A5045538077- Peptidase Inhibition and Analysis
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Protease and Inhibitor Mechanisms
- Ubiquitin and proteasome pathways
- Neuropeptides and Animal Physiology
- Occupational and environmental lung diseases
- Systemic Lupus Erythematosus Research
- CAR-T cell therapy research
- Chemokine receptors and signaling
- Signaling Pathways in Disease
- Neuroendocrine Tumor Research Advances
- Galectins and Cancer Biology
- Cancer-related gene regulation
- Cancer Immunotherapy and Biomarkers
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- T-cell and Retrovirus Studies
- Estrogen and related hormone effects
- Lung Cancer Research Studies
- NF-κB Signaling Pathways
Juntendo University
2015-2024
Juntendo University Hospital
2015-2024
Konan University
2024
Saitama Medical University
2024
Tokyo Metropolitan Bokutoh Hospital
2021
Respiratory Clinical Trials
2021
Nihon University
2021
University of Florida
2021
The University of Tokyo
2008-2018
Tokyo Medical University
2003-2016
Immunization of mice with lower primate lymphoid cells has provided a useful strategy for raising monoclonal antibodies against functionally important surface determinants on human T lymphocytes. We have developed antibody, anti-2H4, which defines unique cell subsets. This anti-2H4 antibody was reactive approximately 42% unfractionated cells, 41% T4+ inducer and 54% T8+ cytotoxic/suppressor population. Anti-2H4 not thymocytes, but reacted subsets peripheral blood B null cells. subdivided...
Monoclonal antibody anti-4B4 was produced by fusing NS1 myeloma with spleen cells of a mouse immunized Saguinus oedipus lymphocyte. This defines 135-KD cell surface protein that is widely distributed throughout the hematopoietic system. More importantly, reactive functionally unique human T subsets. Anti-4B4 approximately 41% unfractionated cells, T4+ inducer and 43% T8+ cytotoxic/suppressor population. subdivided peripheral blood into two distinct populations. The T4+4B4+ subset...
CD26, the T cell activation molecule dipeptidyl peptidase IV (DPPIV), associates with a 43-kilodalton protein. Amino acid sequence analysis and immunoprecipitation studies demonstrated that this protein was adenosine deaminase (ADA). ADA coexpressed CD26 on Jurkat lines, an in vitro binding assay showed through extracellular domain of CD26. deficiency causes severe combined immunodeficiency disease (SCID) humans. Thus, (DPPIV) interact surface, interaction may provide clue to pathophysiology...
Occupancy of the T-cell receptor complex does not appear to be a sufficient stimulus induce T-cell-mediated immune response. Increasing evidence suggests that cognate cell-cell interaction between an activated T cell and antigen-presenting may provide such stimulus. A candidate surface molecule for this costimulatory signal is T-cell-restricted CD28 antigen. Following crosslinking with anti-CD28 mAb, suboptimally stimulated CD28+ cells show increased proliferation markedly secretion subset...
Diet composition alters the metabolic states of adipocytes and hepatocytes in diabetes. The effects dipeptidyl peptidase-4 (DPP-4) inhibition on adipose tissue inflammation fatty liver have been obscure. We investigated extrapancreatic DPP-4 visceral fat liver.We diet-induced changes β-cell-specific glucokinase haploinsufficient (Gck(+/-)) diabetic mice. challenged animals with a diet containing combination sucrose oleic acid (SO) or linoleic (SL). Next, we assessed inhibitor,...
Senescent cell accumulation in aging tissues is linked to age-associated diseases and declining function, prompting efforts eliminate them. Mass spectrometry analysis revealed that DPP4 (dipeptidyl peptidase 4) was selectively expressed on the surface of senescent, but not proliferating, human diploid fibroblasts. Importantly, differential presence allowed flow cytometry-mediated isolation senescent cells using anti-DPP4 antibodies. Moreover, antibody-dependent cell-mediated cytotoxicity...
p130Cas (Cas) has been recently identified as a 130-kDa protein that is highly phosphorylated on tyrosine residues and stably associated with p47v-crk (v-Crk) p60v-src(v-Src) oncogene products in cells transformed by the respective genes. Cas novel signaling molecule having single Src homology (SH) 3 domain cluster of multiple SH2-binding motifs. While tight association v-Crk v-Src strongly suggestive significant role regulating cellular transformation, function normal untransformed totally...
B cell immunoglobulin production is regulated by helper T cells through direct interaction and secreted cytokines. In the present study, we functionally analyzed CD27 in cord peripheral blood cells. Adult were separated into CD27+ CD27- cells, which differed their morphology. Cord did not express CD27, expression on increased with age. Only had ability to produce immunoglobulin, was contact a tumor necrosis factor-related transmembrane ligand, CD70. can be further subdivided two discrete...
