A5025091127- Sleep and Wakefulness Research
- Neuroscience and Neuropharmacology Research
- Circadian rhythm and melatonin
- Endoplasmic Reticulum Stress and Disease
- Sleep and related disorders
- RNA regulation and disease
- Neuroendocrine regulation and behavior
- Memory and Neural Mechanisms
- Neuroscience of respiration and sleep
- Biochemical Analysis and Sensing Techniques
- Neurotransmitter Receptor Influence on Behavior
- Stress Responses and Cortisol
- Adipose Tissue and Metabolism
- RNA Research and Splicing
- Neurogenesis and neuroplasticity mechanisms
- Adenosine and Purinergic Signaling
- Olfactory and Sensory Function Studies
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- CRISPR and Genetic Engineering
- Tryptophan and brain disorders
- Genetics and Neurodevelopmental Disorders
- Regulation of Appetite and Obesity
- RNA and protein synthesis mechanisms
Tsukuba International University
2016-2025
University of Tsukuba
2016-2025
StemCells (United States)
2018
Institut National de la Recherche Agronomique
2005-2010
Osaka University
2010
Unité de Nutrition Humaine
2007-2009
Centre de Recherche en Nutrition Humaine d'Auvergne
2004-2009
Osaka Science Museum
2009
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2007
Génétique Médicale & Génomique Fonctionelle
2004
Caffeine, the most widely used psychoactive compound, is an adenosine receptor antagonist. It promotes wakefulness by blocking A<sub>2A</sub> receptors (A<sub>2A</sub>Rs) in brain, but specific neurons on which caffeine acts to produce arousal have not been identified. Using selective gene deletion strategies based Cre/loxP technology mice and focal RNA interference silence expression of A<sub>2A</sub>Rs rats local infection with adeno-associated virus carrying short-hairpin RNA, we report...
Nucleus accumbens (NAc) is involved in behaviors that depend on heightened wakefulness, but its impact arousal remains unclear. Here, we demonstrate NAc dopamine D
Abstract Sleep control is ascribed to a two-process model, widely accepted concept that posits homoeostatic drive and circadian process as the major sleep-regulating factors. Cognitive emotional factors also influence sleep–wake behaviour; however, precise circuit mechanisms underlying their effects on sleep are unknown. Previous studies suggest adenosine has role affecting behavioural arousal in nucleus accumbens (NAc), brain area critical for reinforcement reward. Here, we show...
The sleep cycle is characterized by alternating non-rapid eye movement (NREM) and rapid (REM) sleeps. mechanisms which this generated are incompletely understood. We found that a transient increase of dopamine (DA) in the basolateral amygdala (BLA) during NREM terminates initiates REM sleep. DA acts on receptor D2 (Drd2)-expressing neurons BLA to induce NREM-to-REM transition. This mechanism also plays role cataplectic attacks-a pathological intrusion into wakefulness-in narcoleptics. These...
The integrated stress response (ISR) is defined as a highly conserved to several stresses that converge the induction of activating transcription factor 4 (ATF4). Because an uncontrolled may have deleterious effects, cells elaborated negative feedback loops attenuate ISR. In present study, we describe how human homolog Drosophila tribbles (TRB3) attenuates ISR by mechanism. To investigate role TRB3 in control ISR, used regulation gene expression amino acid limitation model. enhanced...
Dysfunction of the striatum is frequently associated with sleep disturbances. However, its role in sleep-wake regulation has been paid little attention even though densely expresses adenosine A2A receptors (A2ARs), which are essential for adenosine-induced sleep. Here we showed that chemogenetic activation A2AR neurons specific subregions induced a remarkable increase non-rapid eye movement (NREM) Anatomical mapping and immunoelectron microscopy revealed striatal innervated external globus...
Abstract The ventral pallidum (VP) regulates motivation, drug addiction, and several behaviors that rely on heightened arousal. However, the role underlying neural circuits of VP in control wakefulness remain poorly understood. In present study, we sought to elucidate specific GABAergic neurons controlling sleep–wake mice. Fiber photometry revealed population activity was increased during physiological transitions from non-rapid eye movement (non-REM, NREM) sleep either or REM sleep....
