Pancreatic ductal adenocarcinoma (PDAC) is presently one of the cancers with worst survival rates and least effective treatments. Moreover, total deaths due to PDAC are predicted increase in next 15 years. Therefore, novel insights into basic mechanism development therapies needed. characterized by a complex microenvironment, which cancer stromal cells release different molecules, such as ATP. ATP can be transported and/or exocytosed from active released dying necrotic core cancer. We...

Pancreatic ductal adenocarcinoma (PDAC) has one of the worst survival rates all cancers. ANO1 (TMEM16A) is a recently identified Ca(2+)-activated Cl(-) channel (CaCC) that upregulated in several tumors. Although was subject to extensive studies recent years, its pathophysiological function only been poorly understood. The aim present study establish significance PDAC behavior and demarcate roles from those volume-regulated anion (VRAC). We performed qPCR Western blot measurements on...

The energy providing substrate ATP can be released from various cells and act extracellularly to regulate the same or neighboring cells. However, pathway for release eliciting physiological stimulus are unclear. Recently, we showed that activates P2X P2Y purinergic receptors on pancreatic ducts. Thus, it was relevant ask whether upstream acini could source of releasable what might be. We used freshly prepared rat applied conventional luminescence measurements luciferin/luciferase reaction....

The ATP-gated receptor P2X7 (P2X7R) is involved in regulation of cell survival and has been interest cancer field. Pancreatic ductal adenocarcinoma (PDAC) a deadly new markers therapeutic targets are needed. PDAC characterized by complex tumour microenvironment, which includes pancreatic stellate cells (PSCs), potentially high nucleotide/side turnover. Our aim was to determine P2X7R expression function human vitro as well perform vivo efficacy study applying inhibitor an orthotopic xenograft...

Novel treatments for pancreatic ductal adenocarcinoma (PDAC) are severely needed. The aim of this work was to explore the roles H-lactate monocarboxylate transporters 1 and 4 (MCT1 MCT4) in PDAC cell migration invasiveness.Monocarboxylate transporter expression, localization, activity, function were explored human cells (MIAPaCa-2, Panc-1, BxPC-3, AsPC-1) normal epithelial (HPDE) cells, by quantitative polymerase chain reaction, immunoblotting, immunocytochemistry, lactate flux, migration,...

Calcium electroporation may offer a simple general tool for anticancer therapy. Transient permeabilization of cancer cell membranes created by applying short, high-voltage pulses in tumors enables high calcium influxes that trigger death. In this study, we compared the relative sensitivity different human tumor models and normal tissues to electroporation. Plasma membrane Ca2+-ATPase (PMCA) protein expression was confirmed vitro all lines primary dermal fibroblasts studied. types tested...

The pancreas is a complex gland performing both endocrine and exocrine functions. In recent years there has been increasing evidence that cells possess purinergic receptors, which influence processes such as insulin secretion epithelial ion transport. Most commonly, these have viewed separately. beta cells, stimulation of P2Y(1) receptors amplifies in the presence glucose. Nucleotides released from secretory granules could also contribute to autocrine/paracrine regulation pancreatic islets....

Abstract Electrochemical oxidation of (−)‐epigallocatechin gallate (EGCG), the main monomer flavanol found in green tea, has been investigated over a wide pH range at glassy‐carbon electrode using square‐wave voltammetry (SWV). Square‐wave voltammograms (EGC) and gallic acid have studied as well. The I–E profile EGCG, i.e. potentials current responses first second peak, is dependent. EGCG quasireversible process range, which was also confirmed by non‐linear relationship between peak currents...

The role played by anions in salivary secretion has been studied experiments on the isolated, perfused mandibular gland of rabbit, which perfusate Cl‐ and/or HCO3‐ were replaced other anions. Replacement with Br‐ had no significant effect rate, but replacement tested caused secretory rate to fall, 38% (I‐), 50% (NO3‐), 61% (isethionate, ise ‐), and 66% ( CH3SO4 respectively. ‐ or concentration rise up 4‐fold. I‐ seemed have little but, contrast ‐, entered saliva concentrations comparable...

1. A method is described for the isolation and vascular perfusion in vitro of mandibular gland rabbit. The perfusate a physiological salt solution containing glucose as only metabolic substrate. 2. During with solutions acetylcholine, secretes vigorously at rate manner similar to that seen vivo . Although becomes oedematous during perfusion, extent this oedema appears have no influence on secretory ability: perfused glands were capable functioning least 4 h, often more than 6 h. 3....

The mechanism by which pancreas secretes high HCO3- has not been fully resolved. This alkaline secretion, formed in pancreatic ducts, can be achieved transporting from serosa to mucosa or moving H+ the opposite direction. aim of present study was determine whether H+/K+-ATPases are expressed and functional human ducts proton pump inhibitors (PPIs) have effect on those. Here we show that gastric HKα1 HKβ subunits (ATP4A; ATP4B) non-gastric HKα2 (ATP12A) cells. Pumps similar localizations duct...

// Hannah Storck 1 , Benedikt Hild Sandra Schimmelpfennig Sarah Sargin Nikolaj Nielsen Angela Zaccagnino 4 Thomas Budde 2 Ivana Novak 3 Holger Kalthoff Albrecht Schwab Institut für Physiologie II, 48149 Münster, Gemany I, Section for Cell Biology and Physiology, Department of Biology, University Copenhagen, DK 2100 Denmark UKSH, Campus Kiel, Experimentelle Tumorforschung (IET), Sektion Molekulare Onkologie, D-24105 Germany Correspondence to: Schwab, email: aschwab@uni-muenster.de Keywords:...