A5044760409- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sexual Differentiation and Disorders
- Growth Hormone and Insulin-like Growth Factors
- Genetic factors in colorectal cancer
- Neuropeptides and Animal Physiology
- Photoreceptor and optogenetics research
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Semiconductor Quantum Structures and Devices
- Biofield Effects and Biophysics
- Pancreatic function and diabetes
- Colorectal Cancer Treatments and Studies
- Hormonal and reproductive studies
- Colorectal Cancer Surgical Treatments
- Hypothalamic control of reproductive hormones
- Fullerene Chemistry and Applications
- Peptidase Inhibition and Analysis
- Gender Studies in Language
- Epigenetics and DNA Methylation
- Connective tissue disorders research
- Cancer, Hypoxia, and Metabolism
- Adipose Tissue and Metabolism
- Diabetes and associated disorders
- Glycosylation and Glycoproteins Research
- Fibroblast Growth Factor Research
Sapporo Medical University
2022-2025
University of Hyogo
2024-2025
Tokyo Institute of Technology
2009-2022
Foundation for Growth Science
1993-2021
The University of Tokyo
2012-2021
Kyorin University
2003-2021
Tanaka Holdings (Japan)
2009-2021
Hitachi (Japan)
1994-2019
Ibaraki University
2019
Graz University of Technology
2019
Japanese mortality due to colorectal cancer is on the rise, surpassing 49,000 in 2015. Many new treatment methods have been developed during recent decades. The Society for Cancer of Colon and Rectum Guidelines 2016 (JSCCR 2016) were prepared show standard strategies cancer, eliminate disparities among institutions terms treatment, unnecessary insufficient deepen mutual understanding between health-care professionals patients by making these available general public. These consensus reached...
Abstract The number of deaths from colorectal cancer in Japan continues to increase. Colorectal exceeded 50,000 2016. In the 2019 edition, revision all aspects treatments was performed, with corrections and additions made based on knowledge acquired since 2016 version (drug therapy) 2014 (other treatments). Japanese Society for Cancer Colon Rectum guidelines treatment (JSCCR 2019) have been prepared show standard strategies cancer, eliminate disparities among institutions terms treatment,...
To explore the origins and consequences of tetraploidy in African clawed frog, we sequenced Xenopus laevis genome compared it to related diploid X. tropicalis genome. We characterize allotetraploid origin by partitioning its into two homoeologous subgenomes, marked distinct families 'fossil' transposable elements. On basis activity these elements age hundreds unitary pseudogenes, estimate that progenitor species diverged around 34 million years ago (Ma) combined form an 17–18 Ma. More than...
CD26, the T cell activation molecule dipeptidyl peptidase IV (DPPIV), associates with a 43-kilodalton protein. Amino acid sequence analysis and immunoprecipitation studies demonstrated that this protein was adenosine deaminase (ADA). ADA coexpressed CD26 on Jurkat lines, an in vitro binding assay showed through extracellular domain of CD26. deficiency causes severe combined immunodeficiency disease (SCID) humans. Thus, (DPPIV) interact surface, interaction may provide clue to pathophysiology...
Pseudovitamin D–deficiency rickets is characterized by the early onset of with hypocalcemia and thought to be caused a deficit in renal 25-hydroxyvitamin D3 1α-hydroxylase, key enzyme for synthesis 1α,25-dihydroxyvitamin D3.
The fifth component of the COP9 signalosome complex, Jab1/CSN5, directly binds to and induces specific down-regulation cyclin-dependent kinase inhibitor p27 (p27Kip1). Nuclear-cytoplasmic translocation plays an important role because leptomycin B (LMB), a chemical CRM1-dependent nuclear export, prevents degradation mediated by Jab1/CSN5. Here we show that Jab1/CSN5 functions as adaptor between CRM1 induce export subsequent degradation. but not p27, contains typical leucine-rich signal (NES)...
We report on the clinical and molecular findings in 25 males three females with Kallmann syndrome (KS) aged 10–53 yr. Ten were from five families, remaining 15 apparently sporadic cases. Molecular studies performed for 1 (KAL1) fibroblast growth factor receptor (FGFR1, also known as KAL2) by sequence analysis all coding exons, PCR-based deletion analysis, fluorescence situ hybridization (FISH) showing six novel two recurrent intragenic KAL1 mutations seven familial four male cases FGFR1 In...
Abstract CD26, a T cell activation Ag, also known as dipeptidyl peptidase IV, is directly associated with adenosine deaminase (ADA) on the surface of cells and lines. In present study, we examined both binding ADA CD26 functional consequences this interaction. We found that was lines lacking either or IV enzymatic activity, indicating association between did not require activity. Moreover, using immunoelectron microscopy, demonstrated co-localized surface, but inside cells, suggesting...
A novel murine cDNA which encodes a protein designated neuropsin was cloned. Northern and in situ hybridization analyses demonstrated that mRNA is expressed specifically the limbic system of mouse brain localized at highest concentration pyramidal neurons hippocampal CA1-3 subfields. Direct stimulation kindling induced by amygdaloid caused significant bilateral change level neurons. The activity-dependent changes specific localization indicate involved plasticity.
