A5050015025- Sleep and Wakefulness Research
- Neuroscience of respiration and sleep
- Neuroscience and Neuropharmacology Research
- Circadian rhythm and melatonin
- Sleep and related disorders
- Genetics and Neurodevelopmental Disorders
- Memory and Neural Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Neuroendocrine regulation and behavior
- Adenosine and Purinergic Signaling
- Ion channel regulation and function
- Advanced biosensing and bioanalysis techniques
- Restless Legs Syndrome Research
- Neural dynamics and brain function
- Autism Spectrum Disorder Research
- Biotin and Related Studies
- Regulation of Appetite and Obesity
- Receptor Mechanisms and Signaling
- Glioma Diagnosis and Treatment
- Pain Mechanisms and Treatments
- Vagus Nerve Stimulation Research
- Infant Health and Development
- Neonatal and fetal brain pathology
- Cholesterol and Lipid Metabolism
Fudan University
2017-2024
Jinshan Hospital of Fudan University
2024
State Key Laboratory of Medical Neurobiology
2017-2023
Shanghai Medical College of Fudan University
2013-2023
Shanghai Center for Brain Science and Brain-Inspired Technology
2018
Allen Institute for Brain Science
2017
Nucleus accumbens (NAc) is involved in behaviors that depend on heightened wakefulness, but its impact arousal remains unclear. Here, we demonstrate NAc dopamine D
Dysfunction of the striatum is frequently associated with sleep disturbances. However, its role in sleep-wake regulation has been paid little attention even though densely expresses adenosine A2A receptors (A2ARs), which are essential for adenosine-induced sleep. Here we showed that chemogenetic activation A2AR neurons specific subregions induced a remarkable increase non-rapid eye movement (NREM) Anatomical mapping and immunoelectron microscopy revealed striatal innervated external globus...
The rostromedial tegmental nucleus (RMTg), also called the GABAergic tail of ventral area, projects to midbrain dopaminergic system, dorsal raphe nucleus, locus coeruleus, and other regions. Whether RMTg is involved in sleep–wake regulation unknown. In present study, pharmacogenetic activation rat neurons promoted non-rapid eye movement (NREM) sleep with increased slow-wave activity (SWA). Conversely, rats after neurotoxic lesions 8 or 16 days showed decreased NREM reduced SWA at lights on....
Adenosine A2A receptors (A2ARs) in the nucleus accumbens (Acb) have been demonstrated to play an important role arousal effect of adenosine receptor antagonist caffeine, and may be involved physiological sleep. To better understand functions these sleep, projections A2AR neurons were mapped utilizing adeno-associated virus (AAV) encoding humanized Renilla green fluorescent protein (hrGFP) as a tracer for long axonal pathways. The Cre-dependent AAV was injected into core (AcbC) shell (AcbSh)...
Abstract Hypoglossal motor neurons (HMNs) innervate tongue muscles and play key roles in a variety of physiological functions, including swallowing, mastication, suckling, vocalization, respiration. Dysfunction HMNs is associated with several diseases, such as obstructive sleep apnea (OSA) sudden infant death syndrome. OSA serious breathing disorder the activity during different sleep–wake states. Identifying neural mechanisms by which state-dependent activities are controlled may be helpful...
The suprachiasmatic nucleus (SCN) is the principal pacemaker driving circadian rhythms of physiological behaviors. SCN consists distinct neurons expressing neuropeptides, including arginine vasopressin (AVP), vasoactive intestinal polypeptide (VIP), gastrin-releasing peptide (GRP), cholecystokinin (CCK), and so on. AVP, VIP, GRP receive light stimulation from retina to synchronize endogenous clocks with solar day, whereas CCK are not directly innervated by retinal ganglion cells may be...
Abstract Objective Phenylethanolamine N ‐methyltransferase (PNMT)‐expressing neurons in the nucleus tractus solitarii (NTS) contribute to regulation of autonomic functions. However, neural circuits linking these other brain regions remain unclear. This study aims investigate connectivity mechanisms PNMT‐expressing NTS (NTS PNMT neurons). Methods The methodologies employed this included a modified rabies virus‐based retrograde tracing technique, conventional viral anterograde tracing, and...
Physiological rapid eye movement (REM) sleep termination is vital for initiating non-REM (NREM) or arousal, whereas the suppression of excessive REM promising in treating narcolepsy. However, neuronal mechanisms controlling and keeping continuation remain largely unknown. Here, we reveal a key brainstem region GABAergic neurons control both physiological cataplexy. Using fiber photometry optic tetrode recording, characterized dorsal part deep mesencephalic nucleus (dDpMe) as relatively...
Abstract Ethanol has extensive effects on sleep and daytime alertness, causing premature disability death. Adenosine, as a potent sleep-promoting substance, is involved in many cellular behavioral responses to ethanol. However, the mechanisms of hypnotic ethanol remain unclear. In this study, we investigated role adenosine ethanol-induced using C57BL/6Slac mice, A 2A receptor (A R) knockout their wild-type littermates. The results showed that intraperitoneal injection (3.0 g/kg) at 21:00...
The GABAergic neurons in the lateral pontine tegmentum (LPT) play key roles regulation of sleep and locomotion. dysfunction LPT is related to neurological disorders such as rapid eye movement behavior disorder ocular flutter. However, whole-brain neural connectivity remains poorly understood. Using virus-based, cell-type-specific, retrograde anterograde tracing systems, we mapped monosynaptic inputs axonal projections mice. We found that received mainly from superior colliculus, substantia...
Patients with monoallelic bromodomain and PHD finger-containing protein 1 ( BRPF1 ) mutations showed intellectual disability. The hippocampus has essential roles in learning memory. Our previous work indicated that Brpf1 was specifically strongly expressed the from perinatal period to adulthood. We hypothesized mouse plays critical morphology function of hippocampal neurons, its deficiency leads memory deficits. To test this, we performed immunofluorescence, whole-cell patch clamp, mRNA-Seq...
Abstract It is well known that the main forms of innervation are synapses and free nerve endings, while other have not been reported. Here, we explore a new way innervating lymphoid organs. Male Sprague‐Dawley rats were used for studying sympathetic fibers in lymph nodes by means anterograde tracking, immunoelectron microscopy, three‐dimension reconstruction analysis, immunofluorescence labeling. The results showed Fluoro‐Ruby labeled endings targeted only group cells entered through plasma...
Ubiquitination is a major posttranslational modification of proteins that affects their stability, and E3 ligases play key role in ubiquitination by specifically recognizing substrates. BTBD9, an adaptor the Cullin-RING ligase complex, responsible for substrate recognition associated with sleep homeostasis. However, substrates BTBD9-mediated remain unknown. Here, we generated SH-SY5Y cell line stably expressing BTBD9 performed proteomic analysis combined ubiquitinome to identify downstream...
Abstract The cholinergic neurons in the nucleus basalis of Meynert (NBM) are a key structure cognition, dysfunction which is associated with various neurological disorders, especially dementias. However, whole‐brain neural connectivity to NBM remains be further and comprehensively researched. Using virus‐based, specific, retrograde, anterograde tracing, we illustrated monosynaptic inputs axon projections choline acetyltransferase (ChAT)‐Cre transgenic mice. Our results showed that received...