A monoclonal antibody termed anti-T4 that detected approximately 60% of peripheral blood T lymphocytes was shown to define the human inducer population. In present study, we characterized three additional antibodies, anti-T4A, anti-T4B, and anti-TQ1, were reactive with a similar percentage lymphocytes. Anti-T4A, delineated identical cell populations, while those defined by anti-TQ1 differed in several respects: 1) Anti-TQ1 stained minority (less than 7%) thymocytes, whereas other antibodies...
Focal adhesion kinase (pp125FAK) is localized to focal adhesions and tyrosine phosphorylated by the engagement of beta 1 integrins. However, it unclear how pp125FAK linked integrin molecules. We demonstrate that directly associated with paxillin, a 68-kD cytoskeleton protein. The COOH-terminal domain spanning FAK residues 919-1042 sufficient for paxillin binding has vinculin-homologous amino acids, which are essential binding. Microinjection subsequent immunohistochemical analysis reveal...
CD8+ (suppressor/cytotoxic) lymphocytes block replication of HIV-1 or the simian immunodeficiency virus macaques (SIVmac) in PBL infected individuals. We now show that these undergo clonal expansion vivo after AIDS infection individual, suggesting they may be antigen-specific T cells. These cells autologous but not MHC class I-mismatched PBL. The inhibitory express integrin family molecule 4B4 and CTL-associated S6F1 epitope LFA-1. Finally, physical contact is required for...
The T4+ lymphocyte population includes a subset that induces suppressor T lymphocytes (T8+ cells) and can be distinguished by dual-color fluorescence analysis with anti-2H4 anti-T4 monoclonal antibodies. To investigate the possible role of these cells in multiple sclerosis, we used antibody to characterize peripheral-blood subsets 63 patients sclerosis was progressive, stable, or acute (relapsing-remitting). Twenty-three 37 progressive had selective decrease number percentage induce (T4+2H4+...
CD27 is a member of the tumor necrosis factor (TNF) receptor superfamily and expressed on T, B, NK cells. The signal via plays pivotal roles in T-T T-B cell interactions. Here we demonstrate that overexpression activates NF-kappaB stress-activated protein kinase (SAPK)/c-Jun N-terminal (JNK). Deletion analysis cytoplasmic domain revealed C-terminal PIQEDYR motif was indispensable for both SAPK/JNK activation also required interaction with TNF receptor-associated (TRAF) 2 TRAF5, which have...
We previously showed that fibronectin (FN) synergized with anti-CD3 in induction of CD4+ T cell proliferation, and VLA-5 acted as a functional FN receptor serum-free culture system. In the present study, we VLA-4 is also involved this CD3-dependent CD4 activation through its interaction alternatively spliced CS1 domain FN. When highly purified cells were cultured on plates coated plus synthetic peptide-IgG conjugate, significant proliferation could be observed. Neither alone nor induced...
Abstract CD26, a T cell activation Ag, also known as dipeptidyl peptidase IV, is directly associated with adenosine deaminase (ADA) on the surface of cells and lines. In present study, we examined both binding ADA CD26 functional consequences this interaction. We found that was lines lacking either or IV enzymatic activity, indicating association between did not require activity. Moreover, using immunoelectron microscopy, demonstrated co-localized surface, but inside cells, suggesting...
In the present report, we demonstrated that modulation of CD26 from T cell surface induced by antiCD26 (1F7) led to enhanced phosphorylation CD3 zeta tyrosine residues and increased CD4 associated p56lck kinase activity. We further showed was comodulated on with CD45, a known membrane-linked protein phosphatase anti-CD26 capable precipitating CD45 lysates. These findings strongly suggest may be closely support notion interaction results in activity, chain phosphorylation, activation.
The cell surface phenotype of peripheral blood lymphocytes (PBL) systemic lupus erythematosus (SLE) patients was characterized with the anti-2H4 monoclonal antibody that defines human suppressor inducer subset. T4+2H4+ population cells has been shown to be critical for activation T8+ cells. Patients SLE a markedly decreased percentage (13 +/- 2%) in their PBL compared normal controls (21 1%) (P less than 0.001). This reduction greatest active SLE, especially those renal disease. Serial...
The CD45 Ag family is comprised of at least five isoforms generated by the alternative splicing three exons encoded a single leukocyte common gene. These isoforms, differentially expressed on subsets T cells, are widely believed to delineate maturational status. Thus, cells expressing high molecular mass CD45RA+ (220 and 205 kDa) represent naive whereas those lacking CD45RA density CD45RO (180 memory cells. This based findings that after activation, "convert" CD45RA- phenotype, losing...