A5024695820- Pancreatic and Hepatic Oncology Research
- Gastric Cancer Management and Outcomes
- Colorectal Cancer Surgical Treatments
- Metastasis and carcinoma case studies
- Colorectal Cancer Treatments and Studies
- Cancer Cells and Metastasis
- Esophageal and GI Pathology
- Colorectal and Anal Carcinomas
- Pancreatitis Pathology and Treatment
- Nutrition and Health in Aging
- Esophageal Cancer Research and Treatment
- Gastrointestinal Tumor Research and Treatment
- Cancer Immunotherapy and Biomarkers
- Intraperitoneal and Appendiceal Malignancies
- Gallbladder and Bile Duct Disorders
- Sepsis Diagnosis and Treatment
- DNA and Nucleic Acid Chemistry
- Gastrointestinal disorders and treatments
- RNA and protein synthesis mechanisms
- Immune Response and Inflammation
- Lymphoma Diagnosis and Treatment
- Cancer Research and Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Lung Cancer Treatments and Mutations
- Neuroendocrine Tumor Research Advances
Shiga University of Medical Science
2016-2025
Ritsumeikan University
2021-2025
RIKEN
2024
Shiga University of Medical Science Hospital
2024
The University of Texas MD Anderson Cancer Center
2014-2024
Osaka University
1976-2023
Iwate Medical University
2016
Fukuoka University
1998-2015
Shimane University Hospital
2013
American Gastroenterological Association
2012
The tumor microenvironment in pancreatic ductal adenocarcinoma (PDAC) involves a significant accumulation of fibroblasts as part the host response to cancer. Using single-cell RNA sequencing, multiplex immunostaining, and several genetic mouse models, we identify carcinoma-associated (CAF) with opposing functions PDAC progression. Depletion fibroblast activation protein (FAP)+ CAFs results increased survival, contrast depletion alpha smooth muscle actin (αSMA)+ CAFs, which leads decreased...
A synthetic gene for human epidermal growth factor (hEGF) was joined to a sequence encoding the signal peptide of Escherichia coli alkaline phosphatase. This hybrid placed under control phosphatase (phoA) promoter in recombinant plasmid, which used transfect E. coli. The protein that expressed host cells conditions phosphate limitation processed accurately during secretion process, and mature hEGF recovered periplasmic fraction. On other hand, no EGF detected space when not accompanied by...
Skin wound repair is essential for organismal survival and failure of which leads to non-healing wounds, a leading health issue worldwide. However, mechanistic understanding chronic wounds remains major challenge due lack appropriate genetic mouse models. αSMA+ myofibroblasts, unique class dermal fibroblasts, are associated with cutaneous healing but their precise function unknown. We demonstrate that depletion myofibroblasts pleiotropic defects, including reepithelialization granulation,...
Abstract NK cells play essential roles in eliminating virally infected and tumor cells. Polyinosinic-polycytidylic acid (poly I:C), a double-stranded RNA analog recognized by melanoma-differentiation associated gene 5 (MDA5) TLR3, activates vivo. MDA5 TLR3 signal through distinct adaptor molecules, IFN-promoter stimulator-1 (IPS-1) Toll/IL-1R domain-containing inducing IFN-β (TRIF), respectively. However, it remains unclear how are activated poly I:C In this study, we demonstrate that the...
The invasive tumour front may provide prognostic information. We examined the relationship between presence of cancer stem cells (CSCs) at and prognosis in gastric (GC). CD44 is a CSC marker; accordingly, standard (CD44s), variant-6 (CD44v6), variant-9 (CD44v9) expression were 123 resected primary GCs clinical significance CSCs was analysed. Thirteen (10.6%), 79 (64.2%), 47 (38.2%) CD44s-, CD44v6-, CD44v9-positive, respectively. Patients with CD44-positive had significantly poorer...
The present study aimed to determine the clinical value of serum procalcitonin (PCT) level in predicting postoperative infections after hepatectomy. Medical records 301 consecutive patients who underwent hepatectomy were retrospectively reviewed. We divided into infection-positive and infection-negative groups. investigated changes perioperative inflammatory markers such as C-reactive protein (CRP) PCT level. Associations between infectious complications evaluated identify predictive factors...
