A5102745434- Inflammasome and immune disorders
- Immune Response and Inflammation
- interferon and immune responses
- Oral microbiology and periodontitis research
- Fluid Dynamics and Vibration Analysis
- Endodontics and Root Canal Treatments
- Immune Cell Function and Interaction
- Fluid Dynamics and Turbulent Flows
- Dental Radiography and Imaging
- Particle Dynamics in Fluid Flows
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Axon Guidance and Neuronal Signaling
- Heme Oxygenase-1 and Carbon Monoxide
- Immune cells in cancer
- Macrophage Migration Inhibitory Factor
- T-cell and B-cell Immunology
- Adenosine and Purinergic Signaling
- Probiotics and Fermented Foods
- Bone Metabolism and Diseases
- Herpesvirus Infections and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Advanced Frequency and Time Standards
- Underground infrastructure and sustainability
- Sinusitis and nasal conditions
Osaka University
2004-2024
Osaka Metropolitan University
2023
The University of Tokyo
2000-2020
Chiba University
2016-2020
Kitasato University
2020
RIKEN Center for Integrative Medical Sciences
2016
Integrated Laboratory Systems, Inc.
2015
Osaka International University
2011-2012
CD103(+) dendritic cells (DCs) are the major conventional DC population in intestinal lamina propria (LP). Our previous report showed that a small number of LP could be classified into four subsets based on difference CD11c/CD11b expression patterns: CD11c(hi)CD11b(lo) DCs, CD11c(hi)CD11b(hi) CD11c(int)CD11b(int) macrophages, and CD11c(int)CD11b(hi) eosinophils. The which CD103(+), specifically express TLR5 induce differentiation naive B IgA(+) plasma cells. These DCs also mediate...
High-dose ionizing radiation induces severe DNA damage in the epithelial stem cells small intestinal crypts and causes gastrointestinal syndrome (GIS). Although tumour suppressor p53 is a primary factor inducing death of crypt with damage, its essential role maintaining genome stability means inhibiting to prevent GIS not viable strategy. Here we show that innate immune receptor Toll-like 3 (TLR3) critical for pathogenesis GIS. Tlr3(-/-) mice substantial resistance owing significantly...
Gut epithelial organoids are routinely used to investigate intestinal biology; however, current culture methods not amenable genetic manipulation, and it is difficult generate sufficient numbers for high-throughput studies. Here, we present an improved system of human induced pluripotent stem cell (iPSC)-derived involving four methodological advances. (1) We adopted a lentiviral vector readily establish optimize conditioned medium organoid culture. (2) obtained from iPSCs more efficiently by...
Candida albicans can enter skeletal tissue through a skin wound in an immunocompromised host or by contamination during orthopedic surgery. Such osteomyelitis is accompanied severe pain and bone destruction. It established that nociceptor innervation occurs bone, but the mechanisms of nociceptive modulation fungal inflammation remain unclear. In this study, we show C. stimulates Nav1.8-positive nociceptors via β-glucan receptor Dectin-1 to induce calcitonin gene-related peptide (CGRP). This...
Radiation-induced intestinal fibrosis (RIF) is a serious complication after abdominal radiotherapy for pelvic tumor or peritoneal metastasis. Herein, we show that RIF mediated by eosinophil interactions with α-smooth muscle actin-positive (α-SMA+) stromal cells. Abdominal irradiation caused especially in the submucosa (SM) of small intestine, which was associated excessive accumulation eosinophils both human and mouse. Eosinophil-deficient mice showed markedly ameliorated RIF, suggesting...
Candida albicans infection can cause skin, vulvar, or oral pain. Despite the obvious algesic activity of C. albicans, molecular mechanisms fungal nociception remain largely unknown. Here we show that albicans-specific signaling pathway led to severe mechanical allodynia. We discovered albicans-derived β-glucan stimulated nociceptors depending on Dectin-1, and two pathways in inflammatory The major operates via Dectin-1-mediated ATP-P2X3/P2X2/3 axis through intercellular relationships between...
Abstract The gut is an extremely complicated ecosystem where micro-organisms, nutrients and host cells interact vigorously. Although the function of intestine its barrier system weakens with age, some probiotics can potentially prevent age-related intestinal dysfunction. Lactobacillus delbrueckii subsp. bulgaricus 2038 Streptococcus thermophilus 1131, which are constituents LB81 yogurt, representative probiotics. However, it unclear whether their long-term intake has a beneficial influence...
