A5007274064- Pancreatic function and diabetes
- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Cardiac electrophysiology and arrhythmias
- Adenosine and Purinergic Signaling
- Ion Channels and Receptors
- Dermatology and Skin Diseases
- Blood Coagulation and Thrombosis Mechanisms
- Prostate Cancer Treatment and Research
- Connective tissue disorders research
- Neonatal Respiratory Health Research
- Neuropeptides and Animal Physiology
- Hormonal and reproductive studies
- Connexins and lens biology
- Heart Failure Treatment and Management
- Cancer Cells and Metastasis
- Prostate Cancer Diagnosis and Treatment
- Pain Mechanisms and Treatments
- Glioma Diagnosis and Treatment
- Neuroscience of respiration and sleep
- Bone health and treatments
- Atrial Fibrillation Management and Outcomes
- Urological Disorders and Treatments
- Vitamin K Research Studies
- Protease and Inhibitor Mechanisms
Kansai Medical University
2010-2024
Japan Tobacco (Japan)
2002-2021
Institute of Biomedical Science
2021
Institute of Molecular Biology and Biophysics
2016
Nagasaki Medical Center
2002-2014
University of Copenhagen
2012-2013
Yokohama City University
2013
Hyogo Prefectural Nishinomiya Hospital
2013
Osaka University Hospital
2013
Gunma University Hospital
2013
Gut epithelial organoids are routinely used to investigate intestinal biology; however, current culture methods not amenable genetic manipulation, and it is difficult generate sufficient numbers for high-throughput studies. Here, we present an improved system of human induced pluripotent stem cell (iPSC)-derived involving four methodological advances. (1) We adopted a lentiviral vector readily establish optimize conditioned medium organoid culture. (2) obtained from iPSCs more efficiently by...
Targeting immune checkpoint proteins has recently gained substantial attention due to the dramatic success of this strategy in clinical trials for some cancers. Inducible T-cell co-stimulator ligand (ICOSLG) is a member B7 family regulatory ligands, expression which cancer implicated disease progression regulation antitumor adaptive immunity. Although aberrant ICOSLG been reported glioma cells, underlying mechanisms that promote glioblastoma (GBM) remain elusive.Here, we investigated causal...
Abstract Janus kinases ( JAK s) are required for several inflammatory cytokine signalling pathways and implicated in the pathogenesis of chronic dermatitis, including atopic dermatitis psoriasis. inhibitors therefore promising therapeutic candidates dermatitis. In this study, we evaluated effects novel inhibitor JTE ‐052 on responses associated with compared its profile those conventional agents rodent models inhibited Th1‐, Th2‐ Th17‐type human T cells mast vitro. Oral administration skin...
Visceral fat accumulation as observed in Crohn's disease and obesity is linked to chronic gut inflammation, suggesting that of adipocytes can trigger local inflammatory signaling. However, direct interactions between intestinal epithelial cells (IECs) have not been investigated, part because IEC physiology difficult replicate culture. In this study, we originally prepared intact, polarized, cytokine responsive monolayers from primary or induced pluripotent stem cell-derived organoids by...
Potassium channels play a vital role in maintaining the membrane potential and driving force for anion secretion epithelia. In pancreatic ducts, which secrete bicarbonate-rich fluid, identity of K(+) has not been extensively investigated. this study, we investigated molecular basis functional rodent human ducts (Capan-1, PANC-1, CFPAC-1) using electrophysiological techniques. RT-PCR analysis revealed mRNAs KCNQ1, KCNH2, KCNH5, KCNT1, KCNT2, as well KCNN4 coding following channels: KVLQT1;...
We evaluated the role of TRPV1 in bladder overactivity based on afferent nerve firing and urodynamic parameters using selective antagonist JTS-653.We effects JTS-653 increased pelvic discharge intravesical pressure induced by infusion 100 μM capsaicin anesthetized rats. The 30 nM resiniferatoxin or 0.2% acetic acid, normal activity were cystometry conscious carbachol contraction investigated muscle strips.JTS-653 significantly suppressed increase pressure. Intravesical acid decreased...
Adenosine modulates a wide variety of biological processes via adenosine receptors. In the exocrine pancreas, regulates transepithelial anion secretion in duct cells and is considered to play role acini-to-duct signaling. To identify functional receptors Cl− channels important for secretion, we herein performed experiments on Capan-1, human pancreatic cell line, using open-circuit Ussing chamber gramicidin-perforated patch-clamp techniques. The luminal addition increased negative potential...
