remains unknown. We investigated the expression enzymes for PGD(2) synthesis, D synthase (PGDS), and relation to UC patients. role lipocalin-type PGDS (L-PGDS) using a murine model was also assessed. Tissue samples were...

Atopic dermatitis (AD) is the most common inflammatory skin disease in children. The serum level of thymus and activation-regulated chemokine (TARC) a useful AD index to reflect severity; however, it requires blood collection from young In comparison, urine samples are easier collect pediatric clinical setting. Here, we analyzed lipids excreted identify diagnostic biomarker for AD. We generated murine model by repeated topical application 2,4-dinitrofluorobenzene (DNFB) or tape-stripping...

Zinc is an essential trace element for humans and animals, being located, among other places, in the synaptic vesicles of cortical glutamatergic neurons hippocampal mossy fibers brain. Extracellular zinc has potential to interact with modulate many different targets, including glutamate GABA receptors. Because central role these neurotransmitters brain activity, we examined this study sleep-promoting activity by monitoring locomotor electroencephalogram after its administration mice....

Idiopathic normal pressure hydrocephalus (iNPH) is a treatable cause of dementia, gait disturbance, and urinary incontinence in elderly patients with ventriculomegaly. Its unique morphological feature, called disproportionately enlarged subarachnoid-space (DESH), may also be diagnostic feature. Lipocalin-type prostaglandin D synthase (L-PGDS) major cerebrospinal fluid (CSF) protein produced by arachnoid cells, its concentration the CSF reportedly decreased iNPH. L-PGDS acts as D2-producing...

Abstract Lipocalin-type prostaglandin (PG) D synthase (L-PGDS) is responsible for the production of PGD 2 in adipocytes and selectively induced by a high-fat diet (HFD) adipose tissue. In this study, we investigated effects HFD on obesity insulin resistance two distinct types adipose-specific L-PGDS gene knockout (KO) mice: fatty acid binding protein 4 ( fabp4 , aP2 ) -Cre/L-PGDS flox/flox adiponectin AdipoQ mice. The was deleted premature stage former strain after maturation latter strain....

Rapid eye movement (REM) sleep loss is associated with increased consumption of weight-promoting foods. The prefrontal cortex (PFC) thought to mediate reward anticipation. However, the precise role PFC in mediating responses highly palatable foods (HPF) after REM deprivation unclear. We selectively reduced mice over a 25–48 hr period and chemogenetically inhibited medial (mPFC) by using an altered glutamate-gated ivermectin-gated chloride channel that facilitated neuronal inhibition through...

Abstract Acute lung injury (ALI) is caused by various stimuli such as acid aspiration and infection, resulting in severe clinical outcomes with high mortality. Prostaglandin D 2 (PGD ) a lipid mediator produced the lungs of patients ALI. There are two prostaglandin synthases (PGDS), namely, lipocalin‐type PGDS (L‐PGDS) hematopoietic (H‐PGDS). We previously reported anti‐inflammatory role H‐PGDS‐derived PGD an endotoxin‐induced murine ALI model. Therefore, this study, we investigated...

Although prostaglandins (PGs) are known to be involved in the progression of arthritis, role PGD2 remains unclear. In this study, we evaluated joint inflammation using genetically modified mice. Injection complete Freund's adjuvant (CFA) increased production and induced paw swelling cartilage erosion wild-type (WT) These phenomena were accompanied with an increase mRNA levels TNF-α, IL-6, IL-1β, matrix-degrading metalloproteinase-9. Knockdown hematopoietic PGD synthase (H-PGDS) abolished...

5,6-dihydroxy-8Z,11Z,14Z,17Z-eicosatetraenoic acid (5,6-DiHETE) is an eicosapentaenoic acid-derived lipid metabolite, which we previously detected in inflamed mouse colon. In this study, investigated the pathophysiological roles of 5,6-DiHETE murine colitis and its underlying mechanisms action, focusing on effects transient receptor potential vanilloid (TRPV) channel activity. Oral administration dextran sodium sulfate (DSS, 2%, for 4 days) caused colon inflammation, peaked day 7 gradually...

Immunological responses in the host can result different disease outcomes of Helicobacter pylori-induced gastritis. Prostaglandin E2 derived from cyclooxygenase (COX) and prostaglandin E synthase contribute to gastric protection. Recently, D2 was shown be involved immunity by chemotactic activity through chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2), but its role H. gastritis has not been clarified. We determined expression levels mRNAs for haematopoietic PGD...

In diabetic retinopathy (DR), pericyte dropout from capillary walls is believed to cause the breakdown of blood-retina barrier (BRB), which subsequently leads vision-threatening retinal edema. While various proinflammatory cytokines and chemokines are upregulated in eyes with DR, their distinct contributions disease progression remain elusive. Here, we evaluated roles stromal cell–derived factor-1α (SDF-1α) its receptor CXCR4 BRB initiated by deficiency. After inhibition recruitment...