A5023436601- Developmental Biology and Gene Regulation
- Meat and Animal Product Quality
- TGF-β signaling in diseases
- Muscle Physiology and Disorders
- Chromosomal and Genetic Variations
- Microbial Inactivation Methods
- Bee Products Chemical Analysis
- Bone Tissue Engineering Materials
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Congenital heart defects research
- Fish Biology and Ecology Studies
- Genetic diversity and population structure
- Calpain Protease Function and Regulation
- Ubiquitin and proteasome pathways
- Exercise and Physiological Responses
- Plant Reproductive Biology
- Muscle metabolism and nutrition
- Biochemical effects in animals
- Bone Metabolism and Diseases
- Wnt/β-catenin signaling in development and cancer
- Cardiomyopathy and Myosin Studies
- Biotin and Related Studies
- Proteins in Food Systems
- Hippo pathway signaling and YAP/TAZ
Hiroshima University
2010-2024
Nagoya City University
2024
Astellas Pharma (Japan)
2018
Niigata University
1996-2014
Teikyo Heisei University
2009
Hokkaido University
1994-2004
Rockefeller University
1997-2000
University of Niigata Prefecture
1981-1998
Yokohama National University
1996
National Center of Neurology and Psychiatry
1992-1996
To explore the origins and consequences of tetraploidy in African clawed frog, we sequenced Xenopus laevis genome compared it to related diploid X. tropicalis genome. We characterize allotetraploid origin by partitioning its into two homoeologous subgenomes, marked distinct families 'fossil' transposable elements. On basis activity these elements age hundreds unitary pseudogenes, estimate that progenitor species diverged around 34 million years ago (Ma) combined form an 17–18 Ma. More than...
Bone morphogenetic proteins (BMPs) are secreted that interact with cell-surface receptors and believed to play a variety of important roles during vertebrate embryogenesis. Bmpr, also known as ALK-3 Brk-1, encodes type I transforming growth factor-beta (TGF-beta) family receptor for BMP-2 BMP-4. Bmpr is expressed ubiquitously early mouse embryogenesis in most adult tissues. To study the function mammalian development, we generated Bmpr-mutant mice. After embryonic day 9.5 (E9.5), no...
Bone morphogenetic proteins (BMPs), which are members of the transforming growth factor beta (TGF-beta) superfamily, have been implicated in bone formation and regulation early development. To better understand roles BMPs Xenopus laevis embryogenesis, we cloned a cDNA coding for serine/threonine kinase receptor that binds BMP-2 BMP-4. analyze its function, attempted to block BMP signaling pathway embryos by using dominant-negative mutant receptor. When lacking putative domain was expressed...
Removal of rabbit psoas strips immediately after death and incubation in a saline solution containing 1 mM Ca2+ 5 nM Mg2+ for 9 hr at 37°C pH 7.1 causes complete Z-line removal but has no ultrastructurally detectable effect on other parts the myofibril. Z lines remain intact if 1,2-bis-(2-dicarboxymethylaminoethoxy)-ethane (EGTA) is substituted conditions unchanged. are broadened amorphous still present ethylenediaminetetraacetate (EDTA) solution. A protein fraction that from myofibrils...
ABSTRACT Morphogens are thought to establish pattern in early embryos by specifying several cell fates along a gradient of concentration; well-studied example is the Drosophila protein decapentaplegic (DPP) acting wing disc. Recent work has established that bone morphogenetic 4 (BMP4), vertebrate homologue DPP, controls fundamental choice between neural and epidermal ectoderm, under control antagonists secreted organizer region mesoderm. We now show BMP4 can act as morphogen, evoking...
ABSTRACT Epidermal fate in Xenopus ectoderm has been shown to be induced by a secreted growth factor, Bone Morphogenetic Protein 4 (BMP4). However, the molecular mechanism mediating this response is poorly understood. Here, we show that expression of homeobox gene, msx1, an immediate early BMP4 embryos. The timing and embryonic distribution msx1 parallel those described for BMP4. Moreover, over-expression embryos leads their ventralization as Consistent with BMP type signaling,...
