A5048289057- Lung Cancer Treatments and Mutations
- Radiomics and Machine Learning in Medical Imaging
- Cancer Immunotherapy and Biomarkers
- Plasma Diagnostics and Applications
- Medical Imaging and Analysis
- Anatomy and Medical Technology
- RNA modifications and cancer
- Luminescence and Fluorescent Materials
- Surgical Simulation and Training
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Brain Tumor Detection and Classification
- Robotic Locomotion and Control
- Prosthetics and Rehabilitation Robotics
- Photoreceptor and optogenetics research
- Fibroblast Growth Factor Research
- Circadian rhythm and melatonin
- Atomic and Molecular Physics
- Magnetic confinement fusion research
- Memory and Neural Mechanisms
- Ferroptosis and cancer prognosis
- Augmented Reality Applications
- Cancer-related gene regulation
- Medical Image Segmentation Techniques
- Epigenetics and DNA Methylation
Nagoya University
1989-2024
International University of Health and Welfare
2021-2024
Keio University
2013-2023
Osaka Prefecture University
2016-2023
Shonan Kamakura General Hospital
2021-2023
Kansai Medical University
2017-2023
Keio University Hospital
2011-2021
Graduate School USA
2021
Osaka Health Science University
2021
Hokkaido University
2001-2019
Presence of Mycobacterium fortuitum in respiratory tracts usually indicates mere colonization or transient infection, whereas true pulmonary infection occurs patients with gastroesophageal disease. However, little is known about the diagnostic indications for M. and natural history disease.A 59-year-old man was referred to our hospital treatment against infection. Fifteen years before referral, he underwent total gastrectomy, after which experienced esophageal reflux symptoms. After patient...
Human lung cancer is a constellation of tumors with various histological and molecular properties. To build preclinical platform that covers this broad disease spectrum, we obtained specimens from multiple sources, including sputum circulating tumor cells, generated living biobank consisting 43 lines patient-derived organoids. The organoids recapitulated the hallmarks original tumors. Phenotypic screening niche factor dependency revealed EGFR mutations in adenocarcinoma are associated...
Abstract The discovery of somatic mutations in EGFR and development tyrosine kinase inhibitors (TKI) have revolutionized treatment for lung cancer. However, resistance to TKIs emerges almost all patients currently no effective is available. Here, we show that β-catenin essential EGFR-mutated cancers. β-Catenin was upregulated activated cells. Mutant preferentially bound phosphorylated β-catenin, leading an increase β-catenin–mediated transactivation, particularly cells harboring the...
Recent advances in the treatment of non-small cell lung cancer (NSCLC) with new agents require accurate histological subtyping at diagnosis to avoid higher risk an adverse response and obtain maximum therapeutic response. However, interobserver variability, tumor heterogeneity degree differentiation may affect decision concerning a pathological NSCLC. Therefore, aim this study was identify specific microRNAs (miRNAs) as standardized biomarkers high sensitivity specificity order distinguish...
Background. The immunohistochemical detection of aldosterone synthase (CYP11B2) and steroid 11β-hydroxylase (CYP11B1) has enabled the identification aldosterone-producing cell clusters (APCCs) in subcapsular portion human adult adrenal cortex. We hypothesized that adrenals have layered zonation early postnatal stages are remodeled to possess APCCs over time. Purposes. To investigate changes adrenocortical with age. Methods. retrospectively analyzed tissues prepared from 33 autopsied patients...
Significance A variety of rare mutations account for 10–20% EGFR in nonsmall cell lung cancer. However, due to high diversity, proper medication patients with such is impossible daily clinic. To appropriately treat cancer harboring mutations, a robust prediction model predict sensitivities mutants existing drugs strongly needed. Using molecular dynamics simulation-based model, we successfully predicted diverse exon 20 insertion inhibitors. The findings suggest the usefulness silico...
Abstract EGFR-mutated lung cancer accounts for a significant proportion of cases worldwide. For these cases, osimertinib, third-generation EGFR tyrosine kinase inhibitor, is extensively used as first-line or second-line treatment. However, cells acquire resistance to osimertinib in 1 2 years. Thus, thorough clarification mechanisms highly anticipated. Recent next-generation sequencing (NGS) samples identified several genetically defined such C797S MET amplification. nongenetically are not...
A circadian pacemaker generates a rhythm with period of approximately 24 hr even in the absence environmental time cues. Several photosensitive neuronal tissues such as retina and pineal gland contain autonomous together photic-input pathway responsible for entrainment to daily light/dark cycle. We show here that, constant darkness, chick mitogen-activated protein kinase (MAPK) exhibited an vivo tyrosine phosphorylation enzymatic activity peak during subjective night. Phosphorylated hence...
