A5011195208- Olfactory and Sensory Function Studies
- Biochemical Analysis and Sensing Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurobiology and Insect Physiology Research
- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Cancer Treatment and Pharmacology
- Circadian rhythm and melatonin
- Neuroscience of respiration and sleep
- Advanced Chemical Sensor Technologies
- Thermal Regulation in Medicine
- Photochromic and Fluorescence Chemistry
- Photoreceptor and optogenetics research
- Cancer Diagnosis and Treatment
- Retinal Development and Disorders
- Memory and Neural Mechanisms
- Liver Disease Diagnosis and Treatment
- Cancer Research and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Vascular Tumors and Angiosarcomas
- Single-cell and spatial transcriptomics
- Cardiac Ischemia and Reperfusion
- Genomic variations and chromosomal abnormalities
- Dietary Effects on Health
- Advanced Fluorescence Microscopy Techniques
Kansai Medical University
2015-2023
Japan Science and Technology Agency
2015
The University of Tokyo
1966-2012
Osaka Science Museum
2010
Tokyo University of Science
2002-2009
Osaka National Hospital
1971
Osaka Hospital
1971
Odor signals are conveyed from the olfactory bulb to cortex (OC) by mitral cells (MCs) and tufted (TCs). However, whether how two types of projection neuron differ in function axonal connectivity is still poorly understood. responses patterns were compared between MCs TCs mice visualizing axons electrophysiologically identified single neurons. demonstrated shorter onset latency for reliable than MCs. The response was maintained a wide range odor concentrations, whereas responded only strong...
Sensory information detected by the peripheral nervous system is represented as a topographic map in brain. It has long been thought that topography of determined graded positional cues are expressed target. Here, we analyzed pre-target axon sorting for olfactory formation mice. In sensory neurons, an guidance receptor, Neuropilin-1, and its repulsive ligand, Semaphorin-3A, complementary manner. We found expression levels Neuropilin-1 both projection sites axons. Olfactory neuron-specific...
In developing brains, activity-dependent remodeling facilitates the formation of precise neuronal connectivity. Synaptic competition is known to facilitate synapse elimination; however, it has remained unknown how different synapses compete with one another within a post-synaptic cell. Here, we investigate mitral cell in mouse olfactory bulb prunes all but primary dendrite during developmental process. We find that spontaneous activity generated essential. show strong glutamatergic inputs...
Significance It is now widely accepted that the range of pheromones control social behaviors are processed by both vomeronasal system (VNS) and main olfactory (MOS). However, functional contributions each subsystem in behavior remain unclear. Here, we showed mice with loss-of-function confined to dorsal MOS maintained innate odor recognition VNS activity, but failed demonstrate multiple male female behaviors. Functional dissociation enabled identification an MOS-mediated processing...
Innate behaviors are genetically encoded, but their underlying molecular mechanisms remain largely unknown. Predator odor 2,4,5-trimethyl-3-thiazoline (TMT) and its potent analog 2-methyl-2-thiazoline (2MT) believed to activate specific odorant receptors elicit innate fear/defensive in naive mice. Here, we conduct a large-scale recessive genetics screen of ethylnitrosourea (ENU)-mutagenized We find that loss Trpa1, pungency/irritancy receptor, diminishes TMT/2MT snake skin-evoked responses....
The glomerular layer of the mammalian olfactory bulb (OB) forms odorant receptor (OR) maps. Each OR map is structurally and functionally compartmentalized into zones (dorsal ventral) domains (DI DII in dorsal zone). We previously reported that glomeruli with similar molecular receptive range properties formed feature clusters at stereotypical positions rat OB. However, spatial arrangement regard to has not been systematically examined. In this study, we optically mapped within domain zone...
In rodents, the olfactory epithelium (OE) can be divided into four topographically distinct zones, and each member of odorant receptor ( OR ) gene family is expressed only in one particular zone. To study functional significance zonal structure OE, we searched for genes a zone‐specific manner by using differential display method. Among clones isolated from rat characterized novel protein termed O‐MACS, medium‐chain acyl‐CoA synthetase family. The o‐macs encodes 580 amino acids, sharing...
Abstract The neural mechanisms of fear-associated thermoregulation remain unclear. Innate fear odor 2-methyl-2-thiazoline (2MT) elicits rapid hypothermia and elevated tail temperature, indicative vasodilation-induced heat dissipation, in wild-type mice, but not mice lacking Trpa1–the chemosensor for 2MT. Here we report that Trpa1 −/− show diminished 2MT-evoked c-fos expression the posterior subthalamic nucleus (PSTh), external lateral parabrachial subnucleus (PBel) solitary tract (NTS)....