Direct interaction between the C-terminal portion of dystrophin and dystrophin-associated proteins was investigated. The binding to each protein reconstituted by overlaying bacterially expressed fusion onto blot membranes which were transferred after separation SDS/PAGE with following results. (a) Among components glycoprotein complex links sarcolemma, a 43-kDa binds directly dystrophin. Although at least one sites this resides within cysteine-rich domain dystrophin, contribution additional...
ABSTRACT During limb development, the mesenchymal cells in restricted areas of bud, anterior necrotic zone, posterior opaque zone and interdigital zones, are eliminated by programmed cell death. The transcripts bone morphogenetic protein (Bmp)-2 -4 were first detected where death was observed, then showed overlapping expression with zones except zone. To investigate function BMP-2 BMP-4 during pattern formation, dominant negative form BMP receptor overexpressed chick leg bud via a...
Dystrophin is purified as a complex with several proteins from the digitonin‐solubilized muscle cell membrane. Most of dystrophin‐associated (DAPs) are assumed to form large oligomeric transmembranous glycoprotein on sarcolemma and link dystrophin basement membrane protein, laminin. In present study, we found that dystrophin‐DAP was dissociated into groups by n ‐octyl‐β‐ d ‐glucoside treatment. particular, stated above two distinct groups: one composed 156DAG 43DAG (A3a) other 50DAG, 35DAG...
Dystrophin, a protein product of the Duchenne muscular dystrophy gene, is thought to associate with muscle membrane by way glycoprotein complex which was co‐purified dystrophin. Here, we firstly demonstrate direct biochemical evidence for association carboxy‐terminal region dystrophin complex. The binding site found lie further inward than previously expected and confined cysteine‐rich domain first half domain. Since this portion corresponds well that, when missing results in severe...
The carboxy-terminal region of dystrophin has been suggested to be crucially important for its function prevent muscle degeneration. We have previously shown that this is the locus interacts with sarcolemmal glycoprotein complex, which mediates membrane anchoring dystrophin, as well cytoplasmic peripheral protein, A0 and beta 1-syntrophin (Suzuki, A., M. Yoshida, K. Hayashi, Y. Mizuno, Hagiwara, E. Ozawa. 1994. Eur. J. Biochem. 220:283-292). In work, by using overlay assay technique...
The biological effects of endogenous bone morphogenetic protein 4 (BMP-4), a member the transforming growth factor beta family, on embryonic development Xenopus laevis were investigated by using functionally defective mutant BMP-4 receptor (delta mTFR11), which blocks BMP signaling pathway. Injection delta mTFR11 RNA into either animal pole area or ventral marginal cells at two-cell stage induced dorsal phenotype in explant mesoderm with tissue from 10+ embryo, even though normal fate this...
ABSTRACT The ascidian tadpole larva is thought to be close a prototype of the ancestral chordate. vertebrate body plan established by series inductive cellular interactions, whereas ascidians show highly determinate mode development. Recent studies however, suggest some roles cell-cell interaction during embryogenesis. To elucidate signaling molecules responsible for interaction, we isolated HrBMPb, an homologue bone morphogenetic protein (BMP) gene, from Halocynthia roretzi. amino acid...
Abstract This paper reports the experimental results of processes used for formation whipped oils composed vegetable (salad oil) and high‐melting fat crystals [fully hydrogenated rapeseed oil rich in behenic acid (FHR‐B)]. No emulsifier was added to form this oil. Microprobe FT‐IR spectroscopy, synchrotron radiation microbeam X‐ray diffraction (SR‐μ‐XRD), polarized optical microscopy, differential scanning calorimetry (DSC) were employed observe fine crystal particles most stable polymorph β...