In a substantial population of non-small cell lung cancer (NSCLC), expression and activation EGF receptor (EGFR) have been reported is regarded as novel molecular target. A growing body evidence has shown the signaling crosstalk between EGFR integrins in cellular migration invasion. NEDD9 an integrin adaptor protein composed multiple domains serving substrate for variety tyrosine kinases. present study, we aimed at elucidating role integrins.Using NSCLC lines, conducted immunoblotting...
We recently isolated vasohibin-1 (VASH1), a novel angiogenic molecule that is specifically expressed in activated vascular endothelial cells (ECs), and the status of VASH1 expression has been documented various cancer angiogenesis. The aim this study was to assess prognostic value prostate (PCa). In study, we retrospectively analysed clinical records evaluated tumour microvessels 167 patients with PCa who underwent radical prostatectomy. immunohistochemically examined positive for anti-CD34...
Abstract Transdifferentiation of lung adenocarcinoma to small cell cancer (SCLC) has been reported in a subset cases that bear EGFR mutations. Several studies have the prerequisite role TP53 and RB1 alterations transdifferentiation. However, mechanism underlying transdifferentiation remains understudied, definitive additional events, third hit, for not yet identified. In addition, no prospective experiments provide direct evidence this study, we show FGF9 upregulation plays an essential An...
Contractility of smooth muscle and non-muscle microfilaments involves phosphorylation myosin II light chain. Myosin chain phosphatase (MLCP) is specifically inhibited by the protein kinase C-potentiated inhibitor 17 kDa, called CPI-17, as part Ca<sup>2+</sup> sensitization vascular contraction. Phosphorylation Thr<sup>38</sup> in CPI-17 enhances inhibitory potency toward MLCP over 1000-fold. In this study we mapped regions required for inhibition investigated mechanism using deletion point...
Endoscopic ultrasonography (EUS) was performed in 40 patients with pancreatic masses who subsequently underwent surgical resection, and we compared the ultrasonograms histopathologic findings. Ductal adenocarcinoma: The cephalad margin between tumor tissue distinct, while caudal blurred because severe fibrosis accompanying secondary pancreatitis, contour of irregular. internal echo pattern hypoechoic, an uneven central echogenic portion corresponding to irregularly arranged carcinomatous...
Reported herein is the case of a 74-year-old man with an unusual gastric carcinoma that developed at lesser curvature stomach. The tumor consisted small clusters cells surrounded by clear spaces, histopathology similar to invasive micropapillary (IMPC) breast. cells, which had downregulation E-cadherin expression, invaded subserous tissue and metastasized perigastric lymph nodes. IMPC, subtype breast carcinoma, known have frequent node metastases, resulting in poor clinical outcome. Although...
Abstract Thiazoline-related innate fear-eliciting compounds (tFOs) orchestrate hypothermia, hypometabolism, and anti-hypoxia, which enable survival in lethal hypoxic conditions. Here, we show that most of these effects are severely attenuated transient receptor potential ankyrin 1 ( Trpa1 ) knockout mice. TFO-induced hypothermia involves the -mediated trigeminal/vagal pathways non- olfactory pathway. TFOs activate -positive sensory projecting from trigeminal vagal ganglia to spinal nucleus...
Extracellular signal-regulated kinase (ERK) and p38 are members of the mitogen-activated protein (MAPK) family, in some cases these kinases serve for closely related cellular functions within a cell. In wide range animal clock structures, ERK plays an important role circadian time-keeping mechanism. Here we found that immunoreactivity to was uniformly distributed among cells chick pineal gland. On other hand, constant level activated detected over day, predominantly follicular parafollicular...
We have measured the densities of 1s5 and 1s3 argon metastables as a function abundance molecular oxygen in an inductively coupled plasma (ICP) mixtures Ar O2. Laser absorption spectroscopy was used to determine metastables. It found that even small abundances lead large increases metastable density, mostly due reduction electron number since electron-induced quenching determines density. At higher than 7% 15% for powers between 50 150 W, by molecules begins dominate density drops again.
DNA replication is a key step in initiating cell proliferation. Loading hexameric complexes of minichromosome maintenance (MCM) helicase onto origins during the G1 phase essential for replication. Here, we examined MCM hexamer states cycle human hTERT-RPE1 cells using multicolor immunofluorescence-based, single-cell plot analysis, and biochemical size fractionation. Experiments involving cell-cycle arrest at release from revealed that double was formed via single progression. A recruited to...
In this study we aimed to develop an artificial intelligence-based model for predicting postendoscopic retrograde cholangiopancreatography (ERCP) pancreatitis (PEP).
Chick pineal mitogen‐activated protein kinase (MAPK) exhibits circadian activation and light‐dependent deactivation at nighttime. Here we report that, in the chick gland, levels of active forms MAPK, MEK, Raf‐1 Ras exhibited synchronous rhythms with peaks during subjective night, suggesting a sequential components classical Ras‐MAPK pathway manner. In contrast, MAPK was not accompanied by any change MEK activity, but it attributed to phosphatase dephosphorylating MAPK. These results indicate...