In the present report, we demonstrated that modulation of CD26 from T cell surface induced by antiCD26 (1F7) led to enhanced phosphorylation CD3 zeta tyrosine residues and increased CD4 associated p56lck kinase activity. We further showed was comodulated on with CD45, a known membrane-linked protein phosphatase anti-CD26 capable precipitating CD45 lysates. These findings strongly suggest may be closely support notion interaction results in activity, chain phosphorylation, activation.
T cells have been shown to express CD26, a known ectoenzyme with dipeptidyl peptidase IV (DPPIV; EC 3.4.14.5) activity in its extracellular domain. CD26 can also deliver second costimulatory signal and contribute T-cell activation. In an earlier study, we established CD26-transfected Jurkat lines demonstrated that monoclonal antibody-mediated crosslinking of CD3 induced interleukin 2 (IL-2) production. To determine the contribution DPPIV enzymatic human cDNA was mutated so active-site serine...
Abstract A cDNA encoding the T cell activation Ag CD26 was isolated from human PHA-activated cells by using an expression cloning method. The nucleotide sequence obtained predicts a protein of 766 amino acids type II membrane topology, with six in cytoplasmic region. predicted acid 85% homologous to that dipeptidyl peptidase IV enzyme rat liver. Derivatives leukemic line Jurkat transfected plasmid were established. Characterization expressed revealed could be immunoprecipitated as 110-kDa...
PRL and other lactogenic hormones are potent mitogens in a lymphoma cell line derived from lymph node of an estrogenized Noble (Nb) rat. The present study demonstrates that these cells (designated Nb2 node) possess receptors bind only hormones. There approximately 12,000 receptor sites per cell. kinetics binding [125I]iodo-PRL exhibited by is unusual follows biphasic pattern. Binding reaches maximum 1 h at 37 C, followed rapid decline. This pattern was not observed if carried out the...
DEER (double electron−electron resonance) enables the observation of long-range dipole interactions (1.5−8 nm) between electron spin centers and has become a unique method for structural analysis site-directed spin-labeled (SDSL) proteins. The was applied to proteins inside eukaryotic cells, Xenopus laevis oocytes. measurements oocytes, into which SDSL-ubiquitin derivates were injected, gave rise interpretable signals allowed us perform in situ analyses interspin distances
Abstract We report on molecular and clinical findings in 10 Japanese patients (four males six females) from eight families (two pairs of siblings isolated cases) with Antley-Bixler syndrome accompanied by abnormal genitalia and/or impaired steroidogenesis. Direct sequencing was performed for all the 15 exons cytochrome P450 oxidoreductase gene (POR), showing two missense mutations (R457H Y578C), a 24-bp deletion mutation resulting loss nine amino acids creation one acid (L612_W620delinsR),...
To study insulin exocytosis by monitoring the single secretory granule motion, evanescent wave microscopy was used to quantitatively analyze final stage of with biphasic release. Green fluorescent protein-tagged transfected in MIN6 beta cells packed granules, which appeared preferentially dock plasma membrane. Upon fusion evoked secretagogues, revealed that fluorescence green brightened, spread (within 300 ms), and then vanished. Under KCl stimulation, represents 1st phase release,...
Studies on human LH receptors are difficult due to the limited availability of clinical samples. Recent cloning rat and porcine receptor cDNAs indicated that these binding sites single polypeptides G-protein-coupled family with seven transmembrane domains. Based conserved sequences receptors, we performed reverse transcription polymerase chain reaction, using ovarian mRNA as template obtained partial cDNA clones. Further screening a ovary library subsequent ligation individual clones...
A cDNA clone for an auxin-regulated gene was isolated from a tobacco mesophyll protoplast library by differential screening. Nucleotide, sequence analysis showed that the deduced product of gene, which we have designated par, is hydrophilic and composed 220 amino acids. No significant homology to other known proteins detected. The mRNA par approximately 900 bases long its accumulation detected in cultured protoplasts as early 30 min after addition 2,4-dichlorophenoxyacetic acid culture...
To determine whether the increase in glucose uptake following AMP-activated protein kinase (AMPK) activation adipocytes is mediated by accelerated GLUT4 translocation into plasma membrane, we constructed a chimera between and enhanced green fluorescent (GLUT4-eGFP) transferred its cDNA nucleus of 3T3-L1 adipocytes. Then, dynamics GLUT4-eGFP were visualized living cells means laser scanning confocal microscopy. It was revealed that stimulation with...
The addition of a soluble recombinant CD26 (sCD26) enhanced proliferation peripheral blood lymphocytes induced by the recall antigen tetanus toxoid. sCD26 itself did not provide mitogenic signal and augment proliferative response T cells to other stimuli such as phytohemagglutinin anti-CD3. Dipeptidyl peptidase IV-negative have this enhancement effect, implying requirement for enzyme activity. It was found that there exists large variation in levels human plasma sCD26/dipeptidyl IV vivo...