Evidence has accumulated that amino acids play an important role in controlling gene expression. Nevertheless, two components of the acid control expression are not yet completely understood mammals: (a) target genes and biological processes regulated by availability, (b) signaling pathways mediate response. Using large-scale analysis expression, objective this study was to gain a better understanding limitation. We found 6 h period leucine starvation specific set genes: 420 were...
Innate behaviors are genetically encoded, but their underlying molecular mechanisms remain largely unknown. Predator odor 2,4,5-trimethyl-3-thiazoline (TMT) and its potent analog 2-methyl-2-thiazoline (2MT) believed to activate specific odorant receptors elicit innate fear/defensive in naive mice. Here, we conduct a large-scale recessive genetics screen of ethylnitrosourea (ENU)-mutagenized We find that loss Trpa1, pungency/irritancy receptor, diminishes TMT/2MT snake skin-evoked responses....
The rostromedial tegmental nucleus (RMTg), also called the GABAergic tail of ventral area, projects to midbrain dopaminergic system, dorsal raphe nucleus, locus coeruleus, and other regions. Whether RMTg is involved in sleep–wake regulation unknown. In present study, pharmacogenetic activation rat neurons promoted non-rapid eye movement (NREM) sleep with increased slow-wave activity (SWA). Conversely, rats after neurotoxic lesions 8 or 16 days showed decreased NREM reduced SWA at lights on....
The supramammillary nucleus (SuM) provides substantial innervation to the dentate gyrus (DG). It remains unknown how SuM and DG coordinate their activities at circuit level regulate spatial memory. Additionally, co-releases GABA glutamate DG, but relative role of versus in regulating memory unknown. Here we report that SuM-DG Ca 2+ are highly correlated during retrieval as compared moderate correlation encoding when mice performing a location discrimination task. Supporting this evidence,...
Activation of the parabrachial nucleus (PB) in brainstem induced wakefulness rats, suggesting which is an important that controls arousal. However, sub-regions PB regulating sleep-wake cycle still unclear. Here, we employ chemogenetics and optogenetics strategies find activation medial part (MPB), but not lateral part, induces continuous for 10 h without sleep rebound neither amount nor power spectra. Optogenetic glutamatergic MPB neurons sleeping rats immediately wake mediated by basal...
Optochemistry, an emerging pharmacologic approach in which light is used to selectively activate or deactivate molecules, has the potential alleviate symptoms, cure diseases, and improve quality of life while preventing uncontrolled drug effects. The development in-vivo applications for optochemistry render brain cells photoresponsive without relying on genetic engineering been progressing slowly. nucleus accumbens (NAc) a region regulation slow-wave sleep (SWS) through integration...
Sleep–wake behavior is controlled by a wide range of neuronal populations in the mammalian brain. Although ventral midbrain/pons (VMP) area suggested to participate sleep–wake regulation, mechanisms have remained unclear. Here, we found that nonspecific cell ablation or selective GABAergic neurons expressing diphtheria toxin fragment A VMP male mice induced large increase wakefulness lasted at least 4 weeks. In contrast, dopaminergic had little effect on wakefulness. Chemogenetic inhibition...
Scope Zinc is an essential mineral that plays important role in the body. We previously reported orally feeding zinc‐enriched yeast to mice induces nonrapid‐eye‐movement sleep. In addition, astaxanthin, antioxidant abundant seafood such as salmon and krill, able chelate minerals may promote zinc absorption, which return also improve The purpose of our study was examine effect zinc‐rich astaxanthin‐containing food on sleep humans. Methods results conducted a randomized, double‐blinded,...
Abstract The dorsal raphe nucleus (DRN) is known to control aggressive behavior in mice. Here, we found that glutamatergic projections from the lateral habenula (LHb) DRN were activated male mice experienced pre-exposure a rival mouse (“social instigation”) resulting heightened intermale aggression. Both chemogenetic and optogenetic suppression of LHb-DRN projection blocked aggression after social instigation In contrast, inhibition this pathway did not affect basal levels behavior,...