A5012898958- Immune Response and Inflammation
- interferon and immune responses
- Immune cells in cancer
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Mosquito-borne diseases and control
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Immunotherapy and Immune Responses
- Inflammasome and immune disorders
- IL-33, ST2, and ILC Pathways
- Genomics and Chromatin Dynamics
- Viral Infections and Vectors
- NF-κB Signaling Pathways
- Influenza Virus Research Studies
- Single-cell and spatial transcriptomics
- Eosinophilic Esophagitis
- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- RNA Interference and Gene Delivery
- Gene Regulatory Network Analysis
- Advanced Fluorescence Microscopy Techniques
- T-cell and B-cell Immunology
- Cancer-related molecular mechanisms research
- Immune responses and vaccinations
National Institute of Advanced Industrial Science and Technology
2016-2024
Tokyo Institute of Technology
2003-2021
Osaka University
2009-2018
Osaka International University
2008-2018
Ministry of Defense
2009-2016
Tohoku University
2014
Ube Frontier University
2011
University of Florida
2010
Soka University
2009
Microbial Chemistry Research Foundation
2009
RNA virus infection is recognized by retinoic acid-inducible gene (RIG)-I–like receptors (RLRs), RIG-I, and melanoma differentiation–associated 5 (MDA5) in the cytoplasm. RLRs are comprised of N-terminal caspase-recruitment domains (CARDs) a DExD/H-box helicase domain. The third member RLR family, LGP2, lacks any CARDs was originally identified as negative regulator signaling. In present study, we generated mice lacking LGP2 found that required for RIG-I– MDA5-mediated antiviral responses....
Type I interferons (IFNs) are important for host defense from viral infections, acting to restrict production in infected cells and promote antiviral immune responses. However, the type IFN system has also been associated with severe lung inflammatory disease response respiratory syncytial virus (RSV). Which produce IFNs upon RSV infection how this directs responses virus, potentially results pathological inflammation, is unclear. Here, we show that alveolar macrophages (AMs) major source of...
Increased type I interferon (IFN-I) production and IFN-stimulated gene (ISG) expression are linked to the pathogenesis of systemic lupus erythematosus (SLE). Although mechanisms responsible for dysregulated IFN-I in SLE remain unclear, autoantibody-mediated uptake endogenous nucleic acids is thought play a role. 2,6,10,14-tetramethylpentadecane (TMPD; also known as pristane) induces lupus-like disease mice characterized by immune complex nephritis with autoantibodies DNA ribonucleoproteins....
All arthropod-borne flaviviruses generate a short noncoding RNA (sfRNA) from the viral 3′ untranslated region during infection due to stalling of cellular 5′-to-3′ exonuclease XRN1. We show here that formation sfRNA also inhibits XRN1 activity. Cells infected with Dengue or Kunjin viruses accumulate uncapped mRNAs, decay intermediates normally targeted by repression resulted in increased overall stability mRNAs flavivirus-infected cells. Importantly, mutant virus cannot form but replicates...
ABSTRACT We previously showed that a noncoding subgenomic flavivirus RNA (sfRNA) is required for viral pathogenicity, as mutant West Nile virus (WNV) deficient in sfRNA production replicated poorly wild-type mice. To investigate the possible immunomodulatory or immune evasive functions of sfRNA, we utilized mice and cells elements type I interferon (IFN) response. Replication WNV was rescued lacking regulatory factor 3 (IRF-3) IRF-7 alpha/beta receptor (IFNAR), suggesting contribution...
ABSTRACT Chikungunya virus (CHIKV) infections can produce severe disease and mortality. Here we show that CHIKV infection of adult mice deficient in interferon response factors 3 7 (IRF3/7 −/− ) is lethal. Mortality was associated with undetectable levels alpha/beta (IFN-α/β) serum, ∼50- ∼10-fold increases IFN-γ tumor necrosis factor (TNF), respectively, increased replication, edema, vasculitis, hemorrhage, fever followed by hypothermia, oliguria, thrombocytopenia, raised hematocrits. These...
Abstract Fungal β-glucan, such as curdlan, triggers antifungal innate immune responses well shaping adaptive responses. In this study, we identified a key pathway that couples curdlan to Curdlan promoted the production of proinflammatory cytokine IL-1β by dendritic cells and macrophages through NLRP3 inflammasome. Stimulation with Candida albicans Saccharomyces cerevisiae also triggered inflammasome-mediated production. vivo, was required for efficient Ag-specific Ab when used an adjuvant,...
Microbes activate pattern recognition receptors to initiate adaptive immunity. T cells affect early innate inflammatory responses viral infection, but both activation and suppression have been demonstrated. We identify a novel role for B in the immune response during bacterial sepsis. demonstrate that Rag1−/− mice display deficient reduced survival Interestingly, cell–deficient or anti-CD20 cell–depleted mice, not α/β decreased cytokine chemokine production after Both treatment of with serum...
Rare, circulating dendritic cells differentially shape the immunogenicity mechanisms for protection against H1N1 influenza.
High-dose ionizing radiation induces severe DNA damage in the epithelial stem cells small intestinal crypts and causes gastrointestinal syndrome (GIS). Although tumour suppressor p53 is a primary factor inducing death of crypt with damage, its essential role maintaining genome stability means inhibiting to prevent GIS not viable strategy. Here we show that innate immune receptor Toll-like 3 (TLR3) critical for pathogenesis GIS. Tlr3(-/-) mice substantial resistance owing significantly...
Candida albicans can enter skeletal tissue through a skin wound in an immunocompromised host or by contamination during orthopedic surgery. Such osteomyelitis is accompanied severe pain and bone destruction. It established that nociceptor innervation occurs bone, but the mechanisms of nociceptive modulation fungal inflammation remain unclear. In this study, we show C. stimulates Nav1.8-positive nociceptors via β-glucan receptor Dectin-1 to induce calcitonin gene-related peptide (CGRP). This...
ABSTRACT Toll-like receptors (TLRs) and retinoic acid-inducible gene I-like helicases (RLHs) are two major machineries recognizing RNA virus infection of innate immune cells. Intracellular signaling for TLRs RLHs is mediated by their cytoplasmic adaptors, i.e., MyD88 or TRIF IPS-1, respectively. In the present study, we investigated contributions to cytotoxic T-lymphocyte (CTL) response using lymphocytoid choriomeningitis (LCMV) as a model virus. The generation virus-specific T lymphocytes...
Abstract Bone marrow (BM) hematopoietic stem and progenitor cells (HSPCs) can be activated by type I IFNs, TLR agonists, viruses, bacteria to increase hematopoiesis. In this study, we report that endotoxin treatment in vivo induces TLR4, MyD88, Toll/IL-1 resistance domain-containing adaptor-inducing IFN-β (TRIF)-dependent expansion of BM HSPCs. Bacterial infection Staphylococcus aureus or cecal ligation puncture also HSPC expansion, but TRIF, IFN, cytokine, PG, oxidative stress pathways are...