Sodium zirconium cyclosilicate (SZC) binds with potassium in the gastrointestinal tract and increases fecal excretion. Since SZC is a novel non-swelling drug, risk of intestinal perforation not mentioned package insert. We hereby report case sigmoid colon caused by hard stools severe hypokalemia patient suffering from advanced rectal cancer, who was taking SZC. A woman her 80s history primary biliary cirrhosis decompensated accompanied hyperkalemia administered The rushed to hospital on 23rd...
Postoperative peritoneal adhesion formation (PAF) is a distressing complication. Leptin, secreted by fat tissues, may be associated with PAF. However, the relationships between PAF and leptin are unclear. This study investigated impact of on after colorectal tumor resection. A cecum cauterization mouse model was used to investigate its association leptin. Mice score (AS) evaluated day 7 treatment. The clinical included patients who underwent elective resection temporary ileostomy April 2021...
Abstract This study presents the development of a piezo actuator-based fatigue testing machine specifically for miniature specimens. The machine’s specifications include maximum axial load 1 kN, frequency 15 Hz, and overall dimensions 1,174 mm × 370 270 (height length width). Finite element analysis was employed to design two types specimens with 22 length, 8 width, 2.2 thickness, each having gauge 4 diameters 1.6 1.8 mm. Preliminary indicated that fractures initiated in R-section specimen...
Objectives: The clinical significance of early administration pancrelipase after distal pancreatectomy (DP) has not been reported, and its effect on new-onset diabetes mellitus (DM) clarified. This study aimed to investigate the DP postoperative nutritional status, skeletal muscle mass, DM. Methods: We retrospectively reviewed medical records 76 patients who underwent DP. Delayed-release high-titer was administered daily, starting day 3 (EP group). Postoperative mass index (SMI), DM were...
IκBζ, encoded by Nfibiz , is a nuclear IκB-like protein harboring ankyrin repeats. IκBζ has been shown to regulate IL-6 production in macrophages and Th17 development T cells. However, the role of natural killer (NK) cells not be understood. In present study, we found that expression was rapidly induced response IL-18 NK cells, but Analysis Nfkbiz −/− mice revealed essential for IFN-γ cytotoxic activity IL-12 and/or stimulation. IL-12/IL-18–mediated gene induction profoundly impaired Whereas...
// Daiji Ikuta 1 , Toru Miyake Tomoharu Shimizu Hiromichi Sonoda Ken-Ichi Mukaisho 2 Aya Tokuda Tomoyuki Ueki Hiroyuki Sugihara and Masaji Tani Department of Surgery, Shiga University Medical Science, Shiga, Japan Molecular diagnostic Pathology, Correspondence to: Miyake, email: myk@belle.shiga-med.ac.jp Keywords: cancer-associated fibroblast; colorectal cancer; fibroblastic reticular cell; fibrosis; lymph node metastasis Received: December 23, 2017 Accepted: June 03, 2018 Published: July...
<b><i>Introduction:</i></b> Microbiomes play a vital role in the development and progression of cancer. The clinical status, including prognosis, genetic mutations, sensitivity to chemotherapy, differs depending on location colorectal cancer (CRC); however, association between gut microbiota CRC is not entirely understood. This study was conducted evaluate differences patients with according tumor. <b><i>Methods:</i></b> Fifty-six who underwent...
A RNA molecule has been synthesized that is identical in sequence to Escherichia coli tRNAfMet except it lacks the base modifications present E. tRNA. This was achieved by enzymatic joining of chemically oligonucleotides with chain lengths 3-10 which were phosphodiester or phosphotriester method. First, quarter molecules tRNA constructed fragments ligase. The 5'-quarter (bases 1-20) served as an acceptor reactions 3',5'-bisphosphorylated donor 21-34). 5'-half thus obtained treated...
Endeavoring to develop a method biosynthesize proteins substituted with nonprotein amino acids, we attempted the incorporation of L-2-aminohexanoic acid (Ahx) into human epidermal growth factor (hEGF). Escherichia coli YK537 strain harboring plasmid pTA1522, which has phoA promoter-phoA signal peptide-hEGF gene, was used. Cells were cultured first in high-phosphate medium and then, for induction hEGF-encoding transferred low-phosphate containing Ahx (0.25 mg/ml). hEGF Ahx-substituted hEGF,...
To investigate the impact of postoperative infection (PI), surgical site infection, and remote (RI), on long-term outcomes in patients with colorectal cancer (CRC).