Abstract Laminar separation bubbles formed on NACA 0012 aerofoil near the onset of a stall were investigated to clarify behaviour laminar bubble. Measurements done at chord Reynolds number 1·3 × 10 5 . Mean velocity measurements indicate that long bubble about 35% length is α = 11·5° after short burst occurred. However, instantaneous flow visualisation picture indicates strongly oscillating this angle attack. The phase averaging technique has been applied analyse behaviour. results between...
The participation of bacterial biofilms in the over‐filled gutta‐percha points associated with refractory periapical periodontitis has recently been reported. This study investigated initial biofilm‐forming ability root canal isolates ( Enterococcus faecalis, Streptococcus sanguis , Strep. intermedius pyogenes Staphylococcus aureus Fusobacterium nucleatum Propionibacterium acnes Porphyromonas gingivalis and Prevotella intermedia ) on vitro . Each strain was suspended 100% cell culture medium...
Abstract AbstractToll-like receptor 5 (TLR5), a sensor for bacterial flagellin, mounts innate and adaptive immune responses, has been implicated in infectious diseases, colitis metabolic syndromes. Although TLR5 is believed to belong cell surface TLRs, expression never verified. Moreover, it remained unclear which types of cells express contribute flagellin-dependent responses. In this study we established an anti-mouse monoclonal antibody studied the on cells. The macrophage line J774...
Herpes simplex virus-1 (HSV-1) is the most common cause of sporadic viral encephalitis, which can be lethal or result in severe neurological defects even with antiviral therapy. While HSV-1 causes encephalitis spite HSV-1-specific humoral and cellular immunity, mechanism by evades immune system central nervous (CNS) remains unknown. Here we describe a strategy avoids targeting CNS. The UL13 kinase promotes evasion CD8+ T cell accumulation infection sites downregulating expression attractant...
Oral immunotherapy (OIT) is an effective approach to controlling food allergy. Although the detailed molecular and cellular mechanisms of OIT are unknown currently, they must be understood advance treatment allergic diseases in general. To elucidate OIT, especially during immunological transition from desensitization allergy regulation, we generated a clinical murine model used it examine events OIT. We found that mice completed successfully, desensitized mast cells (MCs) showed functionally...
p53 is a critical host cell factor in the cellular response to broad range of stress factors. We recently reported that required for efficient herpes simplex virus 1 (HSV-1) replication culture. However, defined role HSV-1 and pathogenesis vivo remains elusive. In this study, we examined effects on infection using p53-deficient mice. Following intracranial inoculation, knockout reduced viral brains mice led significantly rates mortality due encephalitis. These results suggest an important...
Nociceptors can fine-tune local or systemic immunity, but the mechanisms of nociceptive modulation in endotoxic death remain largely unknown. Here, we identified C-type lectin Reg3γ as a nociceptor-enriched hormone that protects host from death. During endotoxemia, nociceptor-derived penetrates brain and suppresses expression microglial indoleamine dioxygenase 1, critical enzyme kynurenine pathway, via Extl3-Bcl10 axis. Endotoxin-administered nociceptor-null mice nociceptor-specific...
Incidence of type I allergies, such as hay fever, is continuously increasing in developed countries, including Japan. Type allergies are triggered by chemical mediators, histamine, which released via immunoglobulin E (IgE)-mediated mast cell degranulation. Therefore, medications inhibiting the synthesis, release, and receptor binding these mediators commonly used to manage allergy symptoms. As self-care disease prevention practices gaining attention worldwide, regular consumption food...
Nod-like receptor family pyrin domain-containing 3 (NLRP3) is a cytosolic innate immune that senses organelle dysfunction induced by various stimuli, such as infectious, environmental, metabolic and drug stresses. Upon activation, NLRP3 forms an inflammasome with its adaptor protein apoptosis-associated speck-like containing caspase recruitment domain (ASC) caspase-1, to trigger the release of inflammatory cytokines. The development effective anti-inflammatory drugs targeting in high demand...
Abstract Laminar separation bubbles formed on NACA 0012 aerofoil near the onset of a stall were investigated to clarify behaviour laminar bubble. Measurements done at chord Reynolds number 1·3 × 10 5 . Mean velocity measurements indicate that long bubble about 35% length is α = 11·5° after short burst occurred. However, instantaneous flow visualisation picture indicates strongly oscillating this angle attack. The phase averaging technique has been applied analyse behaviour. results between...