A5042514704- Sleep and Wakefulness Research
- Circadian rhythm and melatonin
- Sleep and related disorders
- Neuroscience and Neuropharmacology Research
- Neuroscience of respiration and sleep
- Olfactory and Sensory Function Studies
- Anesthesia and Neurotoxicity Research
- Adenosine and Purinergic Signaling
- Mast cells and histamine
- Neurotransmitter Receptor Influence on Behavior
- Pain Mechanisms and Treatments
- Stress Responses and Cortisol
- Regulation of Appetite and Obesity
- Neuroendocrine regulation and behavior
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Neural dynamics and brain function
- Biochemical Analysis and Sensing Techniques
- Memory and Neural Mechanisms
- EEG and Brain-Computer Interfaces
- Anesthesia and Sedative Agents
- Neurogenesis and neuroplasticity mechanisms
- Neurological disorders and treatments
- Neuropeptides and Animal Physiology
- Parkinson's Disease Mechanisms and Treatments
Fudan University
2016-2025
Zhongshan Hospital
2023-2025
The First Affiliated Hospital, Sun Yat-sen University
2023-2025
Sun Yat-sen University
2023-2025
State Key Laboratory of Medical Neurobiology
2015-2024
Institute of Neurobiology
2008-2024
Shanghai Medical College of Fudan University
2013-2023
China Southern Power Grid (China)
2023
Shenzhen Polytechnic
2022
Eye & ENT Hospital of Fudan University
2021
Orexin neurons are exclusively localized in the lateral hypothalamic area and project their fibers to entire central nervous system, including histaminergic tuberomammillary nucleus (TMN). Dysfunction of orexin system results sleep disorder narcolepsy, but role physiological sleep–wake regulation mechanisms involved remain be elucidated. Here we provide several lines evidence that A induces wakefulness by means TMN histamine H 1 receptor (H1R). Perfusion (5 25 pmol/min) for hr into rats...
Caffeine, the most widely used psychoactive compound, is an adenosine receptor antagonist. It promotes wakefulness by blocking A<sub>2A</sub> receptors (A<sub>2A</sub>Rs) in brain, but specific neurons on which caffeine acts to produce arousal have not been identified. Using selective gene deletion strategies based Cre/loxP technology mice and focal RNA interference silence expression of A<sub>2A</sub>Rs rats local infection with adeno-associated virus carrying short-hairpin RNA, we report...
Nucleus accumbens (NAc) is involved in behaviors that depend on heightened wakefulness, but its impact arousal remains unclear. Here, we demonstrate NAc dopamine D
Abstract Sleep control is ascribed to a two-process model, widely accepted concept that posits homoeostatic drive and circadian process as the major sleep-regulating factors. Cognitive emotional factors also influence sleep–wake behaviour; however, precise circuit mechanisms underlying their effects on sleep are unknown. Previous studies suggest adenosine has role affecting behavioural arousal in nucleus accumbens (NAc), brain area critical for reinforcement reward. Here, we show...
Abstract Flickering light stimulation has emerged as a promising non-invasive neuromodulation strategy to alleviate neuropsychiatric disorders. However, the lack of neurochemical underpinning hampered its therapeutic development. Here, we demonstrate that flickering triggered an immediate and sustained increase (up 3 h after flickering) in extracellular adenosine levels primary visual cortex (V1) other brain regions, function frequency intensity, with maximal effects observed at 40 Hz 4000...
Abstract Transdermal drug delivery is of vital importance for medical treatments. However, user adherence to long-term repetitive poses a grand challenge. Furthermore, the dynamic and unpredictable disease progression demands pharmaceutical treatment that can be actively controlled in real-time ensure precision personalization. Here, we report spatiotemporal on-demand patch (SOP) integrates drug-loaded microneedles with biocompatible metallic membranes enable electrically triggered active...
Modafinil is a wake-promoting compound with low abuse potential used in the treatment of narcolepsy. Although reported to affect multiple neurotransmitter systems such as catecholamines, serotonin, glutamate, GABA, orexin, and histamine, however, molecular mechanism by which modafinil increases wakefulness debated. Herein we dopamine (DA) D 2 receptor (D R)-deficient mice combined 1 R- R-specific antagonists clarify role DA receptors arousal effects modafinil. In wild-type mice,...
Infusion of prostaglandin (PG) D 2 into the lateral ventricle brain induced an increase in amount non-rapid eye movement sleep wild-type (WT) mice but not deficient PGD receptor (DP). Immunofluorescence staining WT mouse revealed that DP immunoreactivity was dominantly localized leptomeninges (LM) basal forebrain synthase widely distributed LM entire brain. Electron microscopic observation indicated DP-immunoreactive particles were predominantly located on plasma membranes arachnoid...
Dopamine (DA) and its D 2 receptor (R) are involved in cognition, reward processing, drug addiction. However, their roles sleep–wake regulation remain unclear. Herein we investigated the role of R by using knock-out (KO) mice pharmacological manipulation. Compared with WT mice, KO exhibited a significant decrease wakefulness, concomitant increase non-rapid eye movement (non-REM, NREM) REM sleep drastic low-frequency (0.75–2 Hz) electroencephalogram delta power NREM sleep, especially during...
Histaminergic neurons play an important role in the regulation of sleep–wake behavior through histamine H 1 receptors (H R). Blockade 3 receptor R) is proposed to induce wakefulness by regulating release various wake-related transmitters, not only histamine. In present study, we characterized cycles R knockout (KO) mice and their arousal responses antagonist. Under baseline conditions, KO showed essentially identical those WT but with fewer incidents brief awakening (<16-sec epoch),...
Adenosine has been proposed to promote sleep through A 1 receptors (A R's) and/or 2A in the brain. We previously reported that mediate sleep-promoting effect of prostaglandin D 2 , an endogenous sleep-inducing substance, and activation these induces blockade them by caffeine results wakefulness. On other hand, R suggested increase inhibition cholinergic region basal forebrain. However, role target sites sleep–wake regulation remained controversial. In this study, immunohistochemistry...
Dysfunction of the striatum is frequently associated with sleep disturbances. However, its role in sleep-wake regulation has been paid little attention even though densely expresses adenosine A2A receptors (A2ARs), which are essential for adenosine-induced sleep. Here we showed that chemogenetic activation A2AR neurons specific subregions induced a remarkable increase non-rapid eye movement (NREM) Anatomical mapping and immunoelectron microscopy revealed striatal innervated external globus...
Sleep is regulated by two mechanisms: the homeostatic process and circadian clock. Light affects sleep alertness entraining clock, acutely inducing sleep/alertness, in a manner mediated intrinsically photosensitive retinal ganglion cells. Because cells are believed to be minimally sensitive red light, which widely used for illumination reduce photic disturbance nocturnal animals during dark phase. However, appropriate intensity of light unknown. In present study, we recorded...