Abstract Innate fear intimately connects to the life preservation in crises, although this relationships is not fully understood. Here, we report that presentation of a supernormal innate inducer 2-methyl-2-thiazoline (2MT), but learned stimuli, induced robust systemic hypothermia/hypometabolism and suppressed aerobic metabolism via phosphorylation pyruvate dehydrogenase, thereby enabling long-term survival lethal hypoxic environment. These responses exerted potent therapeutic effects...
Abstract Similar to fingerprints, humans have unique, genetically determined body odours. In case of urine, the odour can change due variations in diet as well upon infection or tumour formation. We investigated use mice a manner similar “sniffer dogs” detect changes urine patients with bladder cancer. measured discrimination thresholds Y-maze, using mixtures from cancer (Stage I) and healthy volunteers (dietary variations) occult blood- antibiotic drug metabolite-modulated samples....
Abstract Thiazoline-related innate fear-eliciting compounds (tFOs) orchestrate hypothermia, hypometabolism, and anti-hypoxia, which enable survival in lethal hypoxic conditions. Here, we show that most of these effects are severely attenuated transient receptor potential ankyrin 1 ( Trpa1 ) knockout mice. TFO-induced hypothermia involves the -mediated trigeminal/vagal pathways non- olfactory pathway. TFOs activate -positive sensory projecting from trigeminal vagal ganglia to spinal nucleus...
Abstract Enantiomeric pairs of mirror-image molecular structures are difficult to resolve by instrumental analyses. The human olfactory system, however, discriminates (−)-wine lactone from its (+)-form rapidly within seconds. To gain insight into receptor coding enantiomers, we compared behavioural detection and discrimination thresholds wild-type mice with those ΔD in which all dorsal receptors genetically ablated. Surprisingly, displayed an exquisite “supersensitivity” enantiomeric wine...
We identified a stomatin-related olfactory protein (SRO) that is specifically expressed in sensory neurons (OSNs). The mouse sro gene encodes polypeptide of 287 amino acids with calculated molecular weight 32 kDa. SRO shares 82% sequence similarity the murine stomatin, 78% Caenorhabditis elegans MEC-2, and 77% C. UNC-1. Unlike other stomatin-family genes, transcript was present only OSNs main epithelium. No expression seen vomeronasal neurons. abundant most apical dendrites OSNs, including...
SUMMARY In developing brains, activity-dependent remodeling facilitates the formation of precise neuronal connectivity. Synaptic competition is known to facilitate synapse elimination; however, it has remained unknown how different synapses compete each other within a postsynaptic cell. Here we investigate mitral cell in olfactory bulb prunes all but one primary dendrite during developmental process. We find that spontaneous activity generated essential. show strong glutamatergic inputs...
Summary Miniaturized fluorescence microscopes are becoming more important for deciphering the neural codes underlying various brain functions. With gradient index (GRIN) lenses, these devices enable recording neuronal activity in deep structures. However, to minimize any damage tissues and local circuits, diameter of GRIN lens should be 0.5–1 mm, resulting a small field view. Volumetric imaging capability might increase number neurons imaged through lenses considering three-dimensional (3D)...
Abstract Aims Cardiac ischaemia/reperfusion (I/R) injury remains a critical issue in the therapeutic management of ischaemic heart failure. Although mild hypothermia has protective effect on cardiac I/R injury, more rapid and safe methods that can obtain similar results to therapy are required. 2‐Methyl‐2‐thiazoline (2MT), an innate fear inducer, causes resulting resistance hypoxia cutaneous or cerebral injury. The aim this study is demonstrate systemically administered 2MT elucidate...
Summary Therapeutic hypothermia protects the brain after cardiopulmonary arrest. Innate fear has evolved to orchestrate protective effects in life-threatening situations. Thus, strong perception may induce a specialized life-protective metabolism based on hypothermia/hypometabolism; however, such phenomena and their inducers are yet be elucidated. Here, we report that thiazoline-related odors (tFOs), which TRPA1 agonists robust innate mice, induced hibernation-like systemic...
The original
Sensory signals are critical to perform adaptive social behavior. During copulation, male mice emit ultrasonic vocalizations (USVs). Our previous studies have shown that female exhibit approach behavior toward sound sources of USVs and that, after being exposed a pheromone, exocrine gland-secreting peptide 1 (ESP1), exhibited preference particular type USVs. These findings suggest modulate courtship However, it remains unclear which brain regions what cell types neurons involved in neuronal...
We hypothesized that circulatory and jejunal mucosal blood flow would improve after 2-methyl-2thiazoline (2MT) administration in endotoxic shock. This study aimed to evaluate changes systemic circulation superior mesenteric venous (SMV) tissue of the intestinal vascular system over time 2MT rabbits with created four groups (n = 6 each): control group, LPS (1 mg/kg) (80 LPS-2MT group. As indicators circulation, we measured MAP, heart rate, cardiac index, lactic acid level, SMV flow, every 30...