Abstract Thiazoline-related innate fear-eliciting compounds (tFOs) orchestrate hypothermia, hypometabolism, and anti-hypoxia, which enable survival in lethal hypoxic conditions. Here, we show that most of these effects are severely attenuated transient receptor potential ankyrin 1 ( Trpa1 ) knockout mice. TFO-induced hypothermia involves the -mediated trigeminal/vagal pathways non- olfactory pathway. TFOs activate -positive sensory projecting from trigeminal vagal ganglia to spinal nucleus...
The age- and gender-related changes in extracellular matrix components (elastin, elastin cross-links, fibrillin, collagen glycoprotein) mineral (calcium, Ca; phosphorus, P) human lumbar yellow ligaments were investigated using samples obtained from surgical specimens. (Ca contents increased with ageing (r = 0.703 r 0.772, respectively), whereas the of tended to decrease (elastin -0.261, cross-links -0.213, fibrillin 0.494; -0.322 glycoprotein -0.143). Comparison male female groups revealed...
Although Ca 2+ -activated K + (K ) channels distinct from maxi-K have been suggested to contribute muscarinically stimulated currents in salivary acinar cells, the molecular nature of is unclear. Using electrophysiological and RT-PCR techniques, we now investigated involvement SK4/IK1-like native bovine parotid (BPA) cells. -dependent efflux perfused tissues was not inhibited by a channel blocker, tetraethylammonium (TEA). Whole cell recordings BPA cells showed TEA-insensitive conductance,...
Proteasomes are the multisubunit protease involved in generation of peptides presented by MHC class I molecules. When cells stimulated with IFN-gamma, two MHC-encoded subunits, low Mr polypeptide 2 (LMP2) and LMP7, incorporated into proteasomal complex displacing housekeeping designated Y X, respectively. Recent evidence indicates that besides LMP2/Y LMP7/X there is another pair structurally similar beta-type MECL1 Z, whose expression regulated reciprocally IFN-y. In present study we...
SK4/IK1 encodes an intermediate conductance, Ca 2+ -activated K + channel and fulfills a variety of physiological functions in excitable nonexcitable cells. Although recent studies have provided evidence for the presence channels salivary acinar cells, regulatory mechanisms function remain unknown these Using molecular electrophysiological techniques, we examined whether cytosolic ATP-dependent regulation native SK4/IK1-like activity would involve endogenous cAMP-dependent protein kinase...
Short-acting oral calcilytics, calcium-sensing receptor (CaSR) antagonists, have been considered as alternatives for parathyroid hormone (PTH), an injectable bone anabolic drug used in the treatment of osteoporosis. Previously, we identified aminopropandiol 1, which transiently stimulated endogenous PTH secretion rats. However, inhibition cytochrome P450 (CYP) 2D6 and low bioavailability 1 remain to be solved. Attempts change physicochemical properties highly lipophilic amine by introduction...
We describe herein a patient who presented with immunoglobulin G4-related disease (IgG4-RD) involving the testis and prostate as well submandibular glands. Massive infiltration of IgG4-expressing plasma cells was observed in tissues. Serum concentrations B cell activating factor belonging to tumor necrosis family (BAFF) were elevated parallel serum IgG4 concentrations, BAFF-receptor (BAFF-R)-expressing BAFF-expressing lymphoid around ectopic foci affected urogenital To date, testicular...
Sudomotor function was studied based on sympathetic skin responses (SSRs) in 87 patients with various types of cerebellar degeneration. Evaluations orthostatic hypotension and the R-R interval variation were also performed these patients. The respective incidences SSR abnormality 44 (50.6%) 39 (44.8%) 87. Fourteen percent abnormal SSRs did not have hypotension, but 8% normal hypotension. Sympathetic most Shy-Drager syndrome, sporadic olivopontocerebellar atrophy (OPCA), striatonigral...
Transient receptor potential vanilloid 1 (TRPV1) activation in peripheral sensory nerve is known to be associated with various pain-related diseases, thus TRPV1 has been the focus as a target for drug discovery. In this study, we characterized pharmacological profiles of (3<i>S</i>)-3-(hydroxymethyl)-4-(5-methylpyridin-2-yl)-<i>N</i>-[6-(2,2,2-trifluoroethoxy)pyridin-3-yl]-3,4-dihydro-2<i>H</i>-benzo[<i>b</i>][1,4]oxazine-8-carboxamide (JTS-653), novel antagonist. JTS-653 displaced...
Short-chain fatty acids (SCFAs), such as acetate, propionate, and butyrate, are synthesized from dietary carbohydrates by colonic bacterial fermentation. These SCFAs supply energy, suppress cancer, affect ion transport. However, their roles in transport regulation the intracellular environment remain unknown. In order to elucidate of SCFAs, we measured short-circuit currents (ISC) performed RT-PCR immunohistochemical analyses transporters rat rectal colon. The application 30